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Mannosan peptide and its prepn and use

A mannan peptide and composition technology, applied in the field of mannan peptide composition, can solve the problems of large oral dosage and complex ingredients

Inactive Publication Date: 2004-10-27
成都利尔药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese patent 98121898 discloses "a kind of compound mannan peptide oral liquid", which contains multiple components, and its mannan peptides are from basidiomycete fungi, Septobasidiomycetes fungi, Polyporacea fungi, and Umbelliferae fungi. complex, large oral dose

Method used

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  • Mannosan peptide and its prepn and use
  • Mannosan peptide and its prepn and use

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0022] Experimental example 1, preparation of mannan peptide:

[0023] Fermentation process: i, the preparation of bacterial classification: select bacterial classification to be α-hemolytic streptococcus (Streptococcushemolyticus-α-hemolysis);

[0024] Culture medium: strain culture medium: glucose 0.4-0.8%, sodium chloride 0.4-0.8%, protein

[0025] Peptone 0.4-1%, beef extract 0.1-0.5%, sheep blood 10%, agar 1.5-2%,

[0026] PH7.2-7.8

[0027] Fermentation medium: glucose 0.1-0.5%, peptone 0.4-0.7%, yeast extract 0.4-0.6%,

[0028] Sodium chloride 0.4-0.8%, bubble enemy 0.01%

[0029] Raw and auxiliary materials required for extraction and refining processes: alcohol, acetone, trichloroacetic acid, absolute ethanol

[0030] a. Mother bottle preparation: Unseal the strain freezing tube, dilute it with sterile broth medium, and insert it into the blood slant under aseptic conditions. 18-45 ℃ constant temperature culture for 10-30h;

[0031] b...

experiment example 2

[0045] Experimental Example 2: Identification of Mannan Peptides

[0046] 1. Identification of strains: see the identification records of strains.

[0047] 2. Identification of semi-finished products:

[0048] i. Determination of absorbance: take an appropriate amount of semi-finished product sample, add water to make a solution containing 0.4mg in every 1ml, according to spectrophotometry (Chinese Pharmacopoeia 2000 edition two appendix VI A), at the wavelength of 260nm, its absorbance must not be greater than 0.25, at the wavelength of 280nm, its absorbance shall not be greater than 0.20.

[0049] ii. Content determination:

[0050] a. Preparation of reference substance solution Accurately weigh 0.1g of D-mannose reference substance dried at 105°C to constant weight, put it in a 100ml measuring bottle, add water to dissolve and dilute to the mark, shake well; precisely measure 5ml, put it in a 100ml In a measuring bottle, add water to the mark and shake well. Each 1ml cont...

experiment example 3

[0078] Experimental Example 3: Determination of the weight average molecular weight of the mannan peptide prepared in Experimental Example 1

[0079] 1. Instrument

[0080] Shimadzu high performance liquid chromatography (including LC-10AT pump, CTO-10A constant temperature column oven, DGU-14A degassing device, RID-10A differential refractive index detector); TSK-G4000PWXL (or Shodex Ohpak SB-804HQ) chromatographic column ; Longzhida interface, computer (built-in special GPC software for polysaccharides); 50ul syringe; Millipore ultrapure water device; 0.45um filter membrane column head filter, vacuum filtration device; analytical balance.

[0081] 2. Preparation of reagents and samples

[0082] 1. Ultrapure water

[0083] 2. Column storage solution: 0.05 sodium azide solution (NaN3) solution

[0084] 3. Mobile phase: 0.7% sodium sulfate solution (containing 0.02% NaN3)

[0085] 4. The test solution: take appropriate amount of raw materials respectively, add mobile phase ...

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Abstract

The mannosan peptide is a kind of fermentation product from Streptococcus hemolyticus-alpha. The mannosan peptide has weight average molecular weight of 10-200 KD and molecular weight distributing coefficient below 4.0. The present invention also provides the preparation process and use of the mannosan peptide.

Description

technical field [0001] The present invention provides a mannan peptide composition and its preparation process and application, specifically, mannan from a fermentation product of Streptococcus hemolyticus-α-Hemolysis with a certain weight-average molecular weight range Glycan peptide and its preparation process and use. Background technique [0002] As the environment changes, the incidence of human tumors continues to increase, and the age of onset is also lower than in the past. Finding safe and effective anti-tumor drugs is the goal that human beings have been working hard for. [0003] Tumor treatment through the body's defense mechanism was advocated by the National Cancer Institute of the United States in the late 1970s, and gradually developed worldwide in the 1980s. Japan is at the forefront in the research and development of immune activators against tumors, and has been approved for production and marketing. Streptococcus preparations of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61P7/00A61P11/00A61P35/00A61P37/04C07K14/315C12P21/00
Inventor 陈云华余清和李军李丽刘艳
Owner 成都利尔药业有限公司
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