Method for enhancing microalgae carbon sequestration and oil production by using diethanol amine

A diethanolamine, microalgae technology, applied in microorganism-based methods, biochemical equipment and methods, with the addition of compounds to stimulate growth, etc., can solve the lack of stimulating lipid content, slow mass transfer, high oil content and It is difficult to obtain high oil yield at the same time, so as to achieve the effect of increasing the oil content of the cell, increasing the final yield, and promoting the metabolism of oil production.

Pending Publication Date: 2022-07-26
CHONGQING UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the researchers found that while nitrogen deficiency stimulated the increase of lipid content, it was often accompanied by stagnation of cell growth and reduction of biomass, which would lead to an ineffective increase in lipid yield.
Therefore, it is often difficult to obtain high oil content and high oil production ra

Method used

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  • Method for enhancing microalgae carbon sequestration and oil production by using diethanol amine
  • Method for enhancing microalgae carbon sequestration and oil production by using diethanol amine

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1

[0023] The method for enhancing carbon fixation and oil production of microalgae by using diethanolamine in this embodiment includes the following steps: adding 1 mg / L of diethanolamine to the BG11 medium, and then inoculating the Chlorella in the logarithmic growth phase according to the initial inoculation concentration of about 1 mg / L. The microalgae solution was prepared by inoculating 100mg / L in nutrient-rich BG11 medium. The initial pH value of the microalgae solution was 6.5, the culture temperature was 25°C, the light intensity was 4000Lux, and the light cycle was 12h:12h (light: dark) , 3% CO was introduced into the microalgae liquid culture 2 , the air supply rate was 20mL / min and the aeration stone was used for aeration, which lasted for 12h, and the culture period was 14 days. During the culture process, the biomass of Chlorella was measured every day.

Example Embodiment

[0024] Embodiment 2

[0025] The method for enhancing carbon fixation and oil production of microalgae by using diethanolamine in this embodiment includes the following steps: adding 5 mg / L of diethanolamine to the SE medium, and then chlorella in logarithmic growth phase according to the initial inoculation concentration of 100mg / L was inoculated into nutrient-rich SE medium to obtain microalgae solution. The initial pH value of the microalgae solution was 7.5, the culture temperature was 35°C, the light intensity was 4000Lux, and the light cycle was 12h:12h (light: dark), Bubble 15% CO during microalgae culture 2 , the air supply rate was 12mL / min and the aeration stone was used for aeration, which lasted for 2h, and the culture period was 14 days. During the culture process, the biomass of Chlorella was measured every day.

Example Embodiment

[0026] Embodiment 3

[0027] The method for enhancing carbon fixation and oil production of microalgae by using diethanolamine in this embodiment includes the following steps: adding 2 mg / L of diethanolamine to SE medium, and then setting the initial inoculation concentration of Scenedesmus in logarithmic growth phase to 200 mg according to the initial inoculation concentration. / L was inoculated into nutrient-rich SE medium to obtain microalgae solution. The initial pH value of the microalgae solution was 7.5, the culture temperature was 35°C, the light intensity was 6000 lux, and the light cycle was 12h:12h (light: dark). Introduce 15% CO during microalgae culture 2 , the air supply rate was 45mL / min and the aeration stone was used for aeration, which lasted for 12h, and the culture period was 14 days. During the culture process, the biomass of Scenedesmus was measured every day.

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Abstract

The invention discloses a method for enhancing microalgae carbon sequestration and oil production by using diethanol amine. The method comprises the following steps: introducing CO2 into a microalgae culture system, and adding diethanol amine to enhance CO2 gas dissolution and mass transfer and stress microalgae oil production; by adding diethanol amine, the capacity of the algae liquid for storing carbon dioxide is firstly improved, and then the mass transfer process of dissolving carbon dioxide in the algae liquid from air is accelerated, so that the photosynthesis of microalgae is favorably improved, and the increase of microalgae biomass is accelerated; in addition, oil production metabolism can be promoted through stress of diethanol amine on microalgae cells, so that the oil content of the cells is increased. Through promotion of biomass and cell oil content, the final yield (oil yield = biomass * cell oil content) of microalgae oil is improved.

Description

technical field [0001] The invention relates to the technical field of microalgae biology, in particular to a method for enhancing carbon fixation and oil production of microalgae by using diethanolamine. Background technique [0002] Under normal circumstances, microalgae can obtain relatively fast growth rates when cultured under full nutrient conditions, but the oil content is not high. A large number of studies have shown that unfavorable growth environmental factors can increase the lipid content of microalgae, such as nitrogen deficiency, silicon deficiency, phosphorus limitation, heavy metal stress, high light and high temperature stress, hormone effects, etc. Among them, the effect of nitrogen deficiency is the most significant. , and is considered to be the most effective way to stimulate the increase of oil content. However, the researchers found that nitrogen deficiency stimulated the increase of lipid content, which was often accompanied by stagnation of cell gr...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/38C12P7/6436C12R1/89
CPCC12N1/12C12N1/38C12P7/6436
Inventor 聂煜东沈倩
Owner CHONGQING UNIV OF TECH
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