K-carrageenan oligosaccharide monomer and its preparation method

Abstract

A k-carragheenin oligose monomer C18+12nH28+17n O21+12nS2+nNa2+n, where n=2-11, is prepared from k-carragheenin through degrading by acid, neutralizing by alkali, concentrating, depositing in alcohol, washing and separating by superdex 30 column using the solution of ammonium hydrogen carbonate as flowing phase. Its advantages are simple process and high efficiency.

Description

technical field [0001] The invention relates to a K-carrageenan oligosaccharide, in particular to a K-carrageenan oligosaccharide monomer with a polymerization degree of 7-25 and a preparation method thereof. Background technique [0002] Carrageenan is the cell wall polysaccharide of certain red algae, which is a galactan sulfate ester composed of galactose and galactose derivatives, in which K-carrageenan is a 1,3-linked 4-sulfate group-β-D - Polymers of galactose linked alternately with 1,4-linked 3,6-endidether-α-D-galactose. Because carrageenan is a natural polyanionic sulfated polysaccharide, it is widely used in the fields of medicine, food, agriculture and daily chemical industry. In recent years, many important biological functions and physiological activities of carrageenan oligosaccharides and their derivatives have been gradually recognized by people, and they have shown good applications in antibacterial, antiviral, antitumor, anticoagulant and antiulcer, etc. ...

AI Technical Summary

Problems solved by technology

However, in the current technology and patents for the degradation and preparation of carrageenan oligosaccharides, the mixture of oligosaccharides is obtained, which is not conducive to further in-depth research on the structure and activity of oligosaccharides, that is, the structure-activity relationship, and is not conducive to the study of K-carrageenan from the molecular level. The relationship between gum oligosaccharides and their derivatives and the occurrence and development of diseases and their mechanism of action can guide the research and development of new drugs. Therefore, the preparation of K-carrageenan oligosaccharide monomers is very necessary

However, if the method of chemical synthesis is used to prepare K-carrageenan oligosaccharide monomers, especially the synthesis of oligosaccharide monomers with a degree of polymerization (Dp)>10 or more, because it involves the protection of active groups, the connection mode of sugar units and the three-dimensional A series of problems such as configuration, its technical difficulty and synthesis cost are unacceptable

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