5 results about "Mycobacterial cell" patented technology

The present disclose provides a primer, probe, and kit for broad-spectrum detection of mycobacteria and use thereof, relating to the technical field of broad-spectrum detection of mycobacteria; the primer-probe combination including forward primer, reverse primer and probe, the nucleotide sequence of forward primer is shown in SEQ ID NOs. 1 and 2, the nucleotide sequence of reverse primer is shown in SEQ ID NO. 3, and the nucleotide sequence of detection probe is shown in SEQ ID NOs. 4-6. The primer-probe combination provided by the present disclose can detect more than 160 types of mycobacteria, covering a wide range; the detection time is greatly shortened; having strong specificity, and has no cross reaction with non-mycobacterial cells and viral genomes; having high detection sensitivity, with the detection limit reaching 100 CFU / ml and the genome detection limit being 10-20 copies / reaction; having high durability and high resistance to matrix interference.

The invention relates to a recombinant Mycobacterium cell for use as a safe and effective vaccine in the prevention of tuberculosis occurrence or recurrence.

The invention belongs to the technical field of bioengineering, and particularly relates to an Mtb8 polyclonal antibody as well as a preparation method and application thereof. The polyclonal antibody is an Mtb8 specific polyclonal antibody obtained by immunizing an animal with cell wall components of mycobacterium tuberculosis, collecting immunized serum and purifying the serum. The polyclonal antibody is only combined with Mtb8 and only reacts with mycobacteria, so that the polyclonal antibody has relatively good specificity and relatively high sensitivity. The polyclonal antibody prepared by the invention can be used for detecting tuberculosis and nontuberculous mycobacterium (NTM) diseases, and is beneficial to improving the accuracy of a detection result.

The present invention includes compositions, including epitope megapools, and methods for detecting the presence of: a Mycobacterium, including a latent or active infection, or an immune response relevant to a Mycobacterium infection including T cells responsive to one or more Mycobacterium peptides or proteins from: one or more amino acid sequences selected from any one of those sequences set forth in Tables 1-5 (SEQ ID NOS: 1-718), or a subsequence, portion, homologue, variant or derivative thereof; a fusion protein; a pool of 2 or more peptides; or a polynucleotide that encodes one or more peptides or proteins, or a subsequence, portion, homologue, variant or derivative thereof. The invention further provides vaccines, diagnostics, therapies, and kits, comprising such proteins or peptides.

The present invention relates to compounds of Formula (I):and pharmaceutically acceptable salts thereof, wherein A, E, and R1 are as defined herein. The present invention also relates to compositions which comprise at least one dihydroisoxazole compound of the invention. The invention also provides methods for inhibiting growth of mycobacterial cells as well as a method of treating mycobacterial infections by Mycobacterium tuberculosis comprising administering a therapeutically effective amount of a dihydroisoxazole of the invention and / or a pharmaceutically acceptable salt thereof, or a composition comprising such compound and / or salt.