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Method of enhancing autoimmunity

An autoimmune and enhancer technology, applied in the direction of pharmaceutical formulations, cosmetic preparations, allergic diseases, etc., to achieve excellent selective effects

Inactive Publication Date: 2006-05-03
NAT INST OF ADVANCED IND SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its highly active hemagglutinin has physiological activities such as enhancing autoimmunity, which have been unknown so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Extraction step of water-soluble fraction

[0042] Tsurushiramo (produced in the Yoshino River Estuary area of ​​Tokushima Prefecture, Japan) was washed with a 0.15 M sodium chloride aqueous solution, and dried in the sun to obtain a dried product. Add 700ml of 100mM phosphate buffer solution (pH6.9) containing 0.15M sodium chloride to 100g of the dried product for homogenization, then place the homogenized solution at 4°C for 6 hours, and obtain the supernatant by centrifugation crude extract of liquid.

[0043] (2) Separation step of crude active ingredient

[0044] Next, ammonium sulfate was added to the crude extract to make the final concentration reach 35% saturation concentration to carry out the first-stage salting-out. After the addition of ammonium sulfate was completed, it was left to stand at a temperature of 4° C. for 1 hour, and the generated precipitate was removed by centrifugation. In this operation, impurities such as pigments are removed as pre...

Embodiment 2

[0058] (1) Extraction step of water-soluble fraction

[0059]A 500-g wet weight of Asparagus (produced in the Yoshino River Estuary area of ​​Tokushima Prefecture, Japan) was washed with a 0.15M aqueous sodium chloride solution, and frozen at -30°C. Using 0.5M tris(hydroxymethyl)aminomethane-hydrochloric acid buffer (pH 8.2) containing 30 mM potassium chloride and 3 μM zinc sulfate, 5 mM 2-mercaptoethanol as the buffer for extraction, to finely crushed frozen seaweed (equivalent to Add 800ml of buffer solution for extraction to Homogenate (wet weight 500g), and then homogenize the homogenized solution at 4°C for 6 hours, then centrifuge to obtain a crude extract of the supernatant.

[0060] (2) Separation step of crude active ingredient

[0061] Next, ammonium sulfate was added to the crude extract to make the final concentration reach 35% saturation concentration to carry out the first-stage salting-out. After the addition of ammonium sulfate was completed, it was left to s...

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PUM

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Abstract

Provides a new method of enhancing autoimmunity by administering a specific active ingredient that does not lose its sugar binding property due to heating, shows good selectivity for recognizing sugar chains, and activates cellular immunity Autoimmune enhancing activity such as ability. The active ingredient is obtained by the following method: in the extract liquid extracted from Gracilaria red algae with saline solution, by adding ammonium sulfate so that the final concentration reaches 20-40% saturation concentration to carry out the first stage of salting out, Remove the precipitated impurities, and then further add ammonium sulfate to make the final concentration reach 60-80% saturation concentration to carry out the second stage of salting out, recover the crude active fraction in the form of precipitation, and obtain liquid crude Active fraction; if necessary, remove impurity proteins by heat treatment at 100°C for 1 to 10 minutes, then separate fractions with a molecular weight of 100,000 or more by gel filtration chromatography, and further separate the components by chromatography, collect and display The active ingredient is obtained by activating the fraction of cellular immunity.

Description

technical field [0001] The present invention relates to a new method of enhancing autoimmunity by administering a special active ingredient, a method for producing the active ingredient, and a cosmetic material compounded with the active ingredient, wherein the special active ingredient is composed of red algae of the genus Gracilaria (Gracilaria sp.) as a raw material, it will not cause its sugar-binding property to disappear due to heat treatment, and it shows good autoimmunity-enhancing activity such as the selectivity of recognizing sugar chains and the activation of cellular immunity. Background technique [0002] Since hemagglutinin exhibits specific behaviors with respect to red blood cells of various animals, it is widely used as a test reagent or a separation material in the fields of medicine, pharmacy, biochemistry, and the like. The hemagglutinin can be broadly classified into animal-derived and plant-derived hemagglutinins, but plant-derived hemagglutinins are a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/02A23L1/30A61K8/97A61K36/04A61P37/04
CPCA23L33/105A61K36/04A61P37/04
Inventor 垣田浩孝上嵨洋
Owner NAT INST OF ADVANCED IND SCI & TECH
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