Method for extracting high purity protein from cow milk or soybean waste water

A high-purity, whey-water technology, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve problems such as low-cost large-scale industrial production

Active Publication Date: 2006-07-12
东营瑞达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to solve the problem that the existing technology of extracting high-purity protein from milk or whey water cannot carry out large-scale industrial production at low cost

Method used

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  • Method for extracting high purity protein from cow milk or soybean waste water

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: 993 liters of semi-skimmed milk, temperature 8.2 degrees C, pH 6.6, total protein 32.5 grams per liter, BLP concentration 366 mg per liter, BLF concentration 138 milliliters per liter. Use the process route in Figure 1 and follow the steps described in the instructions to produce mixed protein powder of BLP and BLF. Stirring tank T2 contains SPEC70 resin. The adsorption time is 115 minutes. Add 90 liters of 5.5% sodium chloride brine during solution, and the solution time is 65 minutes. The solution and eluate are combined into the microfiltration unit (0.1 micron microfiltration membrane) for microfiltration to remove bacteria and fat. 136 liters of the filtrate is sent to the ultrafiltration membrane unit (30K molecular cut-off) for desalination and concentration. A concentrated solution of 0.52 liters was collected. Adopt freeze-drying method to obtain light brown green dry powder 351 grams, water content 4.3%, total protein is 93.2%, ash content 2.6%....

Embodiment 2

[0047] Example 2: 996 liters of skim milk, pH 6.7, temperature 6.6 degrees C, total protein is 32.9 grams per liter, BLP concentration is 378 mg per liter, BLF concentration is 129 mg per liter. Use the process route in Figure 1 and follow the steps described in the instructions to produce monomeric BLP protein and monomeric BLF protein. Add the SPEC70 resin stirring tank inside to absorb for 115 minutes, and the stirring speed is 60rpm. After collecting the absorbed milk, rinse the resin with RO water. Add 90 liters of first-stage brine (2.4%) to decompose for 36 minutes, stirring at a speed of 30 rpm. Collect the first stage BLP solution and add 2.3% saline to rinse the resin for 3 times. Merge the eluate into the BLP solution and use a microfiltration membrane to remove bacteria and fat.

[0048] At the same time as BLP microfiltration, 86 liters of 4.9% brine was added to the stirring tank for BLF desorption, and the stirring speed was 36 rpm. The solution time is 60 m...

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Abstract

The invention discloses a method for extracting high quality protein form milk or whey water, which adopts the stirring tank with cationic ion-exchange resin to adsorb, desorb and separately extracts the lactoferrin, oxidative enzyme protein and the mixer. It uses film separating technology to do separately condensation and highly purity. The stirring tank can adjust the stirring rotating speed at 20-100rpm; the tank inside wall has swirl-adding flag; the bottom of the tank has resin bed with filter plate; the fast guiding suction tube is positioned on the resin layer upper 8-12cm of the resin bed.

Description

technical field [0001] The invention relates to a method for extracting high-purity protein from milk or whey water Background technique [0002] The invention relates to a method for extracting high-purity lactoferrin (bovine lactoferrin, abbreviated as BLF), oxidase protein (bovine lactoperoxidase, abbreviated as BLP) and lactoferrin and oxidase protein simultaneously from milk or whey water by using a stirring tank Mixture method. Oxidase protein and lactoferrin have broad-spectrum antibacterial, antiviral and improve the immune function of the body against disease. For example, BLP can destroy the cytoplasm of bacteria and inhibit the synthesis of ribonucleic acid during the growth of bacteria. Therefore, it has an inhibitory effect on Gram-positive bacteria and Gram-negative bacteria, yeasts such as Candida albicans, and molds such as Aspergillus niger. Oxidase proteins are present in the secretions of mammals, especially milk. However, its concentration is very low...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/36
Inventor 方存林方雅悯
Owner 东营瑞达生物科技有限公司
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