Fluorescent probe and application thereof to detection of five aromatic pesticides

A fluorescent probe, fluorescence technology, applied in the field of analytical chemistry

Pending Publication Date: 2022-06-24
GUIZHOU UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report on the multi-target detection of pesticides FBZ, CBZ, TBZ, TDZ, and TCZ using melon rings as the corresponding supramolecular complex fluorescent probes at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescent probe and application thereof to detection of five aromatic pesticides
  • Fluorescent probe and application thereof to detection of five aromatic pesticides
  • Fluorescent probe and application thereof to detection of five aromatic pesticides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Preparation of fluorescent probe solution

[0028] Reagents: ThT (analytical grade) was purchased from Shanghai Aladdin Reagent Company, and the melon ring was taken from the Key Laboratory of Supramolecular Macrocycle Chemistry of Guizhou University (purity ≥98%).

[0029] Method: Accurately weigh 13.3 mg Q[8] and 3.2 mg ThT mixed with ultrapure water to prepare 100 mL, the molar concentration ratio is 1:1, and the molar concentration is 1×10 -4 mol / L fluorescent probe solution.

[0030] Among them, Q[8] is a supramolecular complex with a molar concentration ratio of 1:1 formed by the weak supramolecular interaction force of ion-dipole and hydrophobic interaction with ThT, which is referred to as a fluorescent probe, thereby causing the fluorescent red of ThT. Move to 567nm and enhance, when there are pesticide molecules, the pesticide molecules form a new ternary charge transfer complex with the fluorescent probe, called ternary complex for short, which ca...

Embodiment 2

[0031] Example 2: Establishment of a multi-target detection method

[0032] Reagents: FBZ, CBZ, TBZ, TDZ, TCZ (analytical grade) were purchased from Shanghai Aladdin Reagent Company.

[0033] Method: The concentrations of fluorescent probes and pesticide molecules were kept constant at 2×10 -5 mol / L, fixed excitation wavelength of 408 nm, excitation slit of 5 nm, emission slit of 5 nm, voltage of 600 V, and measured the fluorescence emission spectrum of the system.

[0034] The aqueous solution of the fluorescent probe shows fluorescence at 567 nm. After adding aromatic pesticide molecules, FBZ and TBZ make the fluorescence emission peak of the system blue-shift from 567 nm to 498 nm, CBZ makes the fluorescence emission peak of the system blue-shift to 495 nm, and TCZ makes the fluorescence emission peak of the system blue-shifted. The emission peak was blue-shifted to 493 nm, and the intensity was enhanced by about 1-2 times. In addition, TDZ made the fluorescence blue-shift o...

Embodiment 3

[0038] Embodiment 3: Establishment of detection method

[0039] Method: The concentration of fluorescent probes was kept constant at 2 × 10 -5 mol / L, the concentration range of pesticide molecules is 2×10 -6 ~6×10 -5 mol / L, fixed excitation wavelength of 408 nm, slit 5 nm, and voltage of 600 V for fluorescence emission spectrum measurement, with the concentration of pesticide molecules as the abscissa, and the fluorescence emission spectrum intensity corresponding to 497 nm, 493 nm, 496 nm, 487 nm, and 493 nm as the ordinate to draw a standard curve .

[0040] According to the experimental results, the response linear ranges are: Maisuining and Thiabendazole: 0~5×10 -6 mol / L, carbendazim: 0~8×10 -6 mol / L, thidiazuron and tricyclazole: 0~7×10 -6 mol / L, the detection limits are: Mai Suining: 1.25×10 - 7 mol / L, carbendazim: 1.71×10 -7 mol / L, Thiabendazole: 1.17×10 -7 mol / L, Thidiazuron: 1.22×10 -7 mol / L, tricyclazole: 2.60×10 -7 mol / L, see attached for experimental res...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a fluorescent probe and application thereof to detection of five aromatic pesticides, according to the fact that a supramolecular compound generated by cucurbit [8] uril (Q [8]) and thioflavin T (ThT) can generate yellow fluorescence, and FBZ, CBZ, TBZ, TDZ and TCZ can make the yellow fluorescence blue shift and be enhanced, the fluorescent probe can be applied to detection of five aromatic pesticides. The supramolecular complex formed by Q [8] and ThT in a molar concentration ratio of 1: 1 is used as the fluorescent probe, so that five aromatic pesticides such as FBZ, CBZ, TBZ, TDZ and TCZ in the environment can be conveniently, quickly and sensitively detected.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, in particular to a fluorescent probe and its detection application to five kinds of aromatic pesticides; Supramolecular complexes, the application is to distinguish and identify fenugreek (FBZ), carbendazim (CBZ), thiabendazole (TBZ), thidiazuron (TDZ), and tricyclazole (TCZ) in water. Background technique [0002] Fluorescent molecular probes are favored because of their high sensitivity, low test cost, simple sample processing and operation, fast assay method, no need for expensive equipment, easy visualization and real-time detection. [0003] Aromatic pesticides, such as FBZ, CBZ, TBZ, TDZ, TCZ, etc., are fungicides widely used in agriculture. Because of their water solubility and non-volatile properties, they may remain in the environment and pose a serious threat to the ecological environment and human health. . [0004] The reported residual analysis methods of FBZ, CBZ, TBZ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 黄英韦凯妮唐青黄书珍陶朱
Owner GUIZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap