Osteoinductive material based on fish skin collagen aggregates and its preparation method and use

Abstract

The invention belongs to the field of biological materials and particularly relates to a fishskin collagen aggregates based bone conductive material and a preparation method and application thereof. The material can be widely applied to the field of bone tissue repair and plastic surgery, and the bone conductive material is obtained by taking natural fishskin collagen aggregates with moderate treatment as carriers and plant polyphenols and aldehyde groups as media and solidifying fish bones subjected to pelletizing processing on the surface and internal pores of the fishskin collagen aggregates. The material can be directly prepared by taking the fishskin collagen aggregates as the carriers, better natural bionic three-dimensional structure and better mechanical properties are achieved, and potential risk of human and zoonotic diseases such as mad cow disease and foot and mouth diseased caused by the use of animal source materials such as pigs and cows is avoided.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to an osteoinductive material based on fish skin collagen aggregates, a preparation method and application thereof, and the material can be widely used in the fields of bone tissue repair and plastic surgery. Background technique [0002] There are about 3 million bone defect patients in my country every year due to tumor diseases, accidental disabilities and bone tissue diseases. Bone graft materials are needed to repair the defective bone tissue. The most commonly used in clinical practice is autologous cancellous bone graft. The treatment method of "cutting out flesh and repairing sores" often brings additional pain to patients. Although allogeneic bone materials can overcome the above-mentioned defects, they have inherent defects of limited materials, and it is difficult to benefit the general public. Traditional bone repair materials, including heterogeneous bone,...

AI Technical Summary

Problems solved by technology

However, there are two unavoidable problems in the biomimetic preparation of bone repair materials using this method from the perspective of composition and structure: (1) the collagen used in the prior art is obtained from animal tissues (skin, Achilles tendon, cartilage, squamous etc.), the process is complicated, and more importantly, it is difficult to reproduce the mechanical properties and biological structure of natural collagen fiber tissue even after fiber reorganization or chemical cross-linking modification. In addition, the current Most of the collagen used comes from mammalian tissues, and there are risks of zoonotic diseases such as mad cow disease and foot-and-mouth disease

(2) In order to introduce hydroxyapatite into bone repair materials, it is often mineralized by immersing the organic component collagen in a mineralization solution containing calcium and phosphorus. The mineralization process lasts for a long time, and the operation is cumbersome, which is not conducive to scale chemical production

In response to the above problems, it has also been reported that polylactic acid was used to imitate the network structure of natural collagen aggregates, and polylactic acid fiber bundles were prepared by thermally induced phase separation for inducing bone tissue regeneration (Shao J., Chen C., Wang Y., et al. al.Early stage structural evolution of PLLA porous scaffolds in thermally induced phase separation process and the corresponding biodegradability and biological property[J].Polymer degradation and stability,2012,97:955-963), although its biological structure is closer to natural collagen aggregates , but due to the inherent difference in composition, it still cannot be compared with bone repair materials containing collagen components; at the same time, nano- or micro-hydroxyapatite is directly loaded into collagen hydrogels for the preparation of osteoinductive hydrogels, However, there are still biological structures that cannot be compared with natural collagen fiber weaving; at the same time, the bone tissue engineering that has emerged in recent years, that is, the organic combination of scaffold materials (carriers), seed cells and bioactive factors (growth factors), although in cells and Relatively good results have been achieved in animal experiments, but ultra-low temperature storage is required, which presents many inconveniences in processing, distribution, and use, and there is also a potential risk of infection

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