Optical tweezers device based on photo-thermal diffusion phoresis and particle control method
A diffusion swimming and photothermal technology, applied in the field of optical tweezers, can solve the problems that the control target is limited to charged nanoparticles, and the thermoelectric optical tweezers device is not universal, and achieve the effect of low optical power density and simple structure
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Embodiment 1
[0083] like figure 2 As shown, the glass substrate 6 at the bottom of the sample cell 5 is provided with a gold layer with a thickness of 10 nm, and polystyrene nanospheres with a diameter of 500 nm, PEG10000 and water are added to the sample cell 5 to form a mixed solution. The mass fraction of the ball is 0.1%, and the mass fraction of PEG10000 is 5%. The laser 71 selects a wavelength of 785 nm, and the laser power at the convergence point of the objective lens 10 is 0.1 mW. After the laser 71 is turned on, the laser beam expands through the beam expander lens group 72, and is incident on the 100-fold oil immersion objective lens 10 through the bandpass reflector 9, And converge to the surface of the gold layer with a thickness of 10nm. The gold layer (photothermal material layer) with a thickness of 10nm generates a heat source with a diameter of about 1μm at the center of the light spot convergence. The temperature gradient field generated around it induces the PEG10000 t...
Embodiment 2
[0085] like figure 2 As shown, a 10nm-thick gold layer is arranged on the glass substrate at the bottom of the sample cell 5, and polystyrene nanospheres with a diameter of 200nm, PEG10000 and water are added to the sample cell 5 to form a mixed solution, and the polystyrene nanospheres in the mixed solution are The mass fraction of PEG10000 is 0.1%, and the mass fraction of PEG10000 is 5%. The laser 71 selects a wavelength of 785 nm, and the laser power at the convergence point of the objective lens 10 is 0.06 mW. After the laser is turned on, the laser beam is expanded through the beam expander lens group 72, and is incident on the 100-fold oil immersion objective lens 10 through the bandpass mirror 9, and The 10nm-thick gold layer (photothermal material layer) generates a heat source with a diameter of about 1 μm at the center of the light spot convergence, and the temperature gradient field generated around it induces the PEG 10000 to generate heat directed to the center ...
Embodiment 3
[0087] like figure 2 As shown, a 10nm-thick gold layer is set on the glass substrate at the bottom of the sample cell 5, and E. coli, PEG10000 and water are added to the sample cell 5 to form a mixed solution, the mass fraction of E. coli in the mixed solution is 0.02%, and the mass of PEG10000 The score is 5%. The laser 71 selects a wavelength of 785 nm, and the laser power at the convergence point of the objective lens 10 is 0.2 mW. After the laser is turned on, the laser beam is expanded through the beam expander lens group 72, and is incident on the 100-fold oil immersion objective lens 10 through the bandpass mirror 9, and The 10nm-thick gold layer (photothermal material layer) generates a heat source with a diameter of about 1 μm at the center of the light spot convergence, and the temperature gradient field generated around it induces the PEG 10000 to generate heat directed to the center of the heat source. Diffusion electrophoresis force 4 acts on Escherichia coli in...
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