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Tissure culture and fast growth of buttercup flowers

A technology for tissue culture and ranunculus, applied in the field of tissue culture and reproduction of plants, can solve the problems of low proliferation rate, difficulty in large-scale production, difficulty in disinfection and establishment of aseptic systems, etc., and achieves strong proliferation rate and low mutation rate. Effect

Inactive Publication Date: 2005-05-11
云南锦苑花卉产业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the point of view of practical operation, the difficulty of tissue culture of ranunculus ranunculus is manifested in: since there are no obvious axillary buds and stem tips in the growth stage, and the buds that have just germinated from the soil are tender and dirty, it is difficult to disinfect and establish an aseptic system. After the aseptic system is established, the proliferation rate is low, and it is difficult to carry out large-scale production in a short time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Pregermination of bulbs: Soak the bulbs in clean water for 8 hours to make the bulbs fully absorb water and swell, and then sow them in the perlite in the plastic box, and sow them in order according to the order of a layer of perlite and a layer of bulbs , and cover with plastic film to keep moisture, and the water content of the matrix is ​​controlled at 25%. Carry out germination cultivation at 10-12°C. After 13 days, the buds begin to germinate. When the buds grow to 1 cm and appear conical but have no leaves, remove the buds from the base of the bulbs. After the buds are removed, the bulbs can continue to be buried. Re-germination in the substrate for repeated germination;

[0039] 2. Sterilization of explants: The buds are sterilized by a three-step disinfection method. First, the surface of the buds is sterilized in 75% alcohol for 30 seconds, and then the buds are sterilized in 0.15% Hgcl. 2 Sterilize in 1.5% sodium hypochlorite solution for 20 minutes, and ...

Embodiment 2

[0064] Except that the medium is the following medium, all the other steps are the same as in Example 1:

[0065] In induction medium:

[0066] MS medium (same as before)

[0067] BA 0.5mg / L,

[0068] NAA 0.5mg / L,

[0069] Sugar 40g / L,

[0070] Agar 5.5g / L

[0071] Proliferation medium:

[0072] MS medium (same as before)

[0073] BA 0.8mg / L

[0074] NAA 0.1mg / L

[0075] Sugar 30g / L,

[0076] Agar 5.5g / L

[0077] On rooting medium:

[0078] MS medium (same as before)

[0079] BA 0.4mg / L

[0080] NAA 0.1mg / L

[0081] Sugar 30g / L

[0082] Agar 5.5g / L.

[0083] The present invention researches through experiment, and its result report is as follows:

[0084] 1. Materials and methods The materials used are the bulbs of ranunculus variety "2059" imported from the Flower Research Center of the Institute of Horticulture, Yunnan Academy of Agricultural Sciences. Buds, cultivated axillary buds and flower buds were used as explants for tissue culture.

[0085] 2, cultu...

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Abstract

A tissue culture and fast reproduction method for Huamaogen includes such steps as getting its seedball, germinating, sterilizing explant, induced culturing of adventitious buds, secondary culturing of adventitious buds, rooting culturing and transitional transplanting. Its advantages are high efficiency and high speed.

Description

technical field [0001] The invention relates to a plant tissue culture and propagation method, in particular to a ranunculus tissue culture and rapid propagation method. Background technique [0002] Ranunculus is a perennial herbaceous plant of the family Ranunculaceae, the genus Ranunculus, with multiple spindle-shaped roots, solitary or few-branched stems, hairy, few obvious axillary buds, and flower buds growing between leaf axils. Because of its bright colors, rich colors, plump flower shapes, and long vase life, it has achieved good results in the domestic flower market in recent years. However, the current source of provenance is still only imported and the import price is expensive. The conventional propagation method of ranunculus is to use seeds and branch propagation, but because ranunculus is a cross-pollination plant, the hybrid offspring of conventional seed propagation is seriously separated and the traits are unstable, so seed propagation is mainly used in th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 吴丽芳熊丽屈云慧王祥宁张素芳杨春梅蒋亚莲
Owner 云南锦苑花卉产业股份有限公司
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