Intracellular and extracellular photoelectric integrated detection platform as well as construction method and application thereof
A detection platform and photoelectric detection technology, applied in the field of spectral imaging and biological sensing, to achieve the effect of low cost, high efficiency and excellent performance
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Embodiment 1
[0063] Example 1 Collection of blank signals of photoelectric integrated detection platform inside and outside cells
[0064] like Image 6 Shown is the fluorescence spectrum of the ratiometric nano-fluorescent probe, which quantitatively analyzes the analyte by fluorescence intensity ratio. Among them, the reference signal peak at 700nm, with constant intensity; Ca at 600nm 2+ Signal peak, intensity varies with Ca 2+ The concentration decreased; the pH signal peak was at 515nm, and the intensity decreased with the decrease of pH. F 600 / 700 with Ca 2+ Concentration is linear between 1-300 μM; F 515 / 700 Linear relationship with pH between 8.0–5.73.
[0065] like Figure 7 Shown are the fluorescence signal and photoelectric signal of the fiber itself under the excitation of 488nm light by the photoelectric integrated detection platform inside and outside the cell. It can be seen that the fiber itself has no autofluorescence at 515nm, 600nm, and 700nm, and the weak photocu...
Embodiment 2
[0066] Example 2 Intracellular and extracellular photoelectric integrated detection platform is used for simultaneous collection of intracellular fluorescence signal and extracellular photocurrent signal in the cell layer
[0067] In order to evaluate the effect of the integrated intracellular and extracellular photoelectric detection platform on the simultaneous collection of intracellular fluorescence signals and extracellular photocurrent signals in the solution cell layer, the ratiometric nano-fluorescent probe (1mM) was incubated with cells for 30 minutes and used for signal collection. .
[0068] like Figure 8 Shown is a schematic diagram of the fiber targeting the intercellular space. The diameter of the fiber electrode tip is about 500nm, which can be accurately inserted into the neuron synaptic space and measure.
[0069] like Figure 9 It shows the intracellular fluorescence signal and extracellular photocurrent signal in the cell layer. After positioning the fibe...
Embodiment 3
[0070] Example 3 Intracellular and extracellular photoelectric integrated detection platform is used for simultaneous collection of intracellular fluorescence signal and extracellular photocurrent signal in the living body
[0071] The intracellular and extracellular photoelectric integrated detection platform can be used to simultaneously collect intracellular fluorescence signals and extracellular photocurrent signals in different brain regions. First, the ability of optical fibers to target brain regions is evaluated. like Figure 10 shown, Figure 10 It is a schematic diagram of the fiber targeting different brain regions in this embodiment, including cortex, hippocampus, striatum, and thalamus. The dotted circle is the location of the electrode, and the optical fiber can effectively target brain regions such as the cerebral cortex, hippocampus, striatum, and thalamus. Subsequently, the ratiometric nano-fluorescent probes were incubated with the brain slices for 30 minut...
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