Human adipose tissue acellular extracellular matrix as well as preparation and application thereof

A fat tissue and decellularization technology, applied in medical science, prosthesis, etc., can solve the problems of protein content and structural integrity, long preparation steps, low adipogenesis efficiency, etc., to improve preparation efficiency and output, and wide application The effect of foreground, operation simplification

Pending Publication Date: 2022-07-29
JINAN UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

This method takes a long time to prepare, and adipose tissue has to undergo digestion with isopropanol and biological enzymes during the preparation process, which poses a challenge to the biocompatibility and safety of DAT
In addition, because adipose tissue has undergone

Method used

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  • Human adipose tissue acellular extracellular matrix as well as preparation and application thereof
  • Human adipose tissue acellular extracellular matrix as well as preparation and application thereof
  • Human adipose tissue acellular extracellular matrix as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0058] Example 1 Preparation of human adipose tissue decellularized extracellular matrix by an improved decellularization method

[0059] 1. First prepare granular fat into highly concentrated nano fat, such as figure 1 shown:

[0060] ①Preliminary centrifugation to remove water: Particulate fat (obtained from healthy patients (19-45 years old) who underwent autologous fat transplantation in the First Affiliated Hospital of Jinan University from October 2016 to October 2018. Approved by the medical ethics committee of a hospital) placed in a sterile bucket containing ice, centrifuged at 1000g × 3min to remove the liquid layer; this step is to remove the streak-like fat structure that may exist in the granular fat;

[0061] ② Cut fat cells: According to the standard of Tonnard et al. 2013, the fat obtained in step ① was cut 30 times with double nut connectors with spacings of 2.4mm, 1.2mm and 1.0mm, respectively, and then filtered with a 500-mesh sieve;

[0062] ③ Negative pr...

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Abstract

The invention discloses a human adipose tissue acellular extracellular matrix as well as preparation and application thereof, and relates to the technical field of biomedical materials. The method comprises the following steps: S1, preparing concentrated nano fat; s2, thoroughly crushing fat cells; and S3, removing the chemical reagent. Compared with a traditional method, the principle is that the contact time of chemical and biological reagents is shortened, and the physical treatment time is prolonged. Compared with a traditional method, the method has the advantages that the preparation time is greatly shortened, the operation is simplified, and particularly, the treatment of isopropanol and pancreatin is completely omitted. The obtained extracellular matrix retains more types of proteins, more types of proteins promoting lipogenesis are achieved, the middle and long-term vascularization and lipogenesis capacities after transplantation are higher than those of a traditional method, and the method has wide application prospects in experimental research and clinical treatment in the future.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a human adipose tissue decellularized extracellular matrix and its preparation and application. Background technique [0002] Adipose tissue decellularized adipose tissue (DAT) has the advantages of relatively abundant sources, no immune rejection, and natural extracellular matrix structure compared with synthetic matrix materials. It can be used as a matrix material for stem cells, cytokines, etc., to enhance the repair efficiency, and has a wide range of application prospects, which has become an important direction for the development of matrix materials at present. However, how to improve the repair effect of acellular matrix materials and minimize possible unsafe factors is a key scientific problem to be solved in the preparation of acellular adipose tissue. [0003] There are various DAT extraction methods, but they all lack a unified consensus. Pure physical...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3633A61L27/3604A61L27/3691A61L27/3687A61L27/50A61L2400/06A61L2430/40
Inventor 蒋笑刘宏伟唐灵芝黄红茵赖芯蕊李泽华吴引弟李升红廖选
Owner JINAN UNIVERSITY
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