Preparation method and device of amphiphilic molecular layer

Abstract

The invention relates to a method and a device for preparing an amphiphilic molecular layer, the method comprises the following steps: providing a preparation device for preparing the amphiphilic molecular layer, arranging at least one micropore, a first channel and a second channel in the preparation device, enabling a solution to flow through the micropore, and respectively communicating the first end and the second end of the micropore with the first channel and the second channel, the cross section of the first channel is square or quasi-square; s2, a first polar solution is added into the second channel, so that the first polar solution enters at least one micropore; s3, a membrane solution and a second polar solution are sequentially added into the first channel, so that the membrane solution forms a meniscus in the first channel; s4, adding a second polar solution into the first channel based on a preset speed, so that the second polar solution pushes the membrane solution to move and flow through the micropores, and the membrane solution forms an amphiphilic molecular layer at the micropores; wherein the first channel material surface contact angle is from about 65 degrees to about 120 degrees. The method is simple to operate and relatively good in repeatability.

Description

technical field [0001] The invention relates to the field of amphiphilic molecular membranes, in particular to a preparation method and device for an amphiphilic molecular layer. Background technique [0002] Nanoporin needs to be embedded in a phospholipid or a polymer film, such as an amphiphilic molecular layer (that is, an amphiphilic molecular membrane), so that the DNA sequence to be detected can pass through, so as to detect the DNA sequence. the goal of. However, some current preparation devices and corresponding methods for preparing amphiphilic molecular layers still have some problems in application. [0003] The existing film-forming methods mainly include folded bilayer formation method (Montal & Mueller's method), dip-tip method, coating method, patch-clamp method and water-in-oil droplet interface method. The above methods such as folding double-layer method, dip tip method, coating double-layer method, etc., often form a thick film for the first time, and n...

AI Technical Summary

Problems solved by technology

However, the generation of bubbles often requires manual operation control (such as preparing bubbles through a pipette gun). This process is difficult to automate, and manual operation is difficult to control the size of the bubbles, and it is difficult to ensure that the shape of the bubbles is stable. Therefore, the repeatability of the method is also difficult. poor

And this method also requires preprocessing

[0005] Another example is the Chinese invention with application number CN200880126160.3, which discloses a method of forming a layer separating two volumes of aqueous solution by flowing an aqueous solution containing amphiphilic molecules through the body to cover the groove ( That is, micropores), so that the aqueous solution can cross the groove to form amphiphilic molecules, although the technology of making the aqueous solution flow through the groove to form amphiphilic molecules is easy to implement, but the prepared membrane is thick and needs subsequent thinning processing, and the method also involves a preprocessing step

[0006] In addition, there are also some problems in the existing preparation devices on the market. For example, in order to realize mass production and ensure the consistency of the tank body (that is, the flow channel for adding solution samples) (that is, to reduce production errors) ), the casting process (that is, through the mold) is often used to make the tank body. Therefore, in order to meet more experimental requirements, the internal structure of the tank body is usually set larger. Therefore, when some experiments only need a small amount of solution samples , will cause unnecessary waste of samples

And if the waste of samples is to be avoided, it is necessary to prepare sample tanks of different sizes according to different experimental requirements (different sequencing experiments require different volumes of tanks), and thus it is necessary to prepare corresponding molds for different sizes of sample tanks (also That is, each production needs to open the mold), which is costly in the industrial production process

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