Chitin contained medical nerve graft and its preparation method

A technology of nerve transplantation and chitin, applied in the field of medical nerves, can solve the problems of uncontrollable, high protein content, limited application, etc., and achieve good results

Active Publication Date: 2005-10-26
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the degradation rate of the neural tissue engineering catheter prepared by pure chitosan is not ideal and cannot be controlled, which limits its wide application.
Chitin (1) Currently, chitin is desalted with dilute acid and protein with dilute alkali, so the impurities in chitin, especially the protein content, are relatively high, which will cause severe inflammatory reactions when used in the human body; (2) chitin dissolves Poor performance, only soluble in some organic solvents of halogenated hydrocarbons, so it is difficult to process; (3) The degree of acetylation of chitin is difficult to change, which limits its application in this area
Therefore so far, there is no artificial nerve graft prepared by chitin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] ① Chitosan is prepared into a chitin-containing catheter: the chitosan is dissolved with a weak acid, the weak acid is acetic acid (or phosphoric acid, citric acid, lactic acid), and the concentration is 2 to 15% (example 2%, 8%, 15% ), then the solution is passed through a tubular forming die, and molded in an alkaline coagulation liquid, which is a solution containing 8-20% sodium hydroxide (or 8-20% potassium hydroxide or 8-20% sodium carbonate , can also contain ethanol 5~30% at the same time), after molding, the catheter is cleaned with water, and dried to obtain a chitosan catheter; then the obtained chitosan catheter is put into an alcohol solution of acetic anhydride for acetylation reaction, and the reaction temperature is 0-60°C (for example 0°C, 30°C, 60°C), the reaction time is 0.5-6 hours (for example 0.5 hours, 2 hours, 4 hours, 6 hours), (reaction time and temperature affect the degree of acetylation of chitin , the reaction time is long, the reaction tem...

Embodiment 2

[0026] ① First, chitosan is made into powder by mechanical processing or spray drying, and then the chitosan powder is put into the alcohol solution of acetic anhydride for acetylation reaction. The reaction temperature is 0-60°C (for example, 0°C, 60°C), the reaction time is 0.5 to 6 hours (for example, 0.5 hours, 2 hours, 4 hours, 6 hours). In the alcohol solution of acetic anhydride, the concentration of acetic anhydride is 0.1~50% (for example 0.1%, 20%, 40%, 50%), and the alcohol solution is methanol (or ethanol). After the acetylation reaction, wash with water, then put it into 1mol / L sodium hydroxide or potassium hydroxide solution for 24 hours, wash it with water, and dry to obtain chitin powder with an acetylation degree of 30-100%; another chitosan is used After the weak acid is dissolved into a solution, add the chitin powder obtained above, the weak acid is acetic acid (or phosphoric acid, citric acid, lactic acid), the concentration is 2 to 15% (for example 2%, 8%...

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PUM

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Abstract

A medical nerve-grafting object containing chitin is prepared through preparing the cathether containing chitin from chitosan, preparing the chitin fibre supporter from chitosan, and embedding said supporter in said cathether. Its advantages are high effect, high and controllable degradability and high biocompatibility.

Description

Technical field: [0001] The invention relates to a medical nerve for bridging defective nerves. Background technique: [0002] Clinically, peripheral nerve defects caused by trauma (or surgery) are relatively common, but when long-distance nerve defects cannot be compensated for by end-to-end sutures, grafts have to be used to bridge and repair them. Although it has been more than 100 years to find and develop a more suitable graft, except for the autologous nerve which has become the first choice for bridging the nerve defect, it has not yet found a more ideal, recognized and widely used graft in humans. Clinically widely used nerve graft substitutes. As for autologous nerve transplantation, it has not been widely used clinically because of the limited source of nerves for transplantation, difficulty in matching tissue structure and size, and long-term denervation of the transplanted donor site. [0003] With the emergence and development of tissue engineering, it provide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/02
Inventor 杨宇民顾晓松王晓冬丁斐胡文
Owner NANTONG UNIVERSITY
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