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Anti-yellow diarrhea anti-white diarrhea pathogen egg yolk antibody for piglets and its preparation and use

A technology for piglet yellow dysentery and yolk antibodies, applied in the fields of application, antibodies, antibacterial drugs, etc., can solve problems such as ineffectiveness of immunoglobulins, adverse reactions of antibiotics and drugs, increase the slaughter rate of pigs, etc., and achieve an ideal extraction recovery rate , considerable economic benefits, the effect of maintaining activity and potency

Inactive Publication Date: 2003-08-27
重庆和润实业(集团)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It solves the problem of high impurity content of biological preparations, poultry and veterinary drugs, ineffectiveness of immunoglobulin, many restricted conditions, and its homology and efficacy are higher than other biological preparations; at the same time, it solves the problem of adverse drug reactions of antibiotics, thus Improve the quality of reserve pigs, increase the slaughter rate of pigs, and reduce the cost of medicine and pig raising

Method used

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  • Anti-yellow diarrhea anti-white diarrhea pathogen egg yolk antibody for piglets and its preparation and use
  • Anti-yellow diarrhea anti-white diarrhea pathogen egg yolk antibody for piglets and its preparation and use

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1: the product of the compound yolk antibody of yellow scour of piglets and pullorum pathogenic bacteria (Escherichia coli) with antibody activity can be obtained through the following steps: prepare yellow scours of piglets and pullorum pathogenic bacteria (Escherichia coli) antigens, and immune healthy hens with antigens, Then the eggs laid by them were collected, and anti-EitIgY was extracted from the eggs laid by them.

Embodiment 2

[0035] Example 2: Preparation of bacterial antigens, pathogenic bacteria strains were expanded and cultured in nutrient broth medium for 24 hours. Collect the bacterial cells, add complete Freund's adjuvant at a weight ratio of 1:1, and emulsify to obtain bacterial cell antigens.

Embodiment 3

[0036] Embodiment 3: preparation of pilus subunit antigen, bacterial classification is inoculated in nutrient broth in the ratio of 1: 100 and cultivated for 72h at 37°C, then centrifuged at 3000g at 4°C for 10min, and the precipitated thalline is washed with Tris-HCl buffer (0.05mol / L), high-speed stirring at 60°C for 30min, centrifugation at 11000g at 4°C for 20min, taking the supernatant and repeating centrifugation once, the final supernatant is the crude pili extract. Add 1mL magnesium chloride solution to the crude extract of pili to make the concentration 0.1mol / L, let it stand at 4°C for more than 12h, centrifuge at 24000g for 45min at 4°C, and collect the pili precipitate. Add complete Freund's adjuvant at a weight ratio of 1:1, and emulsify to obtain pili subunit antigen.

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PUM

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Abstract

The invention refers to an anti-piglet yellow and white diarrhoea pathogenicbacteria yolk antibody (anti-EitIgY), the making method and its application of making drugs curing relative diseases caused by infection of piglet pathogenicbacteria (coliform) or feed additive. Test anti-EitIgY according to SDS-PAGE electrophoresis (7.5% separating glue). Its chart showing single band; colorize blue by R250; use immune mark Western-blotting to test the clear peculiar enzyme-dyed stripes appearing in medium-high molecular weight band; adopts the biochemical method of double-contracted urea to determine protein concentration. The method: making the piglet yellow and white diarrhoea pathogenicbacteria antigen, make immunity on healthy hens by the antigen, collecting eggs, and then extracting anti-EitIgY from the eggs.

Description

technical field [0001] The invention relates to an egg yolk antibody substance with antibody activity—anti-yolk antibody (anti-EitIgY) product against piglet yellow scour and pullorum pathogenic bacteria, and relates to its preparation process and application in veterinary pharmacy and feed additive. technical background [0002] Yellow dysentery of piglets is an acute and highly lethal diarrheal infectious disease caused by a certain serotype of hemolytic (pathogenic) Escherichia coli. Infectious disease, the diseased pigs are characterized by the discharge of yellow or yellow-white watery feces and rapid death, and the morbidity and mortality are high, mainly occurring in newborn piglets within 5 days of age, and the incidence of piglets within 3 days of age can reach More than 90%, the incidence of a litter of newborn piglets is often more than 90%, the mortality rate is about 50%, and sometimes the whole litter dies. Piglet pullorum is a non-septic, acute intestinal inf...

Claims

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Application Information

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IPC IPC(8): A23K20/195A61K39/395A61P31/04C07K16/02
Inventor 雁杰陈君
Owner 重庆和润实业(集团)有限公司