Fluorescent probe for detecting mycobacterium tuberculosis as well as preparation method and detection method thereof

A technology of Mycobacterium tuberculosis and fluorescent probe, applied in the field of molecular biology, can solve the problems of low sensitivity and low specificity, and achieve the effect of high sensitivity, strong specificity and high specificity

Pending Publication Date: 2022-08-02
THE THIRD PEOPLES HOSPITAL OF SHENZHEN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of this application is to provide a fluorescent probe for detecting Mycobacterium tuberculosis and its preparation method and detection method, aiming to solve the problems of low specificity and low sensitivity in the existing detection of Mycobacterium tuberculosis

Method used

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  • Fluorescent probe for detecting mycobacterium tuberculosis as well as preparation method and detection method thereof
  • Fluorescent probe for detecting mycobacterium tuberculosis as well as preparation method and detection method thereof
  • Fluorescent probe for detecting mycobacterium tuberculosis as well as preparation method and detection method thereof

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preparation example Construction

[0044] The second aspect of the embodiments of the present application provides a method for preparing a fluorescent probe for detecting Mycobacterium tuberculosis, such as figure 1 As shown, the preparation method comprises the following steps:

[0045] S11: Provides metabolic groups and aggregation-induced luminescent groups;

[0046] S12: Fluorescent probes are synthesized by mixing metabolic groups with aggregation-induced luminescent groups to undergo click chemistry reactions.

[0047] For the preparation method of the fluorescent probe provided in the examples of this application, by mixing the provided metabolic group with the aggregation-induced luminescent group, the click chemistry reaction is used to realize the efficient coupling reaction of the metabolic group and the aggregation-induced luminescent group, thereby synthesizing Fluorescent probe with the function of detecting Mycobacterium tuberculosis. In addition, the preparation method only uses the splicing ...

Embodiment A1

[0066] This embodiment provides a method for preparing a fluorescent probe. The preparation method comprises the following steps:

[0067] S11: provide 3-azido-D-alanine and 1-butyne-4-(2-(4-benzylaminophenylethene)pyridine bromide in a molar ratio of 1:0.2;

[0068] S12: Dissolve 3-azido-D-alanine and 1-butyne-4-(2-(4-dibenzylaminophenylethene)pyridinium bromide in dimethyl sulfoxide to obtain a mixed solution ; Disperse copper bromide in trimethylamine to form a dispersion; inject the dispersion into the mixture, stir at 25°C for 12 h, extract with dichloromethane, and centrifuge to obtain a solid containing fluorescent probes; dissolve the solid In dimethyl sulfoxide, filtered through a polyethersulfone membrane filter, and the collected organic layer was evaporated to dryness to obtain a fluorescent probe.

Embodiment A2

[0070] This embodiment provides a method for preparing a fluorescent probe.

[0071] The difference between this preparation method and Example A1 is that S11: according to the molar ratio of 1:0.34, 3-azido-D-alanine and 1-butyne-4-(2-(4-diphenyl) are provided methylaminophenyl vinyl) pyridinium bromide.

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Abstract

The invention relates to the technical field of molecular biology, and provides a fluorescent probe for detecting mycobacterium tuberculosis, the fluorescent probe comprises a metabolic group and an aggregation-induced emission group, the metabolic group is connected with the aggregation-induced emission group through a chemical bond, and the metabolic group is used for synthesizing mycobacterium tuberculosis cell wall peptidoglycan. According to the fluorescent probe provided by the invention, mycobacterium tuberculosis cell wall peptidoglycan is synthesized through the contained metabolic group, a large amount of mycobacterium tuberculosis cell wall peptidoglycan is gathered to the mycobacterium tuberculosis cell wall in a metabolic labeling manner, and the aggregation-induced emission group generates a fluorescence signal due to aggregation, so that the detection of mycobacterium tuberculosis is realized. In addition, the fluorescent probe can rapidly and conveniently detect mycobacterium tuberculosis, and is high in specificity and sensitivity.

Description

technical field [0001] The application belongs to the technical field of molecular biology, and in particular relates to a fluorescent probe for detecting Mycobacterium tuberculosis and a preparation method and detection method thereof. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis infection. Mycobacterium tuberculosis can invade various organs of the human body, but it mainly invades the lungs, which is called pulmonary tuberculosis. In 2020, 9.87 million people worldwide will be infected with tuberculosis and 1.28 million people will die from tuberculosis. Therefore, tuberculosis is a serious threat to human health and is a major public health problem. The global prevention and control of tuberculosis is still faced with drug resistance, lack of effective vaccines and early diagnosis methods. Therefore, timely and accurate detection of tuberculosis is crucial to the treatment and prevention of tuberculosis....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06C12Q1/04G01N21/64
CPCC09K11/06C09K11/025C12Q1/04G01N21/6428G01N21/6458C09K2211/1007C09K2211/1029C09K2211/1059C09K2211/1014G01N2021/6421
Inventor 赵鹏飞戴桂琴张政郑明彬廖明凤刘东京
Owner THE THIRD PEOPLES HOSPITAL OF SHENZHEN
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