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IncRNA related to chicken sexual maturity or heterosis, target gene regulated by lncRNA and application of lncRNA

A hybrid vigor, mature technology, applied in the direction of DNA/RNA fragments, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problem of LncRNA without literature reports

Pending Publication Date: 2022-07-29
INST OF ANIMAL SCI CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Studies have shown that lncRNAs play an important role in the regulation of gonad development and sexual maturation in livestock and poultry, but there are no relevant lncRNAs that can be used to evaluate chicken sexual maturity and its heterosis.

Method used

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  • IncRNA related to chicken sexual maturity or heterosis, target gene regulated by lncRNA and application of lncRNA
  • IncRNA related to chicken sexual maturity or heterosis, target gene regulated by lncRNA and application of lncRNA
  • IncRNA related to chicken sexual maturity or heterosis, target gene regulated by lncRNA and application of lncRNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Screening of molecular markers related to chicken sexual maturity

[0056] ⒈ Test method

[0057] ⑴ group building

[0058] Choose White Leghorn Chicken (WW) and Beijing Oil Chicken (YY), and perform pure breeding and positive and negative crosses according to the ratio of male to female to form 2 pure breeding combinations (WW, YY) and 2 hybrid combinations (WY, YW) ). The chickens are raised in the same house with the same management method.

[0059] (2) Phenotypic analysis and sample collection

[0060] When the chickens were 22 weeks old, 6 individuals were selected from the WW, YY, WY, and YW combinations according to the individual pubic width for ovarian tissue sample collection and fallopian tube length measurement, and the ovarian tissue was put into liquid nitrogen for preservation, and then transferred to - 80 ℃ refrigerator for spare use, the entire sampling process to maintain a sterile environment. At the same time, the blood of the selected...

Embodiment 2

[0063] Example 2 LncRNA and its target gene regulatory relationship and associated signaling pathway analysis

[0064] ⒈ Library sequencing

[0065] Sample rRNA was removed using the Epicentre Ribo-ZeroTM kit. Fragmentation is then performed, and first-strand cDNA is synthesized using random primers and reverse transcriptase, and then double-strand cDNA is synthesized. The purified double-stranded cDNA is then subjected to end repair, A is added, and a sequencing adapter is connected, and finally a cDNA library is obtained by PCR amplification. After passing the quality inspection, the library was sequenced using an Illumina HiSeq sequencer to obtain the original sequencing data.

[0066] ⒉ LncRNA Screening

[0067] Clean reads were obtained from raw sequencing data after quality control. Taking the Gallus-gallus version 6.0 genome as the reference genome, and aligning and assembling transcripts according to the Hisat2-Stringtie process, LncRNA prediction was performed. F...

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Abstract

The invention discloses lncRNA related to chicken sexual maturity or heterosis, a target gene regulated by the lncRNA and application of the lncRNA. According to the invention, LncRNA significantly related to the heterosis of chicken first laying age, pubis width and fallopian tube length is screened through combined analysis of a high-throughput sequencing technology and different hybrid combination ovarian tissues LncRNA-mRNA, CACNA1C is preliminarily determined as a target gene of the CACNA1C, and the nucleotide sequence of the LncRNA is as shown in SEQ ID No.1. Experimental verification proves that the LncRNA affects GnRH secretion of an organism by up-regulating CACNA1C expression and regulates chicken sexual maturity. The high-expression LncRNA and the target gene thereof in the ovarian tissue are successfully screened, can be used as a molecular marker or target of sexual maturity and heterosis, are applied to prediction and breeding of chicken sexual maturity and heterosis and are beneficial to further clarification of a molecular mechanism of chicken sexual maturity.

Description

technical field [0001] The invention relates to long-chain non-coding RNAs (lncRNAs) related to chicken sexual maturity or heterosis and their regulated target genes, and the invention further relates to their application as molecular markers for chicken sexual maturity or heterosis prediction, belonging to the long chain Noncoding RNAs and their regulated target genes and application areas. Background technique [0002] In poultry production, sexual maturity is a trait with important economic value, and suitable sexual maturity time is very important for poultry egg production and egg quality. The sexual maturation of laying hens is usually regulated by the hypothalamic-pituitary-ovarian gonadal axis, and the regulation of gonadotropin-releasing hormone (GnRH) secretion is the basis of animal sexual maturation. The hypothalamus acts on the pituitary gland cells through the pituitary portal system by secreting GnRH, and promotes the secretion of the gonadotropins follicle-s...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/686C12N15/113
CPCC12Q1/6888C12Q1/686C12N15/113C12Q2600/156C12Q2600/178C12Q2563/107C12Q2545/114
Inventor 袁经纬陈继兰孙研研李云雷麻慧王园美
Owner INST OF ANIMAL SCI CAAS
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