Method for enzyme hydrolyzing zein through two steps method
A zein and method enzyme technology, which is applied in the technical field of zein hydrolysis, can solve the problems of ineffective production of corn depressor peptide, insoluble prolamin components, prolaminase inability to hydrolyze prolamin, etc. Achieve high social and economic benefits, increase added value, and high hydrolysis efficiency
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Embodiment 1
[0028] 1 Weigh 3 grams of zein and fully dissolve it with 150mL 65% ethanol solution to make a 2% protein ethanol solution, and adjust the pH value to 8.0 with 0.2mol / L NaOH solution;
[0029] 2 Prepare an alkaline protease with disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with a pH value of 8.0 to prepare 1% enzyme solution (enzyme activity unit is 1800U / mL);
[0030] 3 The first enzymatic hydrolysis: Measure 150mL of protein solution, add 6mL of 1% protease solution, mix well, enzymolyze in a water bath at 48°C, add 0.1mol / L NaOH solution dropwise, and keep the pH value of the solution at 8.0;
[0031] 4 After 20 minutes of enzymatic hydrolysis, the reaction solution was quickly distilled under reduced pressure at 50°C, and stopped after distilling out 35mL of ethanol solution;
[0032] 5 Add deionized water to the above concentrated solution to 150mL, adjust the pH to 8.0 with 0.1mol / L NaOH solution, then add 14mL 1% protease solution for hydrolys...
Embodiment 2
[0035] 1 Weigh 7.5 grams of zein and fully dissolve it with 150mL 75% ethanol solution to make a 5% protein ethanol solution, and adjust the pH value to 8.0 with 0.25mol / L KOH solution;
[0036] 2 Prepare an alkaline protease with disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with a pH value of 8.0 to prepare 1% enzyme solution (enzyme activity unit is 1800U / mL);
[0037] 3 The first enzymatic hydrolysis: Measure 150mL of protein solution, add 25mL of 1% protease solution, mix well, enzymolyze in a water bath at 55°C, add 0.1mol / L NaOH solution dropwise, and keep the pH value of the solution at 8.0;
[0038] 4 After 20 minutes of enzymatic hydrolysis reaction, the reaction solution was quickly distilled under reduced pressure at 50°C, and stopped after distilling out 65mL of ethanol solution;
[0039] 5 Add deionized water to the above concentrated solution to 150mL, adjust the pH to 8.0 with 0.25mol / L KOH solution, then add 25mL of 1% protease soluti...
Embodiment 3
[0042] 1 Weigh 4.5 grams of zein and fully dissolve it with 150mL 70% isoalcohol solution to make a 3% protein ethanol solution, and adjust the pH value to 8.0 with 0.5mol / L NaOH solution;
[0043] 2 Prepare an alkaline protease with disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with a pH value of 8.0 to prepare 1% enzyme solution (enzyme activity unit is 1800U / mL);
[0044] 3 The first enzymatic hydrolysis: Measure 150mL of protein solution, add 18mL of 1% protease solution, mix well, enzymolyze in a water bath at 60°C, add 0.1mol / L NaOH or KOH solution dropwise to keep the pH of the solution at 8.0;
[0045] 4 After 20 minutes of enzymatic hydrolysis, the reaction solution was quickly distilled under reduced pressure at 50°C, and stopped after distilling out 55mL of ethanol solution;
[0046] 5 Add deionized water to the above concentrated solution to 150mL, adjust the pH to 8.0 with 0.5mol / L NaOH solution, then add 18mL of 1% protease solution for...
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