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Tissue culture method of potentilla glandulifera

A technique of tissue culture and hair growth, which is applied in the field of plant tissue culture, can solve the problems of less raw materials required and large reproduction coefficient, and achieve the effects of good growth state, high culture efficiency, and convenient material collection

Active Publication Date: 2022-07-08
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no relevant research on the tissue culture technology of Potentilla trichomes at present. Compared with traditional propagation methods, tissue culture has the advantages of less raw materials required, short growth cycle, large reproduction coefficient, artificially controllable culture conditions, and isolation from natural conditions. It has outstanding advantages such as interference, and can cultivate a large number of high-quality seedlings with consistent traits in a short period of time, which is of great significance to the development of rural land cover plants

Method used

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  • Tissue culture method of potentilla glandulifera
  • Tissue culture method of potentilla glandulifera
  • Tissue culture method of potentilla glandulifera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Sterilization of explants

[0020] Taking the healthy plants of Cauliflower as the test material, cut young leaves, soak them in detergent water for 1 min, rinse them under running water for 30 min, put them on the ultra-clean workbench, disinfect them with 75% alcohol solution for 1 min, and then pour them out. After alcohol, rinse with sterile water for 3-4 times, then soak in 2% and 4% sodium hypochlorite solution for several minutes respectively (see Table 3.1 for the specific time), shake continuously during this period to make the explant and the solution fully contact, and then use Immerse in sterile water for 5-6 times, and dry the surface water with sterile filter paper. The leaves were cut into leaf discs of 0.5*0.5mm and inoculated on MS+6-BA0.5mg / L+2,4-D0.5mg / L medium. Each treatment was inoculated with 30 explants, repeated 3 times. After 3 weeks, the contamination rate, mortality rate and callus induction rate of different disinfection schemes w...

Embodiment 2

[0028] Example 2 Callus induction

[0029] The sterilized explants were inoculated into MS minimal medium supplemented with different concentrations of 6BA, 2,4D, NAA and TDZ, 30 explants per treatment in 3 replicates. After 30 days, the callus induction rate of the explants was counted, and the growth state and color of the callus were observed and recorded.

[0030] Table 3 Callus induction medium

[0031]

[0032]

[0033] It can be seen from Table 4 that the ratios of hormones in the medium are different, and the callus induction rate and callus state of the corresponding leaves of C. chinensis are significantly different. The results showed that when a single cytokinin 6-BA was added to the medium, no callus could be produced on the leaves regardless of the concentration of 6-BA.

[0034]On each medium with different 6-BA and 2.4D mass concentrations, the callus induction rate of the leaves of C. pilaris was significantly different (P<0.05). The mass concentratio...

Embodiment 3

[0044] Example 3 Callus differentiation

[0045] The callus with vigorous growth was selected and transferred to the differentiation medium, and the medium ratio is shown in Table 5. 10 calli were inoculated for each treatment with 3 replicates. After 30 days, the callus differentiation rate was counted, and the growth state and color of the callus were observed and recorded. If there were small buds, the growth state of the buds was recorded.

[0046] Table 5 Callus Differentiation Medium

[0047]

[0048]

[0049] The callus in good condition induced by the leaves of C. pilaris was inoculated on the differentiation medium, and the statistical results after 30 days are shown in Table 3.6. According to the data in the table, it can be seen that different hormone ratios have the same effect on the different hormones. Vegetable callus differentiation has an important impact, only 4 of the 18 different mediums successfully differentiated callus into adventitious buds, and...

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Abstract

The invention provides a tissue culture method of potentilla glandulosa, which is characterized by comprising the following steps: by taking tender leaves as explants, sterilizing the surfaces of the tender leaves, inoculating the tender leaves into a callus induction culture medium to induce calluses, and inoculating the induced calluses into an adventitious bud differentiation culture medium to differentiate adventitious buds, the callus induction culture medium is MS + NAA (Naphthalene Acetic Acid) 0.1-05mg / L and TDZ (Toluene Disulfide) 0.1-1.0 mg / L, and the adventitious bud differentiation culture medium is MS + NAA 0.5 mg / L and TDZ 0.1-1.0 mg / L. According to the method, the hormone combination of NAA and TDZ is applied in the callus induction stage of the tissue culture of the potentilla plant for the first time, the induction rate of the potentilla leaf callus can reach 90.00% or above through the induction method, the growth state of the callus is good, and obvious buds can be seen on the surface in the later culture stage. By using the callus differentiation medium disclosed by the invention, the differentiation rate can reach 46.67%, adventitious buds grow robustly, and a good foundation can be laid for successful tissue culture of the potentilla chinensis.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a tissue culture method of Cauliflower glandularis. Background technique [0002] Beijing is located in the North China Plain, with a typical north temperate continental monsoon climate, hot and rainy in summer and cold and dry in winter. Therefore, the choice of local greening ground cover materials is relatively limited. Beijing Green Township land is. Relevant researchers conducted field investigations on various garden green spaces in counties, districts and urban areas in Beijing and found that the plants of the genus Villingensis showed good ecological benefits and landscape effects in the application of gardens, but the plants of the genus Villingia were relatively simple. , mainly three kinds of stolon vegetables, goose down and berry vegetables. Since 2016, Zhao Fan began to investigate the resources of the genus genus genus genus genus genus in Beijin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/005
Inventor 董丽张浩然范舒欣郝培尧赵琳郑亦卿张启翔程堂仁王佳
Owner BEIJING FORESTRY UNIVERSITY
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