Process for preparing notoginseng triol saponin and use thereof

A technology of triol saponin and alcohol saponin, which is applied in the preparation field of extracting notoginseng triol saponin from Panax notoginseng, to achieve the effects of fast absorption, inhibition of thrombus formation, and good color

Active Publication Date: 2005-11-23
李明劲
View PDF0 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] After many years of research, the inventors have found that many ingredients in Panax notoginseng saponins are ineffective in resisting platelet aggregation and preventing thrombosis, and that

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for preparing notoginseng triol saponin and use thereof
  • Process for preparing notoginseng triol saponin and use thereof
  • Process for preparing notoginseng triol saponin and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Example

[0038] Example 1: The chemical composition of notoginseng triol saponins (PTS)

[0039] ①, Notoginsenoside C 1 , D 1 Separation of

[0040] Thin-layer silica gel (10-40μ produced by Qingdao Ocean Chemical Plant) dry packed column (¤42*400mm). PTS is dissolved in dilute alcohol, mixed with thin layer of silica gel, dried at low temperature, and loaded into the top of the column. The lower phase of chloroform-methanol-water (65:35:10) is the developing solvent, which is received by the automatic sampling receiver, each 20ml . TLC identification, spot samples on a high-efficiency thin-layer silica gel plate (produced by Qingdao Ocean Chemical Factory) and unfold it on a horizontal unfolding tank. The unfolding solvent is the same as that of the column chromatography developing agent system. After unfolding, dry it and spray with sulfuric acid ethanol solution. Bake at ℃ for 5 minutes, or hot-air bake with an electric hair dryer, combine the fractions with the same chromatographic spo...

Example

[0103] Example 2: Prepare notoginseng triol saponin according to the following steps:

[0104] A. Take panax notoginseng and crush it through No. 1 sieve, add 60% ethanol and soak for 14 hours, then evenly, compactly fill it into the percolation bucket;

[0105] B. Use 6 times the amount of 60% ethanol to permeate the effluent with no saponin reaction at a flow rate of 3 ml / min per kilogram of medicinal materials. When percolating, the soaking liquid will flow to the full surface first, and then 60% ethanol will be added dropwise. The upper and lower flow rates are equal to each other, and the column cannot be dried;

[0106] C. When the vacuum degree is 0.04Mpa and the temperature is 50-55℃, the percolate is concentrated under reduced pressure, ethanol is recovered, and the concentrated liquid has no alcohol smell, and the relative density is 1.06-1.12;

[0107] D. Add water to 3 times the volume of the concentrated dialysis solution without alcohol, centrifuge, take the clear li...

Example

[0110] Example 3: Prepare panaxatriol saponin according to the following steps:

[0111] A. Take panax notoginseng and crush it through No. 1 sieve, add 90% ethanol and soak for 28 hours, then evenly, compactly fill it into the percolation bucket;

[0112] B. Use 12 times the amount of 60% ethanol to permeate the effluent with no saponin reaction at a flow rate of 7 ml / min per kilogram of medicinal materials. When percolating, the soaking liquid will first flow to the entire liquid surface, and then 60% ethanol will be added dropwise. The upper and lower flow rates are equal to each other, and the column cannot be dried;

[0113] C. When the vacuum degree is 0.08Mpa and the temperature is 50-55℃, the percolate is concentrated under reduced pressure, and ethanol is recovered. The concentrated liquid has no alcohol smell and the relative density is 1.06-1.12;

[0114]D. Add water to 6 times the volume of the concentrated dialysis solution without alcohol, centrifuge, take the clear ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Melting pointaaaaaaaaaa
Login to view more

Abstract

The invention discloses a process for preparing notoginseng triol saponin and use thereof, which includes following steps: crushing the notoginseng, and adding ethanol solution whose concentration is 50 to 90% to immerse and percoalte the notoginseng, and then collecting the percoalted liquid and reducing pressure to concentrate the same, and then using distilled water to dilute the concentrated liquid, and then slowly filling the liquid into the large-hole absorption resin separating column, and using 40% ethanol to elute, and then collecting the 40% ethanol elutriant and refining the same to obtain notoginseng triol saponin. The notoginseng triol saponin extracted by the provided process contains all triol saponin in the notoginseng, wherein contains 156 to 79 % ginsenosiderx Rg, 5.0 to 7.5 % of ginsenosiderx Re, 11 to 14 % triol saponin R1, 78 to 98 % of notoginseng triol saponin. The notoginseng triol saponin can be used in treatment for cardiocerebrovascular embolic disease, and has advantages including high content, stable quality, good colour, low cost, and its organic solvent residual amount complies with drug standard.

Description

field of invention [0001] The present invention relates to a method for extracting medicinal plants and its application, more particularly to a preparation method for extracting notoginseng triol saponins from Panax notoginseng and its application. Background technique [0002] Panax notoginseng is a traditional Chinese medicine commonly used in traditional Chinese medicine. Its functions are recorded in the Chinese Pharmacopoeia as dispelling blood stasis, stopping bleeding, reducing swelling and relieving pain. [0003] Notoginseng triol saponin is the saponin extract of the single traditional Chinese medicine Panax notoginseng (also known as Tianqi, ginseng notoginseng), which is the effective part of Sanqi to promote blood circulation and remove blood stasis. Saponins Rg1, Re, and notoginseng saponin R1, and a small amount of Rb1, Rd, etc. [0004] Panax notoginseng, also known as Tianqi or Panax notoginseng, is the rhizome of Panax notoginseng (Burk) F.H.Chen of Aralia...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07J9/00
Inventor 李明劲
Owner 李明劲
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap