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Coriolus versicolor strain and method for producing Coriolus versicolor intracellular glycopeptide using the strain

A technology of versicolor versicolor and strains, applied in the direction of fungi, fermentation, etc., which can solve the problems of low yield of glycopeptides in versicolor versicolor, low content of active ingredients, and low mycelium yield, so as to retain activity and improve yield efficiency and reduce extraction time

Inactive Publication Date: 2006-07-26
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Another object of the present invention is to provide a kind of by feed and control fermented liquid pH, to increase the Yunzhi mycelia quantity, and then improve the fermentation method of Yunzhi intracellular glycopeptide yield, solve the prior art mycelium The problem of low yield and low yield of intracellular glycopeptides;
[0011] Another object of the present invention is to provide a new method for extracting intracellular glycopeptides from Versicolor versicolor, to overcome the problems of long operation time, low yield of intracellular glycopeptides from Versicolor versicolor and low content of active ingredients in the prior art

Method used

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  • Coriolus versicolor strain and method for producing Coriolus versicolor intracellular glycopeptide using the strain

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The seed liquid is prepared from the slant culture of the strain CCTCC M204081 through two-stage liquid fermentation culture:

[0049] Incline culture:

[0050] The slant medium (g / l) is potato 200, glucose 10, sucrose 10, yeast powder 0.1, agar 2; after inoculation, culture at 27°C for 5 days;

[0051] Primary and secondary seed cultivation:

[0052] The seed medium (g / l) is glucose 30, sucrose 30, corn flour 21, bran 17, peptone 10, bean cake powder 15, dipotassium hydrogen phosphate 6, magnesium sulfate 0.6; cultivated for 50 hours after inoculation, temperature 28°C, Rotate at 170rpm to obtain seed solution.

Embodiment 2

[0054] Fermentation medium: Each liter medium contains 30g of glucose, 20g of bean cake powder, 6g of peptone, 5g of bran and inorganic salts. 30 liters of full-automatic stainless steel fermenters (produced by Wuxi Sanhai Biochemical Engineering Co., Ltd.), liquid capacity 22L, 121 ℃ of sterilization 25min, after cooling to room temperature, insert the seed liquid of embodiment 1, inoculum size is 10%, stir Speed ​​200rpm, ventilation 0.8v / v / m, temperature 27℃, tank pressure 0.5kg / cm 2 .

[0055] Fermentation was completed after 4 days of cultivation. The dry weight of the mycelium was 18.50g / l. The mycelium was extracted with broken wall hot water, filtered, concentrated under reduced pressure, and dried by alcohol precipitation to obtain 1.10g / l intracellular glycopeptide. Its total sugar was detected to be 44.57%, and the peptide was 27.92%. It was detected by HPLC, and its peak time was the same as that of the standard product. Other indicators are in line with the nati...

Embodiment 3

[0057] Fermentation medium:

[0058] Each liter of medium contains 30g of glucose, 20g of bean cake powder, 6g of peptone, 5g of bran and inorganic salts. 30-liter automatic stainless steel fermentation tank, liquid volume 20L, inoculum volume 10%, stirring speed 150rpm, ventilation 1v / v / m, temperature 26℃, tank pressure 0.5kg / cm 2 . After 30 hours of fermentation, add 1 L of glucose with a weight concentration of 20%, and at the same time use 0.1N NaOH to control the pH to 5. After 4.5 days of cultivation, the fermentation ends. The dry weight of the mycelium is 28.78g / l, and the mycelium is extracted by broken hot water , filtered, concentrated under reduced pressure, and dried by alcohol precipitation to obtain 2.0g / l intracellular glycopeptide. The total sugar was 55.15% and the peptide was 34.42%. HPLC detection showed that the peak time was the same as that of the standard product, which met the requirements of the national drug Standard WS-XG-021-2002.

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Abstract

This invention belongs to the field of drug-used microbe fermentation, specifically it relates to stictin endocellular glicotide yielding bacterium and its fermentation plating method. The originator screen and get stictin endocellular glicotide yielding bacterium: stictin (Coriolus versicolor)CCTCC M204081, which can give birth to high-strength effective composition; he also supplies a new method for optimizing fermentation process by supplementing and controlling pH value. This invention also discloses a new extraction technique about how to extract stictin endocellular glicotide from mycelium. This method can enhance the quality of mycelium and this is good to synthesize stictin endocellular glicotide, therefore the productivity is advance.

Description

technical field [0001] The invention belongs to the field of medicinal microbial fermentation, and specifically relates to a glycopeptide-producing bacterium in the versicolor versicolor lucidum cell and a fermentation and cultivation method thereof. Background technique [0002] Coriolus versicolor intracellular glycopeptides are proteoglycans extracted from the liquid cultured mycelia of the medicinal fungus Coriolus Versicolor or from the fruiting body of Versicolor versicolor. The intracellular glycopeptides of Yunzhi have a wide range of pharmacological effects, especially for The body's immune function has the function of regulating and enhancing, and it is an effective immune enhancing drug, which has become a drug for treating immunocompromised persons. Yunzhi intracellular glycopeptide can prevent and treat chronic hepatitis B, liver cirrhosis and other liver diseases, and can prevent and treat various tumor diseases such as liver cancer, breast cancer, gastric canc...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P21/00
Inventor 余晓斌胡卫珍濮文林
Owner JIANGNAN UNIV
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