Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Collagen aggregate scaffold material and preparation method thereof

A scaffold material and aggregate technology, applied in prosthesis, medical science and other directions, can solve the problems of no suitable pore structure, flexibility, low mechanical strength of pure collagen scaffolds, etc. site, the effect of promoting tissue repair or regeneration

Active Publication Date: 2022-08-05
SICHUAN UNIV
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a high-strength, flexible, porous collagen aggregate scaffold material for promoting tissue regeneration and its preparation method, to solve the problems in the prior art that the pure collagen scaffold has low mechanical strength, no suitable pore structure and flexibility. sexual technical issues

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Collagen aggregate scaffold material and preparation method thereof
  • Collagen aggregate scaffold material and preparation method thereof
  • Collagen aggregate scaffold material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] This embodiment provides a preferred method for preparing the aggregate scaffold material:

[0047] Collagen aggregates extracted from bovine Achilles tendon, specifically collagen fibrils, were soaked in purified water with a pH of 6 to 7 and a mass fraction concentration of 4%, and stored in a refrigerator at 2 to 8 °C for 4 hours. .

[0048] Next, the fully soaked 4% collagen aggregate solution was transferred to a vacuum homogenizer, the rotation speed was 20,000 rpm, 3 gears, 10S / time, a total of 3 times. Evacuate to remove air bubbles to obtain a uniformly dispersed collagen fibril dispersion.

[0049] Subsequently, the collagen fibril dispersion was slowly poured into a sieve with a diameter of 20 cm. The sieve was covered with an 80-mesh nylon mesh. The solution was slowly shaken, and an 80-mesh nylon mesh was placed on the mesh. Let it air dry for 48 hours at room temperature. Next, the air-dried transparent film was immersed in purified water for 1 h. Pre-...

Embodiment 2

[0054] This embodiment provides a preferred method for preparing the aggregate scaffold material:

[0055] Collagen aggregates extracted from bovine Achilles tendon, specifically collagen fibers, were soaked in PBS reagent, the pH value of PBS reagent was 7.2-7.4, the mass fraction concentration was 2%, and stored in a refrigerator at 2-8 °C for 12 Hour.

[0056] Next, the fully soaked 2% collagen aggregate solution was transferred to a vacuum homogenizer, the rotation speed was 40000 rpm, 5 gears, 30S / time, a total of 1 time. Evacuate to remove air bubbles to obtain a uniformly dispersed collagen fiber dispersion.

[0057] Subsequently, the collagen fiber dispersion was slowly poured into a sieve with a diameter of 10 cm, and the sieve was covered with an 80-mesh nylon mesh, the solution was slowly shaken, and an 80-mesh nylon mesh was placed on it. Let it air dry for 24 hours at room temperature. Next, the air-dried transparent film was immersed in purified water for 3 ho...

Embodiment 3

[0061] Collagen aggregates extracted from bovine Achilles tendon, specifically collagen fiber bundles, were soaked in purified water with a pH of 6 to 7 and a mass fraction concentration of 5%, and stored in a refrigerator at 2 to 8 °C for 24 hours. .

[0062] Next, the fully soaked 5% collagen aggregate solution was transferred to a vacuum homogenizer, the rotation speed was 40000 rpm, 6 gears, 20S / time, a total of 4 times. Evacuate to remove air bubbles to obtain a uniformly dispersed collagen fiber bundle dispersion.

[0063] Subsequently, the collagen fiber bundle dispersion was slowly poured into a sieve with a diameter of 10 cm, and the sieve was covered with an 80-mesh nylon mesh. The solution was slowly shaken, and an 80-mesh nylon mesh was placed on it. Let it air dry for 24 hours at room temperature. Next, the air-dried transparent film was immersed in purified water for 1 h. Pre-freeze at -80°C, quickly transfer it to a lyophilizer, and lyophilize for 48 hours. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Maximum tensile strengthaaaaaaaaaa
Tensile strengthaaaaaaaaaa
Login to View More

Abstract

The invention provides a collagen aggregate scaffold material and a preparation method thereof, and relates to the technical field of biomedical materials. The method comprises the following steps: (A) soaking collagen aggregates extracted from bovine achilles tendons in a medium; (B) transferring the fully soaked collagen aggregate and medium into a vacuum homogenizer to prepare a collagen aggregate dispersion liquid; (C) transferring the collagen aggregate dispersion liquid into a screen or a receiving container, uniformly shaking, and naturally air-drying; (D) immersing the naturally air-dried transparent material into purified water to absorb water and swell, then pre-freezing at a certain freezing temperature and then freeze-drying to obtain a material with a certain interlayer spacing or pore structure; (E) preparing a stent material with certain tensile strength and flexibility by adopting a cross-linking agent under a certain cross-linking condition; and (F) fully cleaning the scaffold material, and then pre-freezing and freeze-drying at a certain freezing temperature to obtain the collagen aggregate scaffold material.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a collagen aggregate scaffold material and a preparation method thereof. Background technique [0002] Collagen is the most abundant and widely distributed structural protein in animals. Collagen makes the extracellular matrix have good mechanical properties and structural integrity, has the function of supporting organs and protecting the body, and plays a key role in maintaining the structure and physiological functions of the body. As a natural biomaterial, collagen has the advantages of good biocompatibility, weak antigenicity and biodegradability. However, pure collagen has poor mechanical strength and flexibility, and it is precisely its lack of mechanical strength and flexibility that limits the wide application of collagen in tissue engineering and regenerative medicine. [0003] In order to overcome the poor mechanical properties of pure collagen, the foll...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/24A61L27/56C08J9/28C08J9/36C08J3/24C08L89/00
CPCC08J9/28C08J9/36C08J3/24A61L27/56A61L27/24C08J2389/00C08J2201/0484C08J2207/10
Inventor 但卫华何晓棠朱美峰但年华
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com