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Application of trichoderma koningii SMF2 in prevention and treatment of plant bacterial diseases

A trichoderma pseudocorning, bacterial technology, applied in the field of plant disease biological control, can solve problems such as lack of application of plant bacterial diseases, achieve significant control effects, reduce residues, and reduce dosage effects

Pending Publication Date: 2022-07-15
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing new biological control strategies, Trichoderma is mainly used to control plant fungal diseases, especially the application of control plant bacterial diseases is lacking.

Method used

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  • Application of trichoderma koningii SMF2 in prevention and treatment of plant bacterial diseases
  • Application of trichoderma koningii SMF2 in prevention and treatment of plant bacterial diseases
  • Application of trichoderma koningii SMF2 in prevention and treatment of plant bacterial diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Cultivation of Trichoderma pseudocorning SMF2

[0043] The culturing method of Trichoderma pseudocanning SMF2 strain, the concrete steps are as follows:

[0044] (1) inoculate the Trichoderma quaternum SMF2 strain with the strain preservation number of CCTCC NO: M209031 on the PDA solid medium, and cultivate at 28° C. for 48 hours to obtain the activated Trichoderma quasicornis SMF2;

[0045] (2) The activated Trichoderma quasiformis SMF2 was transferred into a PDA liquid medium, and cultured at 28° C. and 180 rpm for 12 days to obtain a Trichoderma quasiformis SMF2 seed culture solution.

[0046] The components of the PDA solid medium are as follows: 200 parts of potatoes, 20 parts of glucose, 1000 parts of ionized water, and 1.5 parts of agarose, all of which are parts by weight. The components of the PDA liquid culture medium are as follows: 200 parts of potatoes, 20 parts of glucose, and 1000 parts of ionized water, all of which are parts by weight.

Embodiment 2

[0047] Example 2 Trichoderma pseudocanning SMF2 inhibits the growth of Xoo and Xoc

[0048] The activated Trichoderma quasicornin SMF2 in Example 1 was transferred to the NB solid medium containing Xoo and the NB solid medium containing Xoc (OD of Xoo and Xoc) respectively. 600 Both were 0.01), cultured at 28°C for 6 days, to observe the situation that Trichoderma pseudocorning SMF2 produced a bacteriostatic zone on Xoo or Xoc, and the NB solid medium containing only Xoo or Xoc was used as a control group. The results are as follows figure 1 shown.

[0049] The activated Trichoderma pseudocanning SMF2 in Example 1 was transferred to the NB liquid medium containing Xoo and the NB solid medium containing Xoc (OD of Xoo and Xoc) 600 Both were 0.01), cultured at 28°C and 180rpm for 48h, and the number of Xoo or Xoc cells was determined. The NB liquid medium containing only Xoo or Xoc was used as the control group. The results are as follows figure 2 shown.

[0050] The compone...

Embodiment 3

[0054] Example 3 Extraction of Trichoderma pseudocanning SMF2 secondary metabolite kangningmycin

[0055] The extraction method of the secondary metabolite kangningmycin of Trichoderma pseudocanning SMF2 is as follows:

[0056] a, liquid extraction method

[0057] (1) by the Trichoderma pseudocorning SMF2 seed culture liquid among the embodiment 1, filter through three layers of lens paper, remove mycelium, keep filtrate;

[0058] (2) centrifuge the filtrate at 10000rpm for 10min, and get the supernatant;

[0059] (3) Take 20 mL of the centrifuged supernatant, add it to the SPE C18 adsorption column, and stand to filter;

[0060] (4) Add 2 mL of methanol solution to the SPE C18 adsorption column to dissolve the adsorbed substances, and finally filter the solution through a 0.22 μm organic filter to obtain a crude extract of metabolites, which is stored at 4°C for later use.

[0061] b. Solid extraction method

[0062] (1) Using a circular hole punch with a diameter of 0.5 ...

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Abstract

The invention relates to application of trichoderma koningiopsis SMF2 in prevention and treatment of plant bacterial diseases. The preservation number of the trichoderma koningiopsis SMF2 is CCTCC (China Center For Type Culture Collection) NO: M209031. The invention also provides an application of the secondary metabolite kangning mycin of the trichoderma koningii SMF2 in preventing and treating plant bacterial diseases, and a preparation method of the kangning mycin. The trichoderma koningii SMF2 disclosed by the invention is an important biocontrol trichoderma, a secondary metabolite Kanning mycin can be efficiently synthesized, and the Kanning mycin has a remarkable antagonistic effect on various pathogenic fungi and gram-positive pathogenic bacteria and can effectively prevent and treat plant diseases caused by the bacteria. Particularly, the compound can effectively inhibit the growth of xanthomonas oryzae pv. Oryzae and xanthomonas oryzae pv. Oryzae, and has remarkable prevention and treatment effects on rice bacterial blight and rice bacterial streak caused by xanthomonas oryzae pv. Oryzae pv. Oryzae pv. Oryzae pv. Oryzae pv. Oryzae.

Description

technical field [0001] The invention relates to the application of Trichoderma pseudocorning SMF2 in preventing and controlling bacterial diseases of plants, and belongs to the technical field of biological control of plant diseases. Background technique [0002] Bacterial diseases of plants are one of the important constraints on high quality and high yield of crops, causing direct economic losses of hundreds of billions of dollars every year. Chemical pesticide control and crop breeding are important means to control plant bacterial diseases. However, due to the limitations of environmental pollution caused by chemical pesticides and the long cycle of crop breeding, the establishment of environmentally friendly biological control technology has become the focus of research. Microbial biological control technology has the characteristics of high efficiency, no pollution and strong soil determination ability, which meets the requirements of sustainable agricultural developme...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/38A01P1/00C12R1/885
CPCC12N1/14A01N63/38
Inventor 张玉忠张玉强宋晓妍陈秀兰
Owner SHANDONG UNIV
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