Multi-cavity microsphere based on electronic injection method and preparation method of multi-cavity microsphere

Abstract

The invention discloses a multi-cavity microsphere based on an electronic injection method and a preparation method of the multi-cavity microsphere. The preparation method comprises the following steps: firstly, preparing a coaxial needle containing a core layer channel and a shell layer channel; secondly, preparing a sodium alginate solution and a mixed solution containing one or more loads; respectively sucking the mixed liquid by using the injector again, and pushing the mixed liquid into different nuclear layer channels of the coaxial needle; an additive-free sodium alginate solution is introduced into a shell layer channel; finally, the high-voltage power supply is connected with the metal part of the coaxial needle; the microsphere receiving device is connected with a copper plate of a grounding wire, a pump and a high-voltage power supply are turned on, the precursor solution which is formed at an outlet of the coaxial needle and is of a partitioned and layered structure falls into the receiving device, stirring and filtering are conducted, and a multi-cavity microsphere finished product is obtained. The multi-cavity microsphere with the core-shell structure is formed in one step, so that the loss of the loaded component in the preparation process is reduced, the protection and confinement of the loaded component are improved, meanwhile, the regulation and control of the release rate of the loaded drug can be realized, and the preparation method has the advantages in industrial production.

Description

technical field [0001] The invention relates to the technical field of biological material preparation, in particular to a multi-chamber microsphere based on an electrospray method and a preparation method thereof. Background technique [0002] Multi-chamber microspheres can load different drugs, cells or other factors in different spaces of a single microsphere, so they have many important application prospects in the fields of multi-drug release, cell co-culture, and multi-target detection. At present, microfluidic technology is the main method for preparing multi-chambered microspheres, but the microfluidic method requires the use of oils, initiators and surfactants, which have a certain impact on the activity of biological agents or cells to be loaded. The application of this method in the biological field is limited. [0003] In order to avoid the possible toxic effects of these oils, initiators, etc., new multi-chamber microsphere preparation methods have been explore...

AI Technical Summary

Problems solved by technology

The centrifugation method requires a special device, which is difficult to popularize, and the capacity of the centrifuge tube is limited, so the efficiency is also low

The gas shear law has the disadvantages of possibly introducing gas-derived pollution and high cost

Although the electrospray method is relatively simple and easy to obtain, the operation is simple and efficient, but the existing three methods cannot prepare multi-chamber microspheres with core-shell layering in one step.

At the same time, there is no difference in the structural composition of each chamber in the preparation of the existing multi-chamber microspheres, and it is impossible to control the release rate of drugs loaded in different chambers.

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