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Method for producing biological protein molecular chips

A manufacturing method and protein technology, applied in the field of biosensing, can solve the problem of low integration, and achieve the effect of improving the firmness and ensuring the activity

Inactive Publication Date: 2004-09-15
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional photolithography technology, the minimum size of the pattern etched on the silicon surface can only be increased by a few hundred nanometers to a few microns, and its integration level is not high, and it needs to be further improved by 1 to 2 orders of magnitude to meet the requirements of today's technology. on request

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  • Method for producing biological protein molecular chips

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Effect test

Embodiment 1

[0018] Embodiment 1: the concrete processing steps of the present invention are as follows:

[0019] a. First, standard semiconductor technology is used to clean and thermally oxidize the silicon wafer; the surface of the cleaned and thermally oxidized silicon wafer has a layer of 500 nanometer silicon dioxide layer;

[0020] b. immerse the above-mentioned pretreated silicon chip in octadecyltrichlorosilane (OTS) for 10 minutes, so that the surface of the silicon chip has octadecyl groups, and octadecyl groups have repellency to protein molecules;

[0021] c. Adopt atomic force microscope (AFM) nano-etching technology to etch nano-patterns on the above-mentioned processed silicon wafer surface; the etching method is: apply a voltage between the atomic force microscope probe and the silicon surface, so that Atoms are oxidized into silicon dioxide; the probe is moved, etched into a square array with a side length of 50 nanometers, and the surface octadecyl group where the etched...

Embodiment 2

[0024] Embodiment two: the concrete processing steps of the present invention are as follows:

[0025] a. First, standard semiconductor technology is used to clean and thermally oxidize the silicon wafer; there is a silicon dioxide layer of 800 nanometers on the surface of the cleaned and thermally oxidized silicon wafer;

[0026] b. immerse the above-mentioned pretreated silicon chip in octadecyltrichlorosilane (OTS) for 10 minutes, so that the surface of the silicon chip has octadecyl groups, and octadecyl groups have repellency to protein molecules;

[0027] c. Adopt atomic force microscope (AFM) nano-etching technology to etch nano-patterns on the above-mentioned processed silicon wafer surface; the etching method is: apply a voltage between the atomic force microscope probe and the silicon surface, so that Atoms are oxidized into silicon dioxide; the probe is moved and etched into concentric circles with a radius of 200 nanometers and 400 nanometers and a line width of 50...

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Abstract

Substantially, the invention is a method for active assembling protein molecules on surface of silicon, applicable to preparation of DNA chip and protein chip. Characters of the invention are as follows. First, silicon surface is treated by using octadecyl trichlorosilane. Next, graph in nano size is etched on silicon surface by using nano etching technique of atomic force microscope (ATM). Then, silicon surface is treated by using gamma - amido - propyl - triethyl silane (gamma-APTES). Finally, the silicon slice with prepared Nano graph is immersed into solution of protein. Thus, protein molecules are fixed on not etched silicon surfaces, and there is no protein molecule on the etched silicon surface. Advantages of the invention are: ensuring active of protein molecules effectively, raising combinative firm degree and superhigh integration level of protein molecules.

Description

technical field [0001] The invention relates to a method for manufacturing a biological protein molecular chip, which is essentially an active assembly method of protein molecules on a silicon surface. The method can be applied to the manufacture of DNA chips and protein chips, and belongs to the technical field of biosensing. Background technique [0002] A DNA chip or a protein chip integrates a variety of DNA or protein activities on a very small geometric surface area. With only a small amount of biological (physiological) sampling, different molecules, including the interaction between molecules and gene expression, can be detected and studied at the same time, and the conditional changes of molecular groups under various conditions can be obtained, so that the laws of life activities can be obtained. Biomolecular chips and biomolecular recognition and detection technology have now become the frontier technology of biomedical engineering in the 21st century. [0003] A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/50G01N33/543G01N33/68
Inventor 焦正吴明红施利毅雷红
Owner SHANGHAI UNIV
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