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Method for single molecule fluorescent sample quick longitudinal super resolution

A single-molecule, super-resolution technology, applied in fluorescence/phosphorescence, analytical materials, material excitation analysis, etc., can solve problems such as unsuitable single-molecule detection

Inactive Publication Date: 2005-03-02
SHANGHAI INST OF OPTICS & FINE MECHANICS CHINESE ACAD OF SCI
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Problems solved by technology

This reconstructed mathematical model uses top-hat mathematical functions or rectangular functions to simulate the longitudinal distribution of fluorescent molecules, but these models actually describe the situation where the number of fluorescent molecules is large and aggregates to form a thin layer structure, which is not applicable to The case for single-molecule detection

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  • Method for single molecule fluorescent sample quick longitudinal super resolution
  • Method for single molecule fluorescent sample quick longitudinal super resolution
  • Method for single molecule fluorescent sample quick longitudinal super resolution

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Embodiment Construction

[0063] See figure 1 with figure 2 , figure 1 It is a schematic diagram of the structure of the total internal reflection fluorescence detection system used in the present invention. The total internal reflection fluorescence detection system consists of a light source 1, an optical fiber 2, an optical fiber precision translation frame 3, a collimating objective lens 4, a convergent lens 5, a two-color beam splitter 6, and imaging An objective lens 7, a stage, and a charge coupled device (CCD) 10 for recording fluorescence imaging are composed. The light source 1 is an Ar ion laser, the mode is TEM00, and the wavelength is 488nm; the fiber 2 is a single-mode polarization-maintaining fiber with a numerical aperture of 0.4; the imaging objective lens 7 is 100×, the numerical aperture is 1.65, oil immersion; the immersion oil 8 has a refractive index of 1.78; The sample stage 9 is spin-coated on the glass slide, and the refractive index of the glass slide is 1.78.

[0064] The meth...

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Abstract

A single molecule fluorescence sample quick longitudinal super resolution method uses total internal reflection fluorescence detecting system. Depth of penetration of exciting field is changed by entrance angle of laser beam being changed in detecting course, according to functional relationship of imaging intensity of single molecule fluorescence having relationship with depth of penetration depth, information of longitudinal interval between fluorescence molecule, absolute longitudinal position and diameter of that are rebuilt. The invention can realize nanometer magnitude longitudinal super resolution of fluorescence molecule, has simple and fast operation and excitation and detection having no touch with sample, can detect sample of solid state and under liquid circumstance and obtain several parameters with once measurement.

Description

Technical field [0001] The invention relates to the detection of single molecules and single atoms. It is a method for rapid longitudinal super-resolution of single-molecule fluorescent samples. In more detail, it is a microscopic method using near-field excitation and full-field detection to realize rapid longitudinal direction of single-molecule fluorescent samples. Super-resolution method. Background technique [0002] The detection and manipulation of single molecules and single atoms has long been a dream of mankind. In the 1990s, with the rapid development of ultra-sensitive detection equipment, single-molecule detection has become possible. It provides scientists with a new means to study cutting-edge topics in fields such as molecular biology, chemistry, medicine, and nanoscience. Before the development of single-molecule detection technology, all molecular experiments were the result of detecting the group average of molecules. Although this type of research has brought ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N13/00G01N21/64
Inventor 王琛刘力王桂英徐至展
Owner SHANGHAI INST OF OPTICS & FINE MECHANICS CHINESE ACAD OF SCI
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