Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus rhamnosus CCFM1252 capable of producing sulforaphane through metabolism to relieve inflammatory response

A technology of CCFM1252 and Lactobacillus rhamnosus, which is applied in the field of microorganisms to achieve the effects of regulating transcription levels, reducing colonic oxidative stress levels, and reducing release

Active Publication Date: 2022-07-15
JIANGNAN UNIV
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently, there is no strain that can metabolize sulforaphane to reduce inflammation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus rhamnosus CCFM1252 capable of producing sulforaphane through metabolism to relieve inflammatory response
  • Lactobacillus rhamnosus CCFM1252 capable of producing sulforaphane through metabolism to relieve inflammatory response
  • Lactobacillus rhamnosus CCFM1252 capable of producing sulforaphane through metabolism to relieve inflammatory response

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1: Screening, identification, observation and preservation of Lactobacillus rhamnosus CCFM1252.

[0068] Take 0.5 g of fresh fecal samples from healthy adults and add them to 4.5 mL of 0.9% normal saline for gradient dilution. Select the appropriate gradient dilution and spread it in MRS solid modified medium supplemented with 0.2% bromocresol purple. Incubate under anaerobic conditions at 37°C for 24-48h. A single colony with an obvious discoloration circle was selected and inoculated onto an MRS plate for streak purification, and a single colony was picked and transferred to a liquid MRS liquid medium for enrichment, and stored in 30% glycerol to obtain the strain Lactobacillus rhamnosus CCFM1252.

[0069] The whole genome DNA of strain CCFM1252 was extracted for the amplification of 16S rDNA, and the amplified DNA fragments were collected and sequenced (completed by Suzhou Jinweizhi Biotechnology Co., Ltd.), and the sequence was compared in NCBI. The strain ...

Embodiment 2

[0071] Example 2: Growth of Lactobacillus rhamnosus CCFM1252 in glucoraphanin-containing medium

[0072] The cryopreserved rhamnosus CCFM1252 was streaked in MRS solid medium, cultured anaerobic at 37°C for 24 to 48 hours, and then passaged through MRS liquid medium for 2 to 3 times, with an inoculum of 2% to 4%. Inoculated in the modified MRS liquid medium, cultured in an anaerobic environment at 37°C, and the OD of the culture medium was measured every 2h. 600 value, and retained the supernatant for HPLC detection of glucoraphanin content. When the growth of rhamnose CCFM1252 entered a stable phase, the measurement was stopped.

[0073] The experimental results are as figure 2 shown, Lactobacillus rhamnosus CCFM1252 in the modified MRS medium containing glucoraphanin, the OD 600 The value increases continuously and enters a stable period around 10h. The content of glucoraphanin was detected by HPLC, and it was found that the content of glucoraphanin continued to decreas...

Embodiment 3

[0074] Example 3: Determination of Lactobacillus rhamnosus CCFM1252 metabolic sulforaphane activity

[0075]The cryopreserved rhamnosus CCFM1252 was streaked in MRS solid medium, cultured anaerobic at 37°C for 24 to 48 hours, reactivated 2 to 3 times, and then inoculated into MRS liquid culture with 2% to 4% of the inoculum. After culturing in the anaerobic environment at 37°C for 24 hours, take 1 mL of bacterial solution and centrifuge to collect the bacterial slurry, resuspend the bacterial slurry with 0.05 mM phosphate buffer solution (pH=7.0), and crush it with 45 Hz ultrasonic, and then centrifuge to obtain Cell disruption supernatant. In a 1.5 mL centrifuge tube, add 100 μL glucoraphanin with a concentration of 1 mM, 600 μL cell disruption supernatant, and 100 μL 1 mM ascorbic acid, wherein both glucoraphanin and ascorbic acid are dissolved in phosphate buffer. After 150 min of reaction at 37°C, the supernatant was retained for the determination of sulforaphane content ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses lactobacillus rhamnosus CCFM1252 capable of producing sulforaphane through metabolism to relieve inflammatory response, and belongs to the technical field of microorganisms. The invention provides a strain of Lactobacillus rhamnosus CCFM1252 which has relatively strong glucoraphanin metabolism capability and can biologically convert sulforaphane, the Lactobacillus rhamnosus CCFM1252 provided by the invention can be used for improving ulcerative colitis and / or systemic inflammation symptoms independently or in combination with sulforaphane, can relieve weight loss during ulcerative colitis, and can be used for treating ulcerative colitis and / or systemic inflammation symptoms. Mucosal injury, reduction of the oxidative stress level of colon, regulation of the transcription level of colon proinflammatory factors, and reduction of release of the proinflammatory factors; and the polypeptide has the potential of reducing the oxidative stress level, reducing the release of proinflammatory factors and relieving the injury of colon, liver and the like.

Description

technical field [0001] The invention relates to a strain of Lactobacillus rhamnosus CCFM1252 that metabolizes and produces sulforaphane to reduce inflammation, and belongs to the technical field of microorganisms. Background technique [0002] Sulforaphane is an isothiocyanate, which can be obtained by the hydrolysis of glucoraphanin by myrosinase, the molecular formula is C 6 H 11 NOS 2 . Sulforaphane does not normally occur naturally in plants, but in cruciferous plants in the form of precursor glucoraphanin, and when plant tissue is damaged, glucoraphanin reacts with myrosinase to produce sulforaphane. However, sulforaphane itself is unstable, volatile, and easily decomposed under high temperature and alkaline conditions. Therefore, there are certain difficulties in the extraction of sulforaphane. [0003] Sulforaphane has received extensive attention due to its anti-inflammatory, anti-cancer, antioxidant and other effects. Studies have shown that sulforaphane can in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/00A61K35/747A61K31/70A61P1/04A61P29/00A23L33/105A23L33/135C12R1/225
CPCC12N1/20C12P13/00A61K35/747A61K31/70A61P1/04A61P29/00A23L33/135A23L33/105A23V2002/00A61K2300/00A23V2200/30A23V2200/32A23V2200/3204A23V2200/21Y02A50/30
Inventor 崔树茂邬佳颖毛丙永唐鑫张秋香赵建新陈卫
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com