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Sample pretreatment method for detecting content of chloral hydrate in health care product and detection method of chloral hydrate

A sample pretreatment and chloral hydrate technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to shorten the required time, simplify the detection and analysis process, and eliminate matrix interference

Pending Publication Date: 2022-07-05
GUILIN MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Based on this, the purpose of the present invention is to provide a sample pretreatment method for the detection of chloral hydrate content in health products. The method of the present invention overcomes the difficult problem of separating the analyte from the complex system of health products, and the steps are simple and easy to operate. High sensitivity, rapid detection, suitable for rapid analysis of chloral hydrate in health products

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  • Sample pretreatment method for detecting content of chloral hydrate in health care product and detection method of chloral hydrate
  • Sample pretreatment method for detecting content of chloral hydrate in health care product and detection method of chloral hydrate
  • Sample pretreatment method for detecting content of chloral hydrate in health care product and detection method of chloral hydrate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Pretreatment of samples:

[0056] The present invention adopts the miniaturized heat-assisted sample pretreatment device disclosed in CN 105910881 B to pretreat the sample, and the device can realize the integration of acidolysis, heating and decomposition product reabsorption process of chloral hydrate. The device includes a carrier gas system, a heating chamber, a vaporization tank and a receiving pipe; the vaporization tank is arranged in the heating chamber for placing samples, the heating chamber is a cavity, and air inlets are provided at both ends of the heating chamber and an air outlet, and a sample inlet is provided above the cavity at a position corresponding to the gasification tank; the carrier gas system is connected to the heating chamber through the air inlet; the receiving tube is connected to the heating chamber through the air outlet, and the receiving The tube contains a solution that absorbs the vaporized sample.

[0057] Pre-processing the sample,...

Embodiment 2

[0061] The chloride ion and formic acid in the liquid to be tested were detected by ion chromatography, and the concentration of chloral hydrate in the test sample of the health product was calculated by the linear regression equation of formic acid and chloride ion.

[0062] Preparation of standard curve solution: prepare chloral hydrate standard solution with concentration gradient of 5.0, 10.0, 20.0, 30.0, 40.0, 50.0 μg / mL series. The chloral hydrate standard solution of a series of concentration gradients was respectively subjected to sample pretreatment according to the steps in Example 1 to obtain a corresponding standard curve solution.

[0063] The pretreatment of the test sample of the health care product: the test sample of the health care product is subjected to the sample pretreatment according to the steps in the embodiment 1, and its absorption liquid is diluted 40 times to obtain the corresponding test sample liquid to be tested.

[0064] Sample detection: Therm...

Embodiment 3

[0068] Hydrophilic interaction chromatography (HILIC, also known as reversed-phase chromatography) was used to detect formic acid, and the concentration of chloral hydrate in the test sample of health care products was calculated by the linear regression equation of formic acid.

[0069] Preparation of standard curve solution: prepare chloral hydrate standard solution with concentration gradient of 5.0, 10.0, 20.0, 40.0, 60.0, 80.0, 100.0 μg / mL series. The chloral hydrate standard solution of a series of concentration gradients is respectively subjected to sample pretreatment according to the steps in Example 1 to obtain a corresponding standard curve solution.

[0070] The pretreatment of the test sample of the health care product: the sample of the health care product is subjected to the sample pretreatment according to the steps in the embodiment 1, and its absorption liquid is diluted 40 times to obtain the corresponding test sample liquid to be tested.

[0071] Sample det...

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Abstract

The invention relates to a sample pretreatment method for detecting the content of chloral hydrate in a health-care product and a detection method of the chloral hydrate. The sample pretreatment method for detecting the content of chloral hydrate in the health care product comprises the following steps: taking a proper amount of sample, adding an acid solution, heating, and decomposing chloral hydrate to generate HCl and HCOOH gases; and absorbing the generated gas with water to obtain a solution to be detected. According to the method, chloral hydrate can be subjected to a hydrolysis reaction under acidic and heating conditions and generate hydrogen chloride and formic acid with high volatility, then the hydrogen chloride and the formic acid are separated by combining a gas-liquid separation technology, and the content of chloral hydrate in the health care product is determined by adopting an analysis technology for detecting chloride ions or formic acid. Compared with the prior art, the sample pretreatment method for detecting the content of chloral hydrate in the health care product and the detection method of the sample pretreatment method have the advantages that matrix interference can be effectively eliminated, the sample pretreatment time is shortened, the detection and analysis process of chloral hydrate in the health care product is greatly simplified, the steps are simple, and the sensitivity is high.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a sample pretreatment and detection method for detecting the content of chloral hydrate in a health product. Background technique [0002] Chloral hydrate, chemical name 2,2,2-trichloro-1,1-ethylene glycol, English name Chloral hydrate, CAS No. 302-17-0. [0003] Chloral hydrate has a hypnotic effect when used as a therapeutic drug, and the hypnotic effect is mild, does not shorten rapid eye movement sleep (REMS), and has no obvious sequelae; it is illegal to add it to health care products in order to improve efficacy. Health products have a regulating effect and are special foods that are not intended to treat diseases. The National Food Safety Law stipulates that drugs shall not be illegally added to health products. [0004] The detection technology of the chloral hydrate reported at present is mostly aimed at the crude drug, such as "method for the determination of ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N30/8675
Inventor 陈正毅袁锟婷田池
Owner GUILIN MEDICAL UNIVERSITY
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