Application of 2-amino-3-phenylbutyric acid or derivative thereof as plant growth regulator
A plant growth regulator, phenylbutyric acid technology, applied in the application field of plant growth regulators, to achieve the effect of simple structure, promotion of growth and development, and low dosage
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Embodiment 1
[0041] Example 1: Effect of 2-amino-3-phenylbutyric acid on rooting of cucumber seedlings
[0042] In order to study the effect of 2-amino-3-phenylbutyric acid on plant root growth, a model system for studying root development, the radicle-depleted cucumber seedling system was selected. Spread four layers of moist gauze on the bottom of the culture box, select cucumber seeds with full shape and uniform size (the variety is "early summer and autumn crown", Shandong Ningyang County Luming Seed Co., Ltd.), disinfect with 75% alcohol, rinse with distilled water After cleaning, spread it evenly on the gauze, and then cover it with a damp four-layer gauze to avoid light. After 24 hours in a 25°C culture room protected from light, the germinated cucumber seeds were taken out and buried 1 centimeter (cm) below the soil. After 3 days, take a number of seedlings with the same growth and fully expanded two cotyledons, rinse the roots with distilled water, cut off the radicles along the ...
Embodiment 2
[0047] Example 2: The effect of 2-amino-3-phenylbutyric acid on the growth of cucumber seedlings
[0048] In order to study the effect of 2-amino-3-phenylbutyric acid on the growth of plants, four layers of moist gauze were spread on the bottom of the culture box, and cucumber seeds with full shape and uniform size were selected (the variety is "early summer and autumn crown", Shandong Ningyang County Luming Seed Co., Ltd.), sterilized with 75% alcohol, rinsed with distilled water, spread it evenly on the gauze, and then covered it with a moist four-layer gauze to avoid light. After 24 hours in a 25°C culture room, darkened from light, the germinated cucumber seeds were taken out and buried in plastic pots (12cm in diameter) filled with moist (saturated water) soil, with a depth of 2-3cm, 5 seeds per pot, and placed in 25 ℃ culture room (light intensity 200 μmol m -2 s -1 )nourish. After 3 days, 1 robust seedling with fully expanded cotyledons was kept, and the other 4 seed...
Embodiment 3
[0053] Example 3 Effect of 2-amino-3-phenylbutyric acid seed soaking treatment on rice plant growth
[0054] Rinse the rice seeds with distilled water, disinfect with 75% alcohol for 3 minutes, wash with distilled water for 3 times, then disinfect the rice seeds with 5% NaClO aqueous solution for 10 minutes, take them out and rinse with distilled water, and then use a clean filter paper to absorb the seeds Residual moisture on the surface until the surface is dry. Pick 50 g of healthy, plump and uniform seeds and place them in a 150 mL conical flask. Add 100 mL of 2-amino-3-phenylbutyric acid solution with concentrations of 0, 10, 100, and 1000 nM to each bottle, soak them at room temperature for 5 days, and take them out. Select 100 rice seeds for the test with the same germination state, sow them evenly in the rice seedling tray, cover with soil, and place the tray in a 28°C culture room. When the rice seedlings have 3 leaves and 1 center, the plant height and Root length a...
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