Technique for producing purple common perilla rosemary acid

A technology of rosmarinic acid and production process, applied in the field of deep processing of natural crops, can solve the problems of cumbersome methods, high cost, difficult industrialized production and the like, and achieve the effects of simple production process, low production cost and rich product specifications

Inactive Publication Date: 2006-07-05
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research about rosmarinic acid at home and abroad has shown that it has important application value in fields such as pharmacy, food, cosmetics at present, so many researchers are all studying the chemical synthesis and biosynthesis method of rosmarinic acid, but its The method is cumbersome, the cost is high, and it is difficult to realize industrial production
There are not many studies on the large-scale separation and purification of rosmarinic acid at home and abroad, so it is necessary to establish a fast and simple process for producing rosmarinic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] 20 kilograms of perilla leaves were extracted with pH 2-4 acid water at 40°C for 4 hours, and the extraction was repeated 3 times. The combined extracts were combined with XAD-4 macroporous adsorption resin (10×80cm), and then washed with 95% alcohol Desorption, obtain 4 kilograms of desorption liquids, the desorption liquid is concentrated to 0.5 kilograms under reduced pressure, promptly obtains the rosmarinic acid product that purity is 10%; The rosmarinic acid product of 200 grams of 10% purity is extracted twice with sherwood oil, The rosmarinic acid product of 50% is the rosmarinic acid product that obtains 15 gram purity of organic phase decompression evaporation; The AB-8 adsorption resin (5 * 60cm) post of the rosmarinic acid product of 4 gram 50% purity is used respectively with concentration as 30 %, 95% ethanol solution for segmental elution, 95% components were concentrated to 3mL under reduced pressure, and crystallized at 4°C to obtain 1.9 grams of rosmari...

Embodiment 2

[0011] 300 kilograms of perilla leaves were extracted with acid water of pH2-4 for 4 hours at a temperature of 40°C, and the extraction was repeated 3 times, and the combined extracts were put on an XAD-4 macroporous adsorption resin column (30×150cm), and then washed with 95% Alcohol desorption, obtains 40 kilograms of desorption liquids, decompresses and concentrates desorption liquids to 3 kilograms, obtains the rosmarinic acid product that purity is 10%; Dilutes 2 kilograms of 10% purity rosmarinic acids to 5 kilograms with distilled water, respectively Extract three times with 2 kg of petroleum ether, combine the organic phases, evaporate and concentrate the organic phases to 0.38 kg under reduced pressure to obtain a rosmarinic acid product with a purity of 50%; add 100 g of rosmarinic acid with a purity of 50% to AB-8 The adsorption resin column was eluted with ethanol solutions with a concentration of 30% and 95% respectively, and then 95% of the component eluent was co...

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Abstract

This invention relates to a production technique of perilla-derived rosmarinic acid which is accessible for large-scale industrial production. Perilla leaves serving as raw materials are dipped in acidic water and the mixture undergoes filtration with the extracted liquid adsorbed and desorbed in macroporous adsorption resin; the desorbed liquid is vacuum distilled to obtain romarinic acid with a purity of 10%; the 10%-purity rosmarinic acid product is extracted with petroleum ether and the organic phase is vacuum distilled to obtain rosmarinic acid with a purity of 50%; the 50%-purity rosmarinic acid product is gradient eluted with ethanol solutions of different concentrations in adsorption resin and the 95% eluent is vacuum-condensed and crystallized to produce a rosmarinic acid product with a purity of 95%. This invention has the advantages of simple production technique, easy operation, low cost, variable product types, security and reliability and therefore it is applicable in producing food additives, medical intermediates and cosmetics.

Description

Technical field: [0001] The invention relates to a process for large-scale industrial production of perilla rosmarinic acid, which belongs to the technical field of deep processing of natural crops. technical background: [0002] Rosmarinic acid (RosA for short) is a multifunctional phenolic acid compound, which exists in various plants of the Labiatae family, and has various biological activities. As an antioxidant, rosmarinic acid has a very strong activity of scavenging free radicals in the body, and its antioxidant activity is stronger than that of caffeic acid, chlorogenic acid, folic acid, ferulic acid, vitamin E, etc.; as an antioxidant, rosmarinic acid, Also inhibits the oxidation of low-density lipoprotein regulated by endothelial cells. Rosmarinic acid also has antiviral activity. Arda et al. found that the alcohol-water extract of Bean sprouts has anti-HIV activity, and the main substance that works is rosmarinic acid; Mazumder et al.’s research further shows tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/56
Inventor 杜桂彩杨宏徐公世李荣贵
Owner QINGDAO UNIV
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