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Yeast heat resistant related gene and its use

A yeast and gene technology, applied in the fields of biotechnology and industrial microbiology, can solve problems such as increasing production operating costs and reducing the temperature of fermentation broth

Inactive Publication Date: 2006-07-19
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even in spring and autumn, refrigeration equipment should be used to reduce the temperature of the fermentation liquid, which greatly increases the production and operation costs

Method used

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  • Yeast heat resistant related gene and its use
  • Yeast heat resistant related gene and its use
  • Yeast heat resistant related gene and its use

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0051] 1. Experimental method:

[0052] 1. Yeast inoculation and cultivation: medium composition glucose 2%, peptone 2%, yeast extract 1%, strain yeastY-179, culture conditions 30 degrees and 45 degrees under constant temperature for 48 hours.

[0053] 2. Extraction of total RNA

[0054] According to the instruction manual of TRIZOL Total RNA Extraction Reagent, the specific operation is as follows:

[0055] 1) Take 0.1 gram of fresh tissue, cut it into pieces with scissors, put it into a glass homogenizer containing 1ml TRIZOL solution and homogenize thoroughly.

[0056] 2) Aspirate the tissue homogenate into a 50ml centrifuge tube and let stand at room temperature for 5 minutes.

[0057] 3) Add 0.2ml of chloroform, shake vigorously for 15 seconds and mix well, then let stand at room temperature for 3 minutes.

[0058] 4) Centrifuge at 10,000g at 4°C for 15 minutes, and the RNA is distributed in the water phase.

[0059] 5) Transfer the upper colorless aqueous phase to an...

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Abstract

A kind of genes associated with the thermotolerance of yeast, especially two novel ESTs associated with the thermotolerance of Kluyveromyces marxianus is disclosed. A process for finding out them is also disclosed.

Description

technical field [0001] The present invention belongs to biotechnology and industrial microbiology. The invention relates to yeast thermostable genes and their application, as well as industrial strains and crop varieties improved by using these thermostable genes. Background technique [0002] With the completion of the sequencing of the Saccharomyces cerevisiae genome in 1996, the research attention of scientific workers in related fields quickly shifted from gene structure to gene function, and the "battle for genes" began rapidly. Thermosaccharomyces is a thermostable eukaryotic organism. Studying the overall expression of its thermotolerance-related genes from the level of genomics (rather than the behavior of a single gene) can not only obtain the autonomy of such genes, but also gain an in-depth understanding of the thermostable mechanism of thermosaccharomyces , and more importantly, the understanding of the nature of yeast stress resistance gene regulation can provi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C07H21/04C12P19/34C12N15/63C12N15/31
Inventor 文铁桥鲍奎一曹子谦陈付学宋红生李育阳
Owner SHANGHAI UNIV