Method of preparing transparent sodium protonate from transparent protonic acid fermentation liquid

Abstract

The invention discloses a method for preparing sodium hyaluronate from hyaluronic acid fermentation broth, which involves preparing sodium hyaluronate with high molecular weight and low protein content from hyaluronic acid fermentation broth. The invention prepares sodium hyaluronate from fermentation liquid by heating to remove bacteria, adding protease to remove protein, and using ultrafiltration technology to remove small metal ion molecules and colored substances. The specific process is that the hyaluronic acid fermentation broth undergoes bacteria removal, enzymolysis, ethanol precipitation, complexation, dissociation, ethanol precipitation, ultrafiltration, ethanol precipitation, washing, dehydration and drying, and finished product packaging to obtain a molecular weight of 1.8×10 6 , Sodium hyaluronate with a protein content of 0.30% and an extraction yield of 98%. The method of the invention has the advantages of simple and convenient operation, high efficiency, low cost, high purity of the finished product, little environmental pollution, easy industrialization, etc., not only can be conveniently implemented in a laboratory, but also very easy to be used in industrial production.

Description

technical field [0001] The invention relates to a method for preparing sodium hyaluronate from hyaluronic acid fermentation liquid, in particular to preparing sodium hyaluronate with high molecular weight and low protein content from hyaluronic acid fermentation liquid. Background technique [0002] Hyaluronic acid (Hyaluronic acid, HA), also known as hyaluronic acid or hyaluronic acid, is a macromolecular mucopolysaccharide widely present in connective tissues of organisms. The physiological functions of HA are different in different tissues, such as water retention in the skin, lubrication in the synovial fluid of the joints, and permeability regulation in the blood vessel wall. In addition, as a polyanion electrolyte, HA has a large number of negative charges on the molecule, which can adjust the concentration of positive and negative ions around it and inhibit the activity of various enzymes. It is widely used in ophthalmic viscous surgery, treatment of joint diseases, s...

AI Technical Summary

Problems solved by technology

However, the disadvantages of this method are: 1) even after repeated extractions, there is still a certain amount of bacteria in the finished product HA, so it is difficult to improve the extraction rate; 2) a large amount of organic solvent is used, although the solvent can be recycled, but still Cause huge consumption of raw materials and increase the extraction cost; 3) The use of a large amount of toxic, explosive and volatile solvents has caused serious pollution to the environment and brought inconvenience to the operation

Although fractional separation with ethanol can effectively remove metal ions, this method is cumbersome and expensive to operate, and it is difficult to adapt to industrial production; colored substances are another type of impurities commonly found in HA, and the commonly used removal methods include oxidation and reduction. However, these two methods will cause oxidation of the terminal aldehyde group of HA, damage the activity of HA, desulfation and degradation reactions

[0007] The previous HA extraction methods generally have disadvantages such as high cost, low extraction rate, poor and unstable quality, and serious environmental pollution, which are difficult to meet the needs of industrial production and practical application.