Method for single molecule fluorescent sample quick longitudinal super resolution

Abstract

A single molecule fluorescence sample quick longitudinal super resolution method uses total internal reflection fluorescence detecting system. Depth of penetration of exciting field is changed by entrance angle of laser beam being changed in detecting course, according to functional relationship of imaging intensity of single molecule fluorescence having relationship with depth of penetration depth, information of longitudinal interval between fluorescence molecule, absolute longitudinal position and diameter of that are rebuilt. The invention can realize nanometer magnitude longitudinal super resolution of fluorescence molecule, has simple and fast operation and excitation and detection having no touch with sample, can detect sample of solid state and under liquid circumstance and obtain several parameters with once measurement.

Description

Technical field

[0001] The invention relates to the detection of single molecules and single atoms. It is a method for rapid longitudinal super-resolution of single-molecule fluorescent samples. In more detail, it is a microscopic method using near-field excitation and full-field detection to realize rapid longitudinal direction of single-molecule fluorescent samples. Super-resolution method. Background technique

[0002] The detection and manipulation of single molecules and single atoms has long been a dream of mankind. In the 1990s, with the rapid development of ultra-sensitive detection equipment, single-molecule detection has become possible. It provides scientists with a new means to study cutting-edge topics in fields such as molecular biology, chemistry, medicine, and nanoscience. Before the development of single-molecule detection technology, all molecular experiments were the result of detecting the group average of molecules. Although this type of research has brought ...

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