Method of leaching pairs polyphylla saponin

A saponin and leaching technology of the saponin, which is applied in the field of extracting the active ingredients of the plant, can solve the problems of large amount of alcohol and long leaching time, and achieve the effects of increasing the leaching rate, shortening the leaching time, reducing the loss of alcohol and the loss of active ingredients

Inactive Publication Date: 2004-04-21
YUNNAN BAIYAO GROUP
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The leaching time is long and the amount of alcohol is large, which i

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1.1 Chromatographic conditions HP1100 chromatographic instrument, chromatographic column: Symmetry C8 analytical column (4.6mm×250mm), mobile phase: acetonitrile: water = 45:55, detection wavelength: 203 μm, column temperature: 40°C.

[0016] 1.2 Preparation of the test solution. Dry and pulverize the eucalyptus. Accurately weigh 10 grams of eucalyptus, soak it in 10 times the amount (V / W) of warm water (40°C) for 2 to 3 times, each time for no less than 2 hours, press the Orthogonal table arrangement test, reflux leaching in 10 times the amount of solvent, filter while it is hot, combine the filtrate, extract the filtrate with saturated n-butanol with water, collect the n-butanol layer liquid, evaporate to dryness, dissolve in methanol and dilute it in a 10ml bottle , HPLC method to measure its saponin content.

[0017] 1.3 Preparation of Standard Substance Reference Substance Solution (1) Accurately weigh the standard samples I, II, III, and IV of pachyrhiza saponin d...

Embodiment 2

[0019] Take three samples of 10g of Chonglou, immerse in 10 times the amount (W / V) of warm water (40°C) for 2 to 3 times, and filter for 2 hours each time. Finally, water, 10% alcohol, and 20% alcohol were boiled to carry out the leaching test respectively, and the leaching was carried out three times, each time not less than 2 hours. Results: The first leaching solutions of the three leaching agents were all khaki or brown viscous liquids with poor fluidity and could not be filtered with filter paper, cloth bag, three-layer gauze, cotton layer, etc. The situation of the second and third filtrates was different. better. After the α-amylase substance is added, the above-mentioned first leaching solution can be filtered by any of the above-mentioned filtration methods.

[0020] The results of the orthogonal test I investigated the influence of three factors such as the amount of α-amylase, leaching temperature, and leaching times on the leaching rate of total glucosides of S. p...

Embodiment 3

[0022] Orthogonal Test II and Results According to the results of Test I, the α-amylase was added each time, and the influence of three factors, such as leaching temperature, leaching times, and alcohol concentration, on the leaching rate of total glucosides of S. polyphylla was investigated. The results are shown in Tables 3 and 4.

[0023] Table 1 Factor level table

[0024] Level I (temperature) II α-amylase III (number of leaching)

[0025] 1 60℃ 1 part 2

[0026] 2 80℃ 2 parts 3

[0027] 3 boil 3 servings 4

[0028] Table 2 Orthogonal experiment design table and results

[0029] No. I II III Results (%)

[0030] 1 1 1 1 49.76

[0031] 2 1 2 2 47.2 7

[0032] 3 1 3 3 61.17

[0033] 4 2 1 2 51.90

[0034] 5 2 2 3 65.49

[0035] 6 2 3 1 40.60

[0036] 7 3 1 3 70.82

[0037] 8 3 2 2 54.00

[0038] 9 3 3 1 63.76

[0039] 1 part of α-amylase, boiled, leached 4 times, the leaching rate is 70.82%,

[0040] optimal condition

[0041] ...

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Abstract

A immersing process for extracting the paris polyphylla's saponin includes such steps as pulverizing the pairs polyphylla to obtain coarse powder, immersing in warm water at 40 deg.C for 2-3 hr, filtering, adding alcohol and alpha-amylase, boiling and reflux extracting three times (2 hr for each). Its advantages are short period, and high extracting percentage (more than 97%).

Description

technical field [0001] The present invention relates to a method for extracting the effective components of Paprika pachyrhiza, more specifically, the invention relates to a water leaching method for papaya papaya saponin. Background technique [0002] Pairs Polyphylla Smith Var. Yunnanensis [Franch.] Hand-Mazz or Paris Polyphylla Smith Var. Chinensis [Franch] Hara are rhizomes of Liliaceae plants. This taste is bitter, slightly cold, and slightly poisonous; it can clear away heat and detoxify, reduce swelling and relieve pain, cool the liver and relieve convulsions. For the treatment of sores and carbuncles, sore throat, snake bites, traumatic injuries, convulsions and convulsions. Pharmacological experiments have antibacterial, analgesic and antiasthmatic effects; decoctions and saponins have hemolytic effects (see "The Pharmacopoeia of the People's Republic of China" 2000 edition one). The research on the chemical composition of Chong Building s...

Claims

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Application Information

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IPC IPC(8): C07G3/00C12P19/44C12P33/00
Inventor 王真万近福高崇昆董明
Owner YUNNAN BAIYAO GROUP
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