Method for modifying and preparing humulus lupulus
A technology of modifying hops, applied in the field of health care products and food, to achieve the effect of mild reaction conditions, easy industrial production, and simple process
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Embodiment 1
[0023] (1) Disperse 100g of hops dry powder and 0.5g of cellulase in 500g of ethanol, ultrasonically disperse, and then keep the water bath at 50°C for 3h;
[0024] (2) Mix 0.1 g of cannabidiol, 0.05 g of polyethylene glycol, and 100 g of pure water, sonicate to disperse evenly, add the dispersion dropwise to the mixture in step (1), stir to mix evenly, and use sodium carbonate Adjust the pH of the mixture to 7;
[0025] (3) The mixture of step (2) is ultrasonically mixed at normal pressure in an ultrasonic mixer for 30min;
[0026] (4) Transfer the mixture in step (3) to a multi-effect external circulation evaporator, control the temperature at 60°C, and concentrate and dry under reduced pressure to obtain hops containing active cannabidiol.
[0027] The active cannabidiol hops prepared in this example were eluted isocratically with a chromatographic column: InertSustain C18 and methanol-water (volume ratio 85:15) as the mobile phase; under this chromatographic condition, ta...
Embodiment 2
[0029] (1) Disperse 200g of hops dry powder and 2g of cellulase in 500g of ethanol, ultrasonically disperse, and then keep the water bath at 60°C for 2h;
[0030] (2) Mix 2g of cannabidiol, 0.16g of polysorbate-80 and 200g of pure water, sonicate until dispersed evenly, add the dispersion dropwise to the mixture in step (1), stir to mix evenly, use hydrogen carbonate Sodium adjusts the pH of the mixture to 8;
[0031] (3) The mixture of step (2) is ultrasonically mixed at normal pressure in an ultrasonic mixer for 60min;
[0032] (4) Transfer the mixture in step (3) to a multi-effect external circulation evaporator, control the temperature at 50°C, and concentrate and dry under reduced pressure to obtain hops containing active cannabidiol.
[0033] The active cannabidiol hops prepared in this example were eluted isocratically with a chromatographic column: InertSustain C18 and methanol-water (85:15) as the mobile phase; The hop samples of active cannabidiol were injected and...
Embodiment 3
[0035] (1) Disperse 300g of hops dry powder and 6g of cellulase in 500g of ethanol, ultrasonically disperse, and then keep the water bath at 55°C for 2h;
[0036] (2) Mix 1.5 g of cannabidiol, 3 g of sodium dodecyl benzene sulfonate, and 300 g of pure water, sonicate until uniformly dispersed, drop the dispersion into the mixture in step (1), stir to mix uniformly, Adjust the pH of the mixture to 7 with sodium hydroxide;
[0037] (3) The mixture of step (2) is ultrasonically mixed at normal pressure in an ultrasonic mixer for 40min;
[0038] (4) Transfer the mixture in step (3) to a multi-effect external circulation evaporator, control the temperature at 60°C, and concentrate and dry under reduced pressure to obtain hops containing active cannabidiol.
[0039] The active cannabidiol hops prepared in this example were eluted isocratically with a chromatographic column: InertSustain C18 and methanol-water (85:15) as the mobile phase. Under this chromatographic condition, the C...
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