Calcium ion content determination method and calcium ion diagnosis kit

A technology for diagnostic kits and assay methods, applied in the field of medical inspection and assay, can solve problems such as high cost

Inactive Publication Date: 2006-04-26
王尔中
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

but the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Embodiment one (single dose)

[0077] The ionized calcium diagnostic kit of the present embodiment comprises:

[0078] Buffer 80mmol / l

[0079] Phospholipids 4mmol / l

[0080] Ethanol 2mmol / l

[0081] Oxidized coenzyme 1mmol / l

[0082] Phospholipase (requires calcium ion) 12000U / l

[0083] Choline oxidase 1000U / l

[0084] Catalase 1000U / l

[0085] Aldehyde dehydrogenase 1000U / l

[0086] Stabilizer 50% (total volume)

[0087] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 340nm, test sub-wavelength above 405nm, the volume ratio of the measured ionized calcium sample to the reagent is 1 / 25, and the reaction direction is positive reaction .

[0088] After the sample and reagents are added and allowed to mix, the following reactions occur:

[0089] Phospholipid + water Phospholipase-D / Ca ++ Phosphatidic acid + choline

[0090] Choline + 2 oxygen + water choline oxidase Beta...

Embodiment 2

[0095] Embodiment two (two doses)

[0096] The ionized calcium diagnostic reagent of the present embodiment has:

[0097] Reagent I

[0098] Buffer 100mmol / l

[0099] Ethanol 6mmol / l

[0100] Oxidized coenzyme 3mmol / l

[0101] Choline oxidase 5500U / l

[0102] Catalase 5500U / l

[0103] Aldehyde dehydrogenase 5500U / l

[0104] Stabilizer 50% (total volume)

[0105] Reagent II

[0106] Buffer 100mmol / l

[0107] Phospholipids 7mmol / l

[0108] Phospholipase (requires calcium ion) 60000U / l

[0109] Stabilizer 50% (total volume)

[0110] When measuring the ionized calcium content, the temperature is controlled at 30°C, the reaction time is 15 minutes, the main wavelength of the test is 340nm, the secondary wavelength of the test is above 405nm, the volume ratio of the measured ionized calcium sample to the reagent is 1 / 25, and the reaction direction is positive reaction.

[0111] The specific measurement steps are:

[0112]Phospholipid + water Phos...

Embodiment 3

[0118] Embodiment three (three doses)

[0119] The ionized calcium diagnostic reagent of the present embodiment is three doses, has:

[0120] Reagent I

[0121] Buffer 120mmol / l

[0122] Ethanol 10mmol / l

[0123] Oxidized coenzyme 5mmol / l

[0124] Stabilizer 20mmol / l

[0125] Reagent II

[0126] Buffer 120mmol / l

[0127] Choline oxidase 10000U / l

[0128] Catalase 10000U / l

[0129] Aldehyde dehydrogenase 10000U / l

[0130] Stabilizer 50% (total volume)

[0131] Reagent III

[0132] Buffer 120mmol / l

[0133] Phospholipids 10mmol / l

[0134] Phospholipase (requires calcium ion) 100000U / l

[0135] Stabilizer 50% (total volume)

[0136] Set on the automatic biochemical analyzer: temperature 25°C, reaction time 20 minutes, test main wavelength 340nm, test sub-wavelength above 405nm, the volume ratio of the measured ionized calcium sample to the reagent is 1 / 25, and the reaction direction is positive reaction .

[0137] The specific measurement...

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PUM

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Abstract

The present invention relates to ionic calcium content determining method and ionic calcium diagnosis kit, and belongs to the field of medical detecting technology. The kit includes buffering solution, phospholipid, ethanol, oxidizing coenzyme, phosphatidase, choline oxidase, hydrogen peroxidate, aldehyde dehdrogenase and stabilizer. Through mixing the sample and reagent in certain volume ratio to generate enzyme coupling reaction, detecting the main wavelength absorbance variation of the reaction product under biochemical analyzer and calculation, ionic calcium content is obtained. The present invention has the advantages of high sensitivity, high precision and no contamination of inner and outer matters.

Description

technical field [0001] The invention relates to a method for determining ion calcium content, and at the same time, the invention also relates to an ion calcium diagnosis kit for realizing the method, which belongs to the technical field of medical examination and determination. Background technique [0002] There are many assay methods for total calcium, which can be summed up with titration (comprising oxidation-reduction titration, complexometric titration), colorimetry (the most commonly used and convenient for automatic operation are o-cresolphthalein complex ketone method, methylthymol blue method, arsenazo III method, etc.), flame photometry, atomic absorption spectrophotometry, isotope dilution mass spectrometry. The definitive method for calcium determination recommended by the International Federation of Clinical Chemistry (IFCC) is isotope dilution mass spectrometry (IDMS), and the reference method is atomic absorption spectrophotometry. The routine method recomm...

Claims

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Application Information

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IPC IPC(8): C12Q1/32
Inventor 王尔中
Owner 王尔中
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