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Diagnostic reagent based on tetrazole compound and diagnostic band thereof

A technology of reagent strips and reagents, applied in the field of diagnostic compositions, capable of solving problems such as destruction

Inactive Publication Date: 2004-06-30
LIFESCAN INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While ferricyanides are known to convert hemoglobin to methemoglobin, they also destroy the intended product NADH

Method used

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  • Diagnostic reagent based on tetrazole compound and diagnostic band thereof
  • Diagnostic reagent based on tetrazole compound and diagnostic band thereof
  • Diagnostic reagent based on tetrazole compound and diagnostic band thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A 0.8 μm nylon film purchased from Cuno (Meriden, CT, USA) was immersed in the reagents in Table 1 until saturated. Gently scrape off excess reagent with a glass rod. The resulting film was hung in an oven at 56°C for 10 minutes to dry. The Porex (0.6mm thick) was soaked in the nitrite solution of Table 2, then hung in a 100°C oven to dry for 10 hours. Finally, in polyester raw material (0.4mmMelenex purchased from American ICI, Wilmington, DE  polyester) and Porex impregnated with nitrite.

[0046] components

[0047] components

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PUM

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Abstract

A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises dehydrogenase enzyme that has specificity for the analyte, NAD, an NAD derivative, pyrrolo-quinoline quinone (PQQ), or a PQQ derivative, a tetrazolium dye precursor, a diaphorase enzyme or an analog thereof, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring ketone bodies, such as beta-hydroxybutyrate.

Description

technical field [0001] The present invention relates to a diagnostic composition for determining the concentration of an analyte in a biological fluid containing hemoglobin. The composition is based on tetrazolium dye precursors and involves the inhibition of their hemoglobin-induced reduction. The invention also relates to test strips based on the diagnostic composition. Background technique [0002] Adipose tissue is one of the most abundant forms of energy storage in the body. It releases stored fatty acids into the circulation, where they are metabolized primarily by the liver. During this process, fatty acids are consumed and energy is released and used by the body. Normally, little fat is consumed, and fatty acids are completely metabolized into carbon dioxide and water, and this conversion does not disturb the body's sensitive pH balance. However, if the amount of carbohydrates in the body is deficient, for example due to diet, fat consumption and fatty acid produ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50C12Q1/32C12Q1/54G01N33/52G01N33/64G01N33/66G01N33/68G01N33/72
CPCC12Q1/32G01N33/64Y10S435/962Y10S435/97G01N33/525Y10T436/173076
Inventor 欧阳天美余绍炀
Owner LIFESCAN INC
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