Polymer of PH sensitive type capable of regenerating amphoteric ion for double aqueous phase system and preparation process thereof

A zwitterion and polymer technology, which is applied in the field of pH-sensitive methacrylate polymers and their preparation, can solve problems such as the destruction of biologically active substances, and achieve the effects of maintaining biomass activity and a simple and feasible preparation method.

Inactive Publication Date: 2005-11-09
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since biologically active substances are very sensitive to overheating, extreme pH, and pressure, drastic changes in these conditions will greatly damage biologically active substances

Method used

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  • Polymer of PH sensitive type capable of regenerating amphoteric ion for double aqueous phase system and preparation process thereof
  • Polymer of PH sensitive type capable of regenerating amphoteric ion for double aqueous phase system and preparation process thereof
  • Polymer of PH sensitive type capable of regenerating amphoteric ion for double aqueous phase system and preparation process thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The synthesis of embodiment 1 ABC trimer:

[0026]Add 33.7ml (0.2mol) 2-methylaminoethyl methacrylate, 16.2ml (0.1mol) tert-butyl methacrylate, 5.3ml (0.05mol) methyl methacrylate in a stoppered Erlenmeyer flask, 200ml of tetrahydrofuran was fully dissolved under stirring, azobisisobutyronitrile AIBN of 2% of the total mass of the three monomers was added as an initiator, and nitrogen was filled to remove oxygen for 15 minutes. The reaction vessel was sealed, and the reaction was carried out at 60° C. with shaking in a water bath at 250 rpm for 24 hours. After the reaction was completed, the product was precipitated with petroleum ether, and the precipitate was dried to constant weight to obtain the final product. Its isoelectric point PI=8.4 was determined. The polymer has a UV absorption maximum at 234 nm.

Embodiment 2

[0028] The mass ratio is 5% ABC trimer aqueous solution P ABC Prepare two aqueous phases with a mass ratio of 10% polyethylene glycol 20000 (PEG20000) aqueous solution, P ABC The optimal volume ratio to PEG20000 solution is 3:2, mix well, and centrifuge to promote phase separation. An appropriate amount of 0.1M NaOH aqueous solution was added to the upper and lower phases respectively to adjust the pH value of the solution, so that the polymer was precipitated at the isoelectric point. Centrifuge, take the supernatant and measure P at 234nm ABC The absorbance, the total recovery of the polymer calculated by the standard curve was 94.5%. Theoretically, it can be used more than 40 times.

Embodiment 3

[0030] Prepare a two-phase aqueous system, the upper phase is P ABC (5%w / w) 3ml, the lower phase is PEG20000 (10%w / w) 2ml, add Na 2 SO 4 Solid, the final concentration is 200mM, distribute the solute BSA (bovine serum albumin), the initial concentration is 0.6mg / ml, mix and centrifuge at low speed to promote the formation of two phases, and measure the absorbance of BSA in the upper and lower phases at 280nm , the calculated distribution coefficient of BSA was 12.5. It is proved that the polymer can be used in two-phase extraction.

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Abstract

A pH-sensitive and regeneratable amphionic polymer for the separating system of dual water phases is proportionally prepared from three monomer compounds through atactic copolymerizing. It has high bioactivity.

Description

technical field [0001] The invention relates to a water-soluble polymer used in a two-water phase separation system, in particular to a recyclable and reusable pH-sensitive methacrylate polymer used in a two-water phase separation system and a preparation method thereof. Background technique [0002] The two aqueous phase system generally refers to the two immiscible aqueous phase systems formed by dissolving polymer-polymer or polymer and inorganic salt in water at an appropriate concentration. Because the two-phase system has low interfacial tension, mild separation process, low equipment investment, low energy consumption, simple operation, easy to scale up, and no organic solvent residue, it has great potential in the separation and extraction of biomass and drugs application prospects. However, due to biological cost and environmental reasons, phase-forming polymers need to be recovered and recycled. How to prepare a reversible polymer so that it is suitable for two-p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/34
Inventor 曹学君秦伟
Owner EAST CHINA UNIV OF SCI & TECH
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