Collagen aggregate scaffold material and preparation method thereof

A scaffold material and aggregate technology, applied in prosthesis, medical science and other directions, can solve the problems of no suitable pore structure, flexibility, low mechanical strength of pure collagen scaffolds, etc. site, the effect of promoting tissue repair or regeneration

Active Publication Date: 2022-08-05
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a high-strength, flexible, porous collagen aggregate scaffold material for promoting tissue regeneration and its prepara

Method used

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  • Collagen aggregate scaffold material and preparation method thereof
  • Collagen aggregate scaffold material and preparation method thereof
  • Collagen aggregate scaffold material and preparation method thereof

Examples

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Effect test

Embodiment 1

[0046] This embodiment provides a preferred method for preparing the aggregate scaffold material:

[0047] Collagen aggregates extracted from bovine Achilles tendon, specifically collagen fibrils, were soaked in purified water with a pH of 6 to 7 and a mass fraction concentration of 4%, and stored in a refrigerator at 2 to 8 °C for 4 hours. .

[0048] Next, the fully soaked 4% collagen aggregate solution was transferred to a vacuum homogenizer, the rotation speed was 20,000 rpm, 3 gears, 10S / time, a total of 3 times. Evacuate to remove air bubbles to obtain a uniformly dispersed collagen fibril dispersion.

[0049] Subsequently, the collagen fibril dispersion was slowly poured into a sieve with a diameter of 20 cm. The sieve was covered with an 80-mesh nylon mesh. The solution was slowly shaken, and an 80-mesh nylon mesh was placed on the mesh. Let it air dry for 48 hours at room temperature. Next, the air-dried transparent film was immersed in purified water for 1 h. Pre-...

Embodiment 2

[0054] This embodiment provides a preferred method for preparing the aggregate scaffold material:

[0055] Collagen aggregates extracted from bovine Achilles tendon, specifically collagen fibers, were soaked in PBS reagent, the pH value of PBS reagent was 7.2-7.4, the mass fraction concentration was 2%, and stored in a refrigerator at 2-8 °C for 12 Hour.

[0056] Next, the fully soaked 2% collagen aggregate solution was transferred to a vacuum homogenizer, the rotation speed was 40000 rpm, 5 gears, 30S / time, a total of 1 time. Evacuate to remove air bubbles to obtain a uniformly dispersed collagen fiber dispersion.

[0057] Subsequently, the collagen fiber dispersion was slowly poured into a sieve with a diameter of 10 cm, and the sieve was covered with an 80-mesh nylon mesh, the solution was slowly shaken, and an 80-mesh nylon mesh was placed on it. Let it air dry for 24 hours at room temperature. Next, the air-dried transparent film was immersed in purified water for 3 ho...

Embodiment 3

[0061] Collagen aggregates extracted from bovine Achilles tendon, specifically collagen fiber bundles, were soaked in purified water with a pH of 6 to 7 and a mass fraction concentration of 5%, and stored in a refrigerator at 2 to 8 °C for 24 hours. .

[0062] Next, the fully soaked 5% collagen aggregate solution was transferred to a vacuum homogenizer, the rotation speed was 40000 rpm, 6 gears, 20S / time, a total of 4 times. Evacuate to remove air bubbles to obtain a uniformly dispersed collagen fiber bundle dispersion.

[0063] Subsequently, the collagen fiber bundle dispersion was slowly poured into a sieve with a diameter of 10 cm, and the sieve was covered with an 80-mesh nylon mesh. The solution was slowly shaken, and an 80-mesh nylon mesh was placed on it. Let it air dry for 24 hours at room temperature. Next, the air-dried transparent film was immersed in purified water for 1 h. Pre-freeze at -80°C, quickly transfer it to a lyophilizer, and lyophilize for 48 hours. ...

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Abstract

The invention provides a collagen aggregate scaffold material and a preparation method thereof, and relates to the technical field of biomedical materials. The method comprises the following steps: (A) soaking collagen aggregates extracted from bovine achilles tendons in a medium; (B) transferring the fully soaked collagen aggregate and medium into a vacuum homogenizer to prepare a collagen aggregate dispersion liquid; (C) transferring the collagen aggregate dispersion liquid into a screen or a receiving container, uniformly shaking, and naturally air-drying; (D) immersing the naturally air-dried transparent material into purified water to absorb water and swell, then pre-freezing at a certain freezing temperature and then freeze-drying to obtain a material with a certain interlayer spacing or pore structure; (E) preparing a stent material with certain tensile strength and flexibility by adopting a cross-linking agent under a certain cross-linking condition; and (F) fully cleaning the scaffold material, and then pre-freezing and freeze-drying at a certain freezing temperature to obtain the collagen aggregate scaffold material.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a collagen aggregate scaffold material and a preparation method thereof. Background technique [0002] Collagen is the most abundant and widely distributed structural protein in animals. Collagen makes the extracellular matrix have good mechanical properties and structural integrity, has the function of supporting organs and protecting the body, and plays a key role in maintaining the structure and physiological functions of the body. As a natural biomaterial, collagen has the advantages of good biocompatibility, weak antigenicity and biodegradability. However, pure collagen has poor mechanical strength and flexibility, and it is precisely its lack of mechanical strength and flexibility that limits the wide application of collagen in tissue engineering and regenerative medicine. [0003] In order to overcome the poor mechanical properties of pure collagen, the foll...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/56C08J9/28C08J9/36C08J3/24C08L89/00
CPCC08J9/28C08J9/36C08J3/24A61L27/56A61L27/24C08J2389/00C08J2201/0484C08J2207/10
Inventor 但卫华何晓棠朱美峰但年华
Owner SICHUAN UNIV
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