Microencapsulated saccharomyces and its application

A technology of yeast and microencapsulation, applied in the direction of microcapsules, fungi, digestive system, etc., can solve the problems of no biological activity, no secretion, and acceptance by patients with odor, and achieve the effect of improving cell survival rate

Inactive Publication Date: 2005-03-09
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] First, in the selection of strains, Escherichia coli as the host bacteria of intestinal cell microreactors limits the scope of application of microreactors
Because: ① Escherichia coli is a prokaryotic cell, most of the expressed protein products are gathered in the cytoplasm in the form of inclusion bodies and cannot be secreted outside the cell; ② The protein products expressed in E. coli cannot be modified after transcription, and often have no biological activity; ③Escherichia coli, as the host cell of genetically engineered bacteria, cannot express lo...

Method used

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  • Microencapsulated saccharomyces and its application
  • Microencapsulated saccharomyces and its application
  • Microencapsulated saccharomyces and its application

Examples

Experimental program
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Effect test

example 1

[0022] Example 1: APCA microcapsules containing yeast were prepared under physiological conditions. During the preparation process, the survival rate of yeast was 100%, and the yeast content was 10% 8 One / ml microcapsules, the particle size of the microcapsules is precisely controllable in the range of 100-1000μm.

example 2

[0023] Example 2: After the APCA microcapsules containing yeast were treated with simulated gastric and intestinal fluids, the microcapsules remained intact without damage (see image 3 ).

example 3

[0024] Example 3: After the APCA microcapsules containing yeast are treated with simulated gastric and intestinal fluid, the physiological breaking solution dissolves the microcapsules and releases the yeast. The number of surviving yeast is measured by the dilution plate method, and the yeast count is compared with that before untreated Compared with free yeast, the survival rate of cells in the capsule is increased by more than 200 times.

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Abstract

A yeast in the form of micro capsule for controlling the release of protein medicine to treating uremia, hepatal failure, phenylketonuria, cancer etc has the sodium alginate-polylysine-chitosan structure. The yeast is dispersed in the liquid contained in the microcapsule.

Description

technical field [0001] The invention relates to a microencapsulated yeast that can grow, metabolize and secrete genetically engineered medicine in the intestinal tract. [0002] The present invention also relates to the application of the above-mentioned microencapsulated yeast in treating diseases. Background technique [0003] Genetic engineering drugs make it possible to cure many diseases such as tumors, immunodeficiency diseases, and genetic defect diseases [Document 1: Pennisi E. Breakthrough of the year. Genomics comes of age. Science, 2000, 290 (5500): 2220-2221], [Document 2: Peltonen L, McKusick VA. McKusick. Genomics and medicine. Dissecting human disease in the postgenomic era. Science, 2001, 291(5507): 1224-1229]. However, there are still some problems in the use of genetic engineering drugs at present, such as the extraction of genetic engineering drugs from the metabolites of genetically engineered bacteria (Escherichia coli or yeast), the separation and puri...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61P1/16A61P5/00A61P13/00A61P25/00A61P35/00A61P43/00C12N1/16
Inventor 马小军于炜婷雄鹰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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