Artificial bone material
A kind of bone and artificial technology, applied in the direction of bone implants, ceramic products, prostheses, etc., can solve the problems of great influence of raw materials, poor manufacturing process, etc., to achieve the effect of ensuring bone formation, promoting bone formation, and improving practicability
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Embodiment 1
[0036] Calcium carbonate powder and calcium hydrogen phosphate dihydrate in a molar ratio of 1:2 were weighed, put into a ball mill bowl together with pure water, and mixed and pulverized by a ball mill for about 1 day. The resulting slurry mixture was dried at about 80°C and then calcined at 750°C. The obtained powder is β-TCP ceramic with excellent sinterability and high purity.
[0037] Add pure water, ammonium acrylate deflocculant and polyoxyethylene alkylphenyl ether surfactant to the powder, mix and stir to prepare foaming mud. The foamed slurry was dried and then fired at 150°C to obtain β-TCP porous ceramics. The porosity of the porous ceramics is 75%, and the pore diameters are distributed in two ranges of 100-500 μm and 1-0.1 μm.
Embodiment 2
[0039] Using the β-TCP porous ceramic made in Example 1 as the basic material, seed the first-generation cultured cells of osteoblasts derived from bone marrow, culture them in vitro, form the bone tissue of the bone formation seed, and implant them into the organism. From the implanted Tissues form large amounts of bone tissue. The specific method is as follows.
[0040] Take the bone marrow cells, inoculate them into culture flasks, add 10-15% FBS (fetal bovine serum) to the MEM medium, and add 5% CO 2 , cultured at 37°C for about 10 days. Cells were then detached from the culture flasks by trypsinization and subsequently seeded into bulk porous ceramics formed from β-TCP. As a medium, MEM medium supplemented with 10 to 15% of FBS (fetal bovine serum) was used.
[0041]When seeding a 5 mm × 5 mm × 5 mm bulk porous ceramic, a medium with a cell concentration of 1 cc contains 1 million or more cells. at 5% CO 2 After culturing at 37°C for 1-3 hours, replace the medium, an...
Embodiment 3
[0044] The β-TCP porous ceramics produced in Example 1 were seeded by adsorbing cell proliferation factors. As cell growth factors, VEGF, BMP, FGF, FGF-β, IGF, and PDGF were used, respectively. Cultured in vitro, bone tissue that forms bone-forming seeds, implanted into a living body, and forms a large amount of bone tissue from the implanted tissue. The specific method is as follows.
[0045] Take the above-mentioned cell proliferation factors respectively, inoculate them into culture flasks, add 10-15% FBS (fetal bovine serum) to the MEM medium, and inoculate them under 5% CO 2 , cultured at 37°C for about 10 days. Cells were then detached from the culture flasks by trypsinization and subsequently seeded into bulk porous ceramics formed from β-TCP. As a medium, MEM medium supplemented with 10 to 15% of FBS (fetal bovine serum) was used.
[0046] When sowing, the sowing method of each cell proliferation factor adopts the method of sowing under reduced pressure, sowing und...
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