Human vaccine containing bee glue adjuvent and its preparing method
A vaccine preparation and glue adjuvant technology, applied in the field of biological drugs, can solve the problems of lack of biological drugs, development and application in the field of biochemistry, limited application of propolis, etc., and achieve the effect of no toxic side effects, safe and reliable chemical residues, and no chemical residues
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[0049] Example 1. Human vaccine containing propolis adjuvant, influenza virus containing propolis adjuvant, 5 mg per milliliter containing propolis adjuvant; according to the nanoscale of influenza virus vaccine immunologically active antigen, it is 5 nanometers larger than immunological active antigen.
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[0050] Example 2: Crude propolis → freezing → crushing → ethanol extraction → filtrate → activated carbon filtration → continuous centrifugation → removal of activated carbon → removal of precipitates → addition of vaccines → nanotechnology treatment → ultrafiltration → vacuum concentration → human vaccines containing propolis adjuvants → freezing Store and save.
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[0051] Example 3. Take 10000 ml of influenza whole virus or nano-split influenza virus or nano-split influenza virus after hydrostatic pressure inactivation, and add 10000 ml of propolis adjuvant to each ml vaccine containing propolis 5 Milligrams and mix thoroughly, and then according to the nano-scale size of each vaccine immunologically active antigen, generally 5 nanometers larger than the immunologically active antigen is selected, and the adjusted nano collider is used for nano-technology processing to make the processed high-speed fluid particles instantaneously split, The functions of pulverization, dispersion, emulsification, catalysis and synthesis are integrated to further improve the immunogenicity and antiseptic function of propolis as a vaccine immune adjuvant to ensure the safety and stability of the vaccine.
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