Unlock instant, AI-driven research and patent intelligence for your innovation.

Sword-like iris vacuolar membrane Na+/H+ antiport protein-2 gene and its application

A technology of antiporter and tonoplast membrane, applied in the field of Na+/H+ antiporter 2 gene

Inactive Publication Date: 2005-12-14
SHANDONG UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the current situation that there is no report on the saline-alkali-tolerant Iris NHX gene and its application in plant transgenic breeding engineering technology, the invention provides a Iris tonoplast Na + / H + Antiporter protein 2 gene, recombinant vector and plant transgenic breeding method, the Sinensis tonoplast Na + / H + The antiporter 2 gene is constructed on the transformed plasmid expressed in plants, and transformed into wheat to obtain transgenic plants

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sword-like iris vacuolar membrane Na+/H+ antiport protein-2 gene and its application
  • Sword-like iris vacuolar membrane Na+/H+ antiport protein-2 gene and its application
  • Sword-like iris vacuolar membrane Na+/H+ antiport protein-2 gene and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0069] 1. Ma Lin Na + / H + Cloning of Partial Fragment of Antiporter 2 Gene

[0070] Trizol method to extract total RNA from 2-week-old Iris seedlings, PowerScript TM The first-strand cDNA was synthesized by reverse transcription with reverse transcriptase, and PCR was carried out with degenerate primers: P1: 5'-GAT TCT GTW TGC ACM YTR CAG GT-'3, P2: 5'-CAT KAG MCC AGC CCA CCA WAT-'3 Amplify, obtain an amplified fragment, clone and sequence.

[0071] Amplify the target gene fragment according to the following conditions:

[0072] Reaction system: 2×GC buffer I 25μl, 10mM dNTP 3μl, 10μM primer 1 and primer 2 1.5μl each, DNA template 250ng, LA GC Taq enzyme 1μl, add water to make up to 50μl.

[0073] The reaction program was: pre-denaturation at 94°C for 3 min, followed by 36 cycles, each cycle including denaturation at 94°C for 40 s, renaturation at 59°C for 1 min, and extension at 72°C for 1 min. Finally, it was kept at 72°C for 10 min.

[0074] 2. Ma Lin Na + / H + Acq...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses the nucleic acid sequence and corresponding amino acid sequence of one kind of sword-like iris vacuolar membrane Na+ / H+ antiport protein-2 gene, and the biotechnological application in improving salt resisting quality of crop and pasture. The sword-like iris vacuolar membrane Na+ / H+ antiport protein-2 gene is one member in sword-like iris vacuolar membrane Na+ / H+ antiport protein family and is related positively to the salt resisting property of sword-like iris. Based on the gene sequence, the gene may be cloned and prokaryotic expression vector may be constituted for expression in prokaryotic cell in great amount to obtain Na+ / H+ antiport protein-2 for protein structure research, function research and antigen preparation. After cloning the gene, various kinds of eukaryotic expression vector may be constituted, and the gene may be led into crop and pasture through different methods to breed excellent salt tolerant variety.

Description

technical field [0001] The present invention relates to the Na + / H + The antiporter 2 gene (IINHX2) belongs to the field of biotechnology. technical background [0002] Strategies for plants to eliminate Na+ toxicity include: reducing Na+ uptake, Na+ efflux and Na+ compartmentalization. Na+ uptake is a complex process. Na+ efflux and compartmentalization are indirect active transport processes, mainly regulated by Na+ / H+ antiporters. Na + / H + Antiporter (Na + / H + Antiporter or exchanger, NHA or NHE) is a transport protein ubiquitous in the membrane system of bacteria, yeast, algae, animals and higher plants, involved in the pH, Na + Life activities such as concentration regulation and cell volume change. Since Blumwald and Plooe first discovered the Na+ / H+ antitransport activity on the tonoplast membrane of sugar beet root storage tissues in 1985, people have successively discovered the Na+ / H+ reverse transport activity in halophytes such as Astringer, facultati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29
Inventor 夏光敏刘恒薛哲勇
Owner SHANDONG UNIV