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Dyeing indicating method used for detecting food poisoning pathogenic bacteria

A pathogenic bacteria detection and toxin technology, applied in the field of pathogenic bacteria detection, can solve the problems of long detection time, high blindness, low detection rate, etc., achieve the improvement of detection efficiency and accuracy, increase the positive detection rate, and shorten the detection time Effect

Inactive Publication Date: 2006-06-28
台州市疾病预防控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As a result, the detection of pathogenic bacteria is limited, the blindness is large, and it may mislead the investigation
At the same time, there are also many problems such as high missed detection rate, difficulty in finding new pathogenic bacteria (mold), long detection time, low detection rate, material consumption, and heavy workload, which obviously restricts the timely and accurate handling of sudden food poisoning incidents. Timeliness

Method used

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  • Dyeing indicating method used for detecting food poisoning pathogenic bacteria

Examples

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Embodiment 1

[0020] Example 1: Detection results of 45 microbial food poisoning pathogens. Of the 45 cases of microbial food poisoning, 40 cases were detected with pathogenic bacteria, and the detection rate was 88.89%. Among them, 38 cases were bacterial (2 cases were caused by mixed infection of Staphylococcus aureus and Bacillus cereus), and 2 cases were fungal. A total of 42 strains and 13 strains were isolated from 40 cases of microbial food poisoning detected, including 40 bacterial strains, 7 strains of Bacillus cereus emetic; 6 strains of Staphylococcus aureus and 6 strains of Vibrio parahaemolyticus; 4 strains each of Bacillus cereus and Escherichia coli (ETEC); 3 strains each of Vibrio alginolyticus and Escherichia coli; 2 strains each of common, Proteus mirabilis, and Salmonella typhimurium; Escherichia coli 1 plant. Two fungal strains were Aspergillus versicolor and Penicillium arcuatus, which could not be detected by conventional methods.

Embodiment 2

[0021] Example 2: Detection results of dominant bacteria and non-dominant bacteria in 45 cases of microbial food poisoning. 45 cases of microbial food poisoning samples were directly stained with smears, 37 cases of dominant bacteria and 8 cases of non-dominant bacteria were found under microscope. 37 dominant bacteria were cultivated, and the pathogenic bacteria were identified by family, genus, group and species (type), and the detection rate was 100%. 8 cases of non-dominant bacteria were selectively enriched and cultured, and the pathogenic bacteria were identified by family, genus, group, species (type), and 3 cases of pathogenic bacteria were detected, with a detection rate of 37.50%.

Embodiment 3

[0022] Example 3: Relationship between 13 kinds of pathogenic bacteria and infection of food poisoning samples. 40 cases of microbial food poisoning were detected with pathogenic bacteria, and a total of 761 samples were collected, including 122 vomitus, 180 feces, 388 suspicious food residues, 47 tableware, 2 cutting boards, 2 kitchen knives, 14 rags, 6 copies of cook manure. As a result, there were 122 vomitus. 102 pathogenic bacteria were detected, accounting for 83.61%, among which the detection rates of Staphylococcus aureus and Bacillus cereus were 100% and 93.02%, respectively. Of 180 feces, 153 pathogenic bacteria were detected, accounting for 85.00%. Among them, the detection rates of Vibrio paralyticus, Bacillus cereus, Escherichia coli EIEC and Penicillium arcuatus were all 100%; Escherichia coli ETEC , Proteus mirabilis, Bacillus cereus, Escherichia coli EPEC, and Salmonella typhimurium were 93.10%, 88.89%, 83.33%, 82.14%, and 80.00%, respectively. Thirteen kind...

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Abstract

A staining guide method used in pathogenic bacteria detection of food poisoning includes directly carrying out gramí»s staining microscopic examination on food poisoning sample to confirm dominant strain and non-dominant strain in examination sample, making preliminary report after biochemical and serological examination is done on cultured dominant colony, carrying out animal toxicity test and then making out accurate diagnosis report on dominant colony; doing the same as above on no dominant colony and making out accurate diagnosis report on no dominant colony.

Description

Technical field: [0001] The invention belongs to pathogen detection technology, in particular to a new method for rapid detection of food poisoning pathogens. Background technique: [0002] Food poisoning caused by food contamination by pathogenic bacteria (mold) is one of the most widespread and common diseases in the world today. Today, with the increasing scale and globalization of food industrial production and food trade, the sources and methods of pathogenic bacteria contaminating food are becoming more and more hidden, complex and plastic. At the same time, due to changes in population and environment, and changes in human life and behavior, the epidemiology of food poisoning is also undergoing rapid changes. Emerging again and again, the drug resistance of pathogenic bacteria is also increasing constantly. The widespread distribution of food poisoning poses a serious threat to all countries in the world. Therefore, food contamination by pathogenic bacteria and food...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/29C12Q1/02G01N33/50
Inventor 葛素君冯济富许际华裘丹红
Owner 台州市疾病预防控制中心
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