Fluorescent compound as well as preparation method and application thereof

Abstract

The invention relates to the technical field of fluorescent materials, and discloses a fluorescent compound as well as a preparation method and application thereof. The structural general formula of the fluorescent compound is shown in the specification, R1, R2, R3, R4 and R5 are respectively and independently selected from H and C1-6 alkyl groups, R6 is selected from C1-6 alkyl groups, and X is halogen. The compound has an aggregation-induced emission phenomenon, can emit fluorescence, can perform dyeing and fluorescence labeling on cells and perform accurate positioning on cell nucleuses, and has the advantages of good fluorescence quenching resistance, photobleaching resistance, large Stokes shift and the like; and the compound is non-toxic to various cells in a certain concentration range, and is high in safety.

Description

technical field [0001] The invention relates to the technical field of fluorescent materials, in particular to a fluorescent compound and a preparation method and application thereof. Background technique [0002] Fluorescent probes play a huge role in biological, medical and other fields, and have gradually become a research hotspot. Fluorescent probes can enter cells and identify and locate various subcellular structures in cells, while the nucleus is the largest and most important cellular structure in eukaryotic cells. It is the regulatory center of cell genetics and metabolism. One of the most distinctive signs of a cell. The nucleus plays a key role in a variety of cellular activities such as metabolism, reproduction and genetics, so nuclear stains are an important means to study the morphology and function of the nucleus. [0003] A variety of nuclear stains have been developed, and Hoechst33342, DAPI (4',6-diamidino-2-phenylindole), etc. are currently used as scien...

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Problems solved by technology

[0006] 2. The colocalization staining is not accurate, which means that not only the nucleus is stained, but also the cytoplasm outside the nucleus is also stained during the cell staining process;

[0007] 3. The Stokes shift is too small, so that the overlap between the absorption and emission spectra is too large, resulting in a decrease in fluorescence efficiency due to energy transfer

[0008] 4. It is prone to photobleaching, which is manifested that when the intensity of the excitation light exceeds a certain limit, the light absorption tends to be saturated, and the fluorescence quenching is serious

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