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Chi lucid ganoderma polysaccharide and prep. and use thereof

A Ganoderma lucidum polysaccharide and polysaccharide technology, applied in the field of anti-tumor drugs, can solve the problems of low molecular weight Ganoderma lucidum polysaccharide anti-tumor research, and achieve the effects of good water solubility, low viscosity, and convenient administration

Inactive Publication Date: 2003-10-15
南京美新诺医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the molecular weights of Ganoderma lucidum polysaccharides reported to have anti-tumor effects are high-molecular-weight (tens of thousands to hundreds of thousands) polysaccharides, and there are few anti-tumor studies on low-molecular weight Ganoderma lucidum polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Red Ganoderma lucidum polysaccharide crude product GLP L preparation of

[0029] Weigh 50 grams of red ganoderma lucidum fruiting body powder, add 1000 mL of distilled water, extract in boiling water bath for 10 hours, and stir intermittently. Centrifuge at 3000rpm to remove the precipitate to obtain the extract. Add 500 mL of distilled water to the residue after centrifugation, extract in a boiling water bath for 6 hours, and remove the residue after centrifugation. Combine the supernatants from the two extractions. The supernatant was concentrated to 500 mL. Add 1 / 5 volume (100mL) of chloroform and 1 / 5 volume (20mL) of n-butanol of the concentrated solution, shake vigorously, let stand and separate layers until there is no precipitation at the interface between chloroform and water, remove the lower organic solvent layer and In the middle layer, retain the water layer to remove protein, repeat 5 times, dialyze the obtained solution against flowing water...

Embodiment 2

[0032] Example 2. Red Ganoderma lucidum polysaccharide crude product GLP L The pilot extraction process

[0033] Weigh 20kg of red ganoderma fruiting body powder, add 400L of deionized water, put it into an extraction tank, and extract under stirring at 100°C for 10h. Add 200L deionized water to the residue after centrifugation, extract at 100°C for 6 hours, and remove the residue after centrifugation. Combine the supernatants from the two extractions. The supernatant was concentrated to 200 L at 100°C. Add 1 / 5 volume of chloroform (40 L) and 1 / 5 chloroform volume of n-butanol (8 L) to the concentrated solution, stir for 30 min, deflate, let stand to separate layers, and siphon to obtain supernatant. The deproteinization operation was repeated 3 times. The deproteinized liquid (volume v 0 ) back to the vacuum tank for vacuuming (60°C, 30min), slowly add 3 / 4 times v 0 volume of industrial ethanol, placed at 4°C for 4h, centrifuged, and retained supernatant (volume v 1 ),...

Embodiment 3

[0034] Example 3. Single polysaccharide GLP L1 and GLP L2 preparation of

[0035] 100mg red ganoderma polysaccharide crude GLP L Fully dissolved in 5mL of distilled water, loaded on a well-balanced boric acid type DE-32 ion exchange column (1.9×20cm), the sample loading flow rate was 0.27mL / min, eluted with 146mL of distilled water and 56mL of 0.05mol / L borax solution respectively, the flow rate 20mL / h, collect the eluate step by step, track and detect the sugar content by anthrone sulfate colorimetry, combine the same components, dialyze the eluate from the first and second parts of the effluent, and freeze-dry to obtain the red ganoderma lucidum of the present invention Single polysaccharide finished product: GLP L1 and GLP L2 .

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PUM

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Abstract

The present invention obtains one kind of coarse red glossy ganoderma polysaccharide product and two kinds of single red glossy ganoderma polysaccharide products. Red glossy ganoderma sporophore powder is processed through the steps of water extraction, concentration, centrifugating after adding ethanol to obtain supernatant liquid, adding ethanol and centrifugating to obtain the precipitate as the coarse red glossy ganoderma polysaccharide product with molecular weight 4000-6000. The coarse product is dissolved in water and eluted in boric acid type ion exchange column with distilled waterand 0.05 mol / L boric acid solution successively and the first effluent and the second effluent are collected separately, dialyzed and freeze dried to obtain single red glossy ganoderma polysaccharideproducts GLPL1 and GLPL2 of average molecular weight 4100 and 5700 separately. All the products has tumor growth inhibiting effect and may be used in preparing antitumor medicine.

Description

1. Technical field [0001] The invention relates to red ganoderma polysaccharide extracted from red ganoderma lucidum (Leys.Fr.) Karst] fruit body powder and a preparation method thereof, and also relates to antitumor drugs. 2. Background technology [0002] Because tumor cells are highly similar to certain normal cells in the human body, most chemotherapy drugs that can directly kill cancer cells can also kill those normal cells, which is why most chemotherapy drugs produce toxic and side effects. Because the occurrence and development of tumors are closely related to the immune ability of the body, some experts put forward the concept of "tumor immunotherapy": there are many immune factors in the normal human body, which can effectively attack cancer cells, for example, lentinan , Yunzhi polysaccharide has a wide range of immune regulation and anti-tumor activities, and has been approved as a commonly used anti-cancer drug in clinical practice. This kind of therapy by impr...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P35/00C08B37/00
Inventor 姚文兵高向东赵世华
Owner 南京美新诺医药科技有限公司
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