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Detection method of agastache rugosus and preparation thereof

A detection method and preparation technology, applied in the field of medicine, can solve problems such as the quality of Difficult Agricola and its preparations, and achieve the effects of convenient analysis, improved scientificity and high precision

Pending Publication Date: 2022-07-15
SICHUAN NEO GREEN PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, it is difficult to effectively compare and analyze the differences and changes of Huoxiang and its preparations, and it is difficult to evaluate and control the quality of Huoxiang and its preparations as a whole

Method used

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  • Detection method of agastache rugosus and preparation thereof
  • Detection method of agastache rugosus and preparation thereof
  • Detection method of agastache rugosus and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The HPLC detection method of Huoxiang medicinal materials includes the following steps:

[0058] 1. Chromatographic conditions and system suitability test:

[0059] 1.1 Preparation of the test solution: take about 0.5 g of Huoxiang crude powder, accurately weigh it, put it in a conical flask with a stopper, add 25 mL of methanol, use ultrasonic treatment (power 500W, frequency 40kHz) for 30min, let it cool, Filter, take the filtrate to get the test solution.

[0060] Preparation of reference solution: Take appropriate amounts of caffeic acid, terinoside and acacia locust respectively, accurately weigh them, and use methanol to prepare reference solution with a concentration of 40 μg / mL.

[0061] Precisely draw 10 μL of the reference solution and the test solution, respectively, and inject them into the liquid chromatograph for determination and analysis.

[0062] 1.2 HPLC analysis chromatographic conditions: use octadecylsilane-bonded silica gel as filler (column leng...

Embodiment 2

[0127] The HPLC detection method of Huoxiang standard decoction includes the following steps:

[0128] 1. Chromatographic conditions and system suitability test:

[0129]According to the method of Example 1, the chromatographic conditions and system adaptability test were carried out, in which the Huoxiang medicinal material was replaced with the freeze-dried powder of the Huoxiang standard decoction. The results showed that the HPLC analysis chromatographic conditions of the Huoxiang standard decoction were: Silane-bonded silica gel is used as filler (column length is 250 mm, inner diameter is 4.5 mm, particle size is 5.0 μm); acetonitrile is used as mobile phase A, and 1.0% acetic acid is used as mobile phase B, and the gradient is carried out according to Table 1 in Example 1. Elution, the detection wavelength is 330 nm, the flow rate is 1.0 mL / min, and the column temperature is 35 °C.

[0130] 2. Preparation and investigation of the test solution:

[0131] 2.1 Investigat...

Embodiment 3

[0152] The HPLC detection method of Huoxiang Formula Granules includes the following steps:

[0153] 1. Chromatographic conditions and system suitability test:

[0154] The chromatographic conditions and system adaptability test were carried out according to the method of Example 1, wherein the Huoxiang medicinal material was replaced with Huoxiang formula granules. The results showed that the HPLC analysis chromatographic conditions of the obtained Huoxiang formula granules were as follows: binding silica gel with octadecylsilane It is a filler (the column length is 250 mm, the inner diameter is 4.5 mm, and the particle size is 5.0 μm); acetonitrile is used as mobile phase A, 1.0% acetic acid is used as mobile phase B, and gradient elution is carried out according to Table 1 in Example 1, and the detection wavelength is 330 nm, the flow rate is 1.0 mL / min, and the column temperature is 35 °C.

[0155] 2. Preparation and investigation of the test solution:

[0156] Take 0.1 ...

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Abstract

The invention provides a method for detecting wrinkled gianthyssop herb and a preparation thereof, which is characterized by comprising the following step: detecting caffeic acid, tilianin and acacetin in the wrinkled gianthyssop herb and the preparation thereof by adopting a high performance liquid chromatography, the chromatographic conditions of the high performance liquid chromatography are as follows: methanol is taken as a mobile phase A, one of 1% acetic acid, 1% phosphoric acid and 1% formic acid solution is taken as a mobile phase B, and gradient elution is carried out. The method has better precision and repeatability, is strong in operability, and can realize quality control of the agastache rugosus and the preparation of the agastache rugosus. The scientificity and rationality of overall evaluation of the related technological process of agastache rugosus are improved, the inherent quality of agastache rugosus and preparations thereof can be controlled integrally, and the clinical curative effect of the preparations such as agastache rugosus formula granules is ensured.

Description

technical field [0001] The invention belongs to the technical field of medicine, and particularly relates to a detection method of Huoxiang and its preparation. Background technique [0002] Agastache rugosa, also known as Agastache rugosa (Fisch.et Mey.)O.Ktze. part. Huoxiang is an aromatic plant, widely distributed in Hunan, Henan, Zhejiang, Jiangsu, Jiangxi and Sichuan and other provinces. It has good nutritional value, medicinal value and economic value. Jing, has the effect of aromatizing turbidity, regulating qi and stomach, stopping vomiting, and releasing summer heat. It is often used to relieve or treat summer heat, headache, fever, chest tightness and abdominal distension, vomiting, diarrhea and other symptoms. [0003] In the prior art, the researches on Agaricus are mostly concentrated on the determination of chemical components and volatile oils. The research on the chemical components of Agaricus is mainly based on methyl piperonol and piperonyl methyl ether....

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679G01N2030/065
Inventor 周厚成周靖惟张晨张开元胡昌江胡庆张林黄宇姜艳娇梅国荣孙纪元费文波
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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