Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Red cashew nut legumin and its preparation and use

A technology of kidney and soy bean, applied in the field of separation and purification of plant glycoproteins

Inactive Publication Date: 2005-03-16
广州白云山拜迪生物医药有限公司
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] CN1439425 discloses a kind of phytohemagglutinin (PHA-P), which is used to prepare anticancer drugs. PHA-P is a kind of glycoprotein with a sugar content of 23.1%; but the above-mentioned literature reports are all laboratory Research, phytohemagglutinin has not yet become an industrial pharmaceutical product, it is used clinically

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Red cashew nut legumin and its preparation and use
  • Red cashew nut legumin and its preparation and use
  • Red cashew nut legumin and its preparation and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Embodiment 1: the preparation of red kidney bean element

[0117] Get the red kidney bean seeds produced in Jiangmen, Guangdong, wash and dry, pulverize with a pulverizer, pass through a 50-mesh mesh sieve, weigh 500 grams, extract 12 hours with 5 times the weight of 1% sodium chloride solution, and centrifuge at 3000rpm for 15 hours. Take the supernatant in 10 minutes, precipitate with 70% ethanol, and then precipitate with 10% ether. Take the supernatant and centrifuge at 3000rpm for 15 minutes to get the precipitate, which is about 25g of the crude extract of red kidney bean element. After vacuum freeze-drying, each time After dissolving with 10g coarse powder plus 2% sodium chloride solution, carry out DEAE-Sepharose ion exchange separation after ultrafiltration with a 30kD ultrafiltration membrane, balance the ion exchange column with 15mmol / L pH6.5 phosphate buffer (A liquid), After sample injection, liquid A and liquid B (a solution containing 1 mol / L NaCl in liq...

Embodiment 2

[0166] Get the red kidney bean seeds from Guangdong, wash and dry, pulverize with a pulverizer, pass through a 50-mesh sieve, weigh 400 grams, extract with 7 times the weight of 1% sodium chloride solution for 16 hours, and centrifuge at 3000rpm for 15 minutes Take the supernatant, precipitate with 70% ethanol, and then precipitate with 20% ether, take the supernatant and centrifuge at 3000rpm for 15 minutes to get the precipitate, which is about 20.3g of the crude extract of red kidney bean. After vacuum freeze-drying, each Once the crude extract 10g is dissolved with 1% sodium chloride solution, and after ultrafiltration with 30kD ultrafiltration membrane, carry out DEAE-Sepharose ion exchange separation, with 15mmol / L P H6.5 phosphate buffer (solution A) equilibrates the ion exchange column. After sample injection, liquid A and liquid B (a solution containing 1mol / L NaCl in liquid B) are eluted with a gradient of 0.01-0.05mol / L NaCl , the flow rate is 5ml / min, the detection...

Embodiment 3

[0169] Get the red kidney bean seeds from Guangdong, wash and dry, pulverize with a pulverizer, pass through a 50-mesh sieve, weigh 400 grams, extract 16 hours with 8 times the weight of 1% sodium chloride solution, and centrifuge at 3000rpm for 15 minutes Take the supernatant, precipitate with 70% ethanol, and then precipitate with 30% ether, take the supernatant and centrifuge at 3000rpm for 15 minutes to get the precipitate, which is about 20.3g of the crude extract of red kidney bean element. After vacuum freeze-drying, the 10g crude extract was dissolved with 5% sodium chloride solution and then subjected to DEAE-Sepharose ion exchange separation after ultrafiltration with 30kD ultrafiltration membrane, with 15mmol / L P H6.5 phosphate buffer (liquid A) equilibrates the ion exchange column. After sample injection, liquid A and liquid B (a solution containing 1mol / L NaCl in liquid B) are washed with a gradient of 0.01-0.05mol / L NaCl. The flow rate is 5ml / min, the wavelength ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses red cashew nut legumin, its preparation and use thereof, wherein the preparation comprises disintegrating the red cashew nuts, leaching with 1% sodium chloride solution, extracting the supernatant fluid through centrifuging, settling with alcohol ether mixed liquid, centrifuging to obtain crude extract, vacuum freeze drying and dissolving with sodium chloride, passing through diethylin ethyl cellulose ion exchange chromatography columns, collecting eluent, and vacuum freeze drying.

Description

technical field [0001] The invention belongs to the field of pharmacy, and relates to the separation and purification technology and application of plant glycoprotein extracted from plant seeds. Background technique [0002] Rigas et al. first isolated phytohemagglutinin extract from kidney bean by ammonium sulfate precipitation technology (Rigas D.A. et al., 1955, J. Biol. Chem. 212: 609), but the extract was relatively thick. Allen et al. first separated the phytohemagglutinin extract into L-PHA (leukoagglutinin) which stimulated lymphocyte division and low erythrocyte agglutination activity and L-PHA which stimulated lymphocyte division and erythrocyte agglutination activity by ion exchange chromatography. Protein mixture H-PHA (Allen, L.W., et al., 1969, Proc Natl. Acad. Sci. USA, 63:334). According to the different agglutination activities of PHA, Dahlgren et al. isolated L-PHA that only agglutinates leukocytes and E-PHA that agglutinates both l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P7/00A61P7/06A61P37/04C07K14/415
Inventor 张晓元李素芬周艳霞丘力功
Owner 广州白云山拜迪生物医药有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com