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SNP (Single Nucleotide Polymorphism) site combination related to net hair rate of fine-wool sheep and application of SNP site combination

A technology for fine wool and net wool ratio, which is applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc. It can solve the problems affecting the economic value of wool, low net wool ratio of fine wool sheep, etc., and shorten the generation interval. , low cost and wide applicability

Active Publication Date: 2022-07-26
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the coat of fine-wool sheep is homogeneous fine hair composed entirely of unmyelinated fine hair, the fineness is less than 60, that is, the wool fiber diameter is below 25 microns, so the net wool rate of fine-wool sheep is generally low, which seriously affects Economic value of wool
However, there are few studies on the net wool rate of fine-wool sheep at present, so the present invention aims to use the widely used molecular genetic marker method to explore the genetic traits related to the net wool rate of fine-wool sheep

Method used

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  • SNP (Single Nucleotide Polymorphism) site combination related to net hair rate of fine-wool sheep and application of SNP site combination
  • SNP (Single Nucleotide Polymorphism) site combination related to net hair rate of fine-wool sheep and application of SNP site combination
  • SNP (Single Nucleotide Polymorphism) site combination related to net hair rate of fine-wool sheep and application of SNP site combination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 SNP sites related to the net wool ratio of fine wool wool

[0034] 1. Obtaining the total SNP set

[0035] Whole-genome resequencing was performed on 460 fine-wool sheep individuals in four representative fine-wool sheep breeds in my country, with an average depth of 5X, using the resequencing analysis process, and the sheep v4.0 reference genome released in 2015 (from NCBI). The common results obtained by comparing the two methods form a SNP set.

[0036] Specifically, the high-depth resequencing of multiple fine-wool sheep individuals is completed by a biosequencing company, and the sequencing results completed by the biosequencing company can achieve the technical purpose of the present invention, which is not limited in the present invention. In this application, the Fastq file returned by the sequencing company is compared to the reference genome sheep v4.0 to obtain the BAM file, and the VCF file containing the population SNP typing information is obtain...

Embodiment 2

[0046] Example 2 Panel preparation of SNPs related to the net wool ratio of fine-wool wool

[0047] Based on the combination of SNP sites obtained in Example 1, this application entrusts Boruidi Biotechnology Co., Ltd. to prepare panels of SNPs related to the net gross rate. Add multiple PCR Panel mix and multiple PCR amplification enzyme system to the DNA that has passed the quantitative quality inspection, and place it on the PCR machine to complete the PCR reaction. After the PCR product was purified by carboxyl magnetic beads, it was counted into the sequencing primer with Barcode and the high-fidelity PCR reaction system for PCR amplification. Different Barcodes were used to distinguish different samples. After purification by carboxyl magnetic beads, the amplification product is completed, and the multiplex PCR capture and library construction are completed. Those skilled in the art design primers according to the sequence information of each locus in the SNP locus comb...

Embodiment 343

[0048] Example 3 Detection of individual net wool ratio of 437 fine wool sheep

[0049] Based on the SNP site combination obtained in Example 1 and the panel preparation in Example 2, individuals of fine-wool sheep were detected. In one embodiment of the present invention, GenoBaits (based on Targeted gene capture technology scheme for hybridization) to detect the wool net wool rate of its individual. The working principle of this technology is based on the complementary combination of the target probe and the target sequence for site-specific capture, elution of the captured target sequence, target amplification, library construction and sequencing, and finally the genotype of the target SNP is obtained. Under effective conditions, the number of target sites and their labels that can be detected is equivalent to that of high-density solid-phase chips in terms of detection density and throughput. Through this technique, the result value of the target sample is obtained. Tabl...

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Abstract

The invention belongs to the technical field of biology, and relates to an SNP (Single Nucleotide Polymorphism) site combination related to the net hair rate of fine-wool sheep and application. The invention provides a combination of 11 SNP loci related to the net wool rate of fine-wool sheep. The SNP loci are determined based on sheep v4.0 genome sequence comparison; the invention provides application of a reagent for detecting the genotype of a combination of 11 SNP loci related to the net wool rate of fine-wool sheep in a fine-wool sheep genome in detection of the net wool rate of fine-wool sheep or molecular marker-assisted breeding of fine-wool sheep. According to the present invention, the site combination, the molecular probe combination formed based on the site combination, the gene chip and the kit can be used to analyze the pure wool rate character of the individual fine wool sheep, the pure wool rate character difficult to measure in the early stage is subjected to individual selection, the generation interval is shortened, the breeding process is accelerated, and the breeding efficiency is improved. A large amount of breeding cost is saved, and support is provided for identification, protection and molecular genetic breeding of the fine-wool sheep in the future.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a combination and application of SNP sites related to the net wool ratio of fine-wool sheep. Background technique [0002] Sheep (Ovis aries), as one of the first animals to be domesticated, have played an important role in human society and have multiplied almost globally through human migration. Humans domesticate sheep for feeding, mainly to meet the survival needs of human society, especially for people living in remote and nomadic areas. With the improvement of people's living standards and the continuous enrichment of material needs, fine-wool sheep have become one of the economic production performance categories of sheep due to the production of secondary products such as wool, and the specialization of their cultivation has emerged. [0003] Fine-wool sheep are known for their production of high-quality wool, a natural fiber that is an important agricultural pro...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124Y02P60/87
Inventor 郭婷婷袁超刘建斌岳耀敬卢曾奎牛春娥李建烨孙晓萍马炳洁杨博辉
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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