Agaricus blazei bacterium exopolysaccharide preparing process

A production method and the technology of velvet fungus cells, which are applied in the field of edible fungal polysaccharide production, can solve problems such as falsely high output of exopolysaccharides, quality and purity problems, and easy agglomeration, so as to be beneficial to industrial applications, ensure purity, and increase production Effect

Inactive Publication Date: 2005-06-29
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Agaricus blazei can not only produce a large amount of mycelia during the fermentation process in the culture medium, but also secrete and accumulate exopolysaccharides in the culture medium. In order to obtain this part of exopolysaccharides, we use the above-mentioned starch raw materials for cultivation Based on experiments, it was found that after high temperature sterilization, it is easy to agglomerate or ball, resulting in the formation of Agaricus blazei mycelium balls containing starch granules; In addition to the β-glucan, there is also some starch polysaccharide (raw material) that is α-glucan, which leads to a falsely high yield of exopolysaccharide, and the quality and purity become a problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Agaricus brasiliensis strain: Agaricus brasiliensis 03, which was isolated from Agaricus brasiliensis fruiting bodies grown by mushroom farmers in Curitiba, Paraná, Brazil. Inoculate it on PDA (Potato Dextrose Agar) slant medium, culture it at a constant temperature of 25-28°C for 7-10 days, and store it in a refrigerator at about 4°C for later use. The strain is inoculated on PDA slant medium before use Upper activation, that is, after 7-10 days of constant temperature cultivation at 25-30°C, the mycelium (without medium) was inoculated in a sterilized Erlenmeyer flask containing liquid seed medium and cultured statically for 5 days. The liquid seed medium The formula is (weight %): glucose 2%, yeast extract 0.2%, K 2 HPO 4 0.2%; MgSO 4 0.05%, the balance is water, pH6, and the culture temperature is 28-30°C. At this time, the mycelium grows white and vigorous. Use a sieve to filter and collect the mycelium in the liquid. Wash the bacteria 3 times with water, put th...

Embodiment 2

[0025] The formulation of the production medium in this example is: 50 grams of sucrose, 8 grams of yeast extract and ammonium sulfate 1:1 mixture, K 2 HPO 4 5 g, MgSO 4 2.5 grams, with the balance being water, was prepared into 1000 milliliters of culture solution, and the pH was adjusted to 6. All the other steps from strain preparation to extraction and purification were the same as in Example 1. The exopolysaccharide extraction yield of this example was 789.5mg / L.

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PUM

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Abstract

Disclosed is a method for producing polysaccharide, which has advanced the preparation to produce culture medium, and techniques of fermentation and abstraction, including manufacture of deformation, preparation of seed culture medium and inoculation, production of culture medium for inoculation, abstraction and purification of culture medium. It has increased the quality and purity of the productivity made its productivity to be 825.2mg/L; it has simple technique, low cast and can be applied in industry widely.

Description

technical field [0001] The invention relates to the field of production of polysaccharides from edible fungi, in particular to a method for fermenting, culturing and extracting exopolysaccharides from Agaricus blazei. Background technique [0002] Agaricus blazei, also known as Brazil mushroom, is a very rare fungus with both medicinal and edible functions, and its scientific name is A. brasiliensis. Agaricus blazei mushrooms are rich in nutrition, delicious in taste, and have a strong almond fragrance. Each 100 grams of Agaricus blazei dry product contains 40-45 grams of crude protein, 38-45 grams of sugar, 6-8 grams of fiber, and 3-4 grams of crude fat , is an edible fungus rich in protein and sugar; it is also rich in vitamins, such as VB 1 0.3-0.4 mg, VB 2 3.2-3.7 mg, niacin 22-50 mg, ergosterol 100-200 mg (VD original). The bacterium contains 18 kinds of amino acids, of which the essential amino acids for human body account for 39.7-42.8% of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P19/04
Inventor 范雷法潘慧娟
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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