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Process for the production of methionine

A technology of methionine and amino group is applied in the field of preparation of methionine crystal, which can solve the problems of low solubility, difficult recovery of biocatalysts, and inability to increase the dissolved accumulation of methionine.

Inactive Publication Date: 2005-09-21
NIPPON SODA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the process of using these nitrilase-producing bacteria to hydrolyze 2-amino-4-methylthiobutyronitrile as a biocatalyst to obtain methionine, the following problems are faced: the solubility of methionine relative to water is low (25 3.38g / 100g·H at ℃ 2 O, 6.07g / 100g·H at 50°C 2 (2), so, just after the reaction starts, there is methionine crystallization in the reaction solution, which coagulates with the biocatalyst, and it is difficult to effectively separate the three components of methionine precipitation crystal, biocatalyst and reaction solution, and it is difficult to reclaim the biocatalyst with a high recovery rate.
On the other hand, it has been found that during the reaction, the biocatalyst can be separated and recovered by keeping methionine from being dissolved in the reaction liquid (water) without precipitation, but in this case, methionine has a low solubility with respect to water, so it must be Maintaining the accumulated concentration of methionine at an extremely low concentration of less than 5% cannot increase the dissolved and accumulated amount of methionine in the reaction solution, which is a manufacturing technique with extremely low productivity and lacks practicality
In order to dissolve about 20% by weight of methionine in water, inorganic bases such as NaOH or KOH can also be added. In this case, when obtaining methionine as a solid free product, it is necessary to neutralize it with an acid. At this time, a large amount of Inorganic salt waste, there is a problem that additional operations to remove these wastes have to be performed

Method used

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  • Process for the production of methionine
  • Process for the production of methionine

Examples

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preparation example Construction

[0024] The preparation method of methionine of the present invention is not particularly limited as long as it has the following steps, that is, (1) the first step: using a biocatalyst with hydrolysis activity in an ammonia solution to decompose a raw material that can be hydrolyzed to methionine Carry out hydrolysis, preferably utilize the biocatalyst that has nitrile hydrolysis activity to hydrolyze raw material 2-amino-4-methylthiobutyronitrile in ammonia solution or utilize the biocatalyst that has amide hydrolysis activity to 2-amino- 4-Methylthiobutyramide is hydrolyzed to be converted into an ammonia solution containing methionine; (2) second process: the ammonia solution containing methionine obtained in the aforementioned first process is separated from the biocatalyst; (3) the third process : Ammonia is distilled off from the methionine-containing ammonia solution separated in the second step, and methionine is crystallized and separated. For example, if figure 2 A...

Embodiment 1

[0036] Hereinafter, although an Example demonstrates in more detail, this invention is not limited to these Examples. Example 1 (Preparation of DL-Methionine by NSSC204 Strain) (Cultivation of NSSC204 Strain)

[0037] Containing 0.5% of yeast extract, 0.5% of glucose, 0.1% of dipotassium hydrogen phosphate, 0.1% of potassium dihydrogen phosphate, 0.1% of common salt, 0.02% of magnesium sulfate heptahydrate, 0.001% of ferrous sulfate and 0.03% of 2-aminobenzonitrile 2ml of the culture medium was placed in a test tube and sterilized at 121°C for 20 minutes. One Arthrobacter strain NSSC204 of Platinum auricularia was implanted in the test tube, and cultured with shaking at 33° C. overnight to prepare a pre-culture. Next, 2.0% of corn steeping liquid (sterilized by filtration), 1.0% of sucrose (sterilized at 121°C for 20 minutes), 0.03% of 2-aminobenzonitrile (sterilized at 121°C for 20 minutes), pH 7.2 (with 2N Caustic soda adjusted) medium 20ml was added to a 100ml Erlenmeyer ...

Embodiment 2

[0040] Embodiment 2 (continuous production of DL-methionine by NSSC204 strain)

[0041] The culture solution of the Arthrobacter NSSC204 strain obtained in Example 1 was centrifuged, washed with ion-exchanged water, and then suspended in an aqueous solution containing 10% (W / W) DL-methionine and 2.28% (W / W) ammonia. In the aqueous solution (pH9.5), the concentration is 2% (W / W) in terms of dry cells. 300 g of this bacterial cell suspension was added to a three-neck flask with a capacity of 500 ml maintained at a temperature of 30° C., and 2-amino-4-methylthiobutyronitrile was continuously added at a rate of 5.5 g per hour while stirring. In addition, bacterial cells were continuously filtered using a microfiltration membrane (microza PMP-003 manufactured by Asahi Kasei), and the reaction filtrate was recovered at a rate of about 54 g per hour. At this time, in order not to reduce the amount of liquid in the reaction vessel, a pump linked to the liquid level sensor was used to...

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Abstract

A practical method for producing methionine is presented. When a substance capable of producing methionine through hydrolysis is used as raw material and converted into methionine by using a biocatalyst, the biocatalyst is repeatedly usable and the accumulation amount of methionine dissolved in the reaction solution is increased, and methionine is obtained in a solid form from the reaction solution. Specifically, the present invention relates to a method for producing methionine comprising the following steps: a first step of converting raw material capable of producing methionine through hydrolysis of 2-amino-4-methylthiobutyronitrile, and 2-amino-4-methylthiobutanamide or the like in an aqueous ammonia solution, into methionine-containing aqueous ammonia solution, with a biocatalyst through hydrolysis, a second step of separating the biocatalyst from the methionine-containing aqueous ammonia solution obtained in the first step, and a third step of distilling ammonia out of the methionine-containing aqueous ammonia solution separated in the second step to deposit and separate crystals of methionine.

Description

technical field [0001] The present invention relates to a method for preparing methionine crystals used as pharmaceuticals or feed additives. Methionine is required especially in the feed additive market in solid (crystalline) form. Background technique [0002] As a method for preparing 2-amino acids utilizing the nitrile hydrolysis activity of a biocatalyst, a method using 2-aminonitrile or 2-aminoamide as a raw material is known (Japanese Patent Publication No. 58-15120, Japanese Patent Publication No. 63-500004 , JP-2-31694, JP-3-500484, JP-3-16118, WO02 / 08439, JP-2002-34593, US-6417395) and a method using cyanohydrin as a raw material (JP Kaiping 9-140391). However, neither method discloses a practical method for efficiently separating and purifying a solid 2-amino acid having low water solubility such as methionine from a biocatalyst. [0003] In addition, in the microbiological manufacture of glycine from glycylnitrile obtained by the reaction of formaldehyde, hydr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/12
CPCC12P13/12
Inventor 小林洋一小野逸平早川公一水井良典石川高广
Owner NIPPON SODA CO LTD
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