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Process for producing rose with modified color

一种制造方法、蔷薇的技术，应用在蔷薇的制造领域，能够解决CHS抑制不完全、未达到完全抑制花色素苷等问题

Inactive Publication Date: 2006-09-20
SUNTORY HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In roses, although co-suppression of the chalcone synthase (CHS) gene required for anthocyanin synthesis has been reported to change flower color from red to pink (Gutterson HortScience 30:964-966 1995) , but at this time the inhibition of CHS is not complete, and the synthesis of anthocyanins has not been completely inhibited to obtain the white flower line

Method used

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  • Process for producing rose with modified color
  • Process for producing rose with modified color
  • Process for producing rose with modified color

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Example 1. Flower color measurement method

[0098] The petal color was analyzed using a spectrophotometer CM2022 [MINOLTA Co., Ltd., Japan] 10 degree vision, D65 light source measurement, and the color management software SpectraMagic [MINOLTA Co., Ltd., Japan] was used for analysis. In addition, the Royal Horticultural Society Chromatography Standard (RHSCC) number is based on the chromaticity value (CIE L*a*b* chromaticity system) obtained by visual judgment and the above-mentioned machine measurement, using the color classification system Version 2.1.1 [Japan Research Institute Co., Ltd. (Japan), Japanese Patent Application Publication No. 2002-016935] The approximate color comparison was performed. By using this system, an approximate RHSCC number can be selected objectively.

[0099] Using this method to measure the chromaticity of the petals of the so-called "blue rose" variety in the past, when calculating the approximate color in RHSCC, Blue Moon and Madame Violet ...

Embodiment 2

[0100] Example 2. Analysis of Flavonoids

[0101] 1) Extraction of petal pigment

[0102] Put 0.5g of freeze-dried petals in 50% acetonitrile (CH 3 CN), after 20 minutes of ultrasonic extraction, and then filter with a 0.45μm filter. The high performance liquid chromatography (HPLC) analysis conditions of the anthocyanin of this extract are as follows. The chromatographic column uses RSpakDE-413L (4.6mmφ×25cm, Zhaogong Co., Ltd.), the flow rate is 0.6ml / min, and the mobile phase is CH containing 0.5% trifluoroacetic acid (TFA) 3 CN / H 2 O, 10%-50% linear concentration gradient 15 minutes later, use 50% CH containing 0.5% TFA 3 CN / H 2 O performs isocratic elution for 10 minutes. For detection, a photodiode array (PHOTODIODE ARRAY) detector SPD-M10A (Shimadzu Corporation) was used to detect the wavelength range of 600-250 nm, and the ratio of each anthocyanin was calculated from the area of ​​absorbance at 520 nm.

[0103] 2) Analysis of anthocyanin

[0104] Put 0.2ml of the above fi...

Embodiment 3

[0109] Example 3. Determination of DH value

[0110] Approximately 2 g of rose petals frozen at -80°C for 1 hour or more are squeezed with a homogenizer to obtain a squeezed juice. The pH was measured with a pH meter (F-22, Horiba Manufacturing Co., Ltd.) connected to a microelectrode 6069-10C (Horiba Manufacturing Co., Ltd.).

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Abstract

A process for producing a rose, characterized in that the intrinsic metabolic pathway of rose is artificially suppressed and that a gene coding for flavonoid 3',5'-hydroxylase derived from pansy is expressed.

Description

Technical field [0001] The present invention relates to a method for manufacturing roses (including roses and roses) with new flower colors. Specifically, the present invention relates to a method for manufacturing rose, which expresses a gene encoding a flavonoid 3',5'-hydroxylase derived from pansy and encoding reduced dihydrogenase by artificially inhibiting the endogenous metabolic pathway of rose Myricetin (dihydromyricetin) dihydroflavonol (dihydroflavonol) reductase gene. Background technique [0002] The function of petals is to attract insects, birds and other pollinators that transfer pollen. Therefore, the color, shape, pattern, and fragrance of flowers are co-evolved with pollinators [Toshio Honda, etc., Modern Chemistry, May, 25- 32 (1998)]. As a result, very few flowers of one species have all the colors. For example, there is no purple to blue in rose and carnation, and there is no bright red in calamus and gentian. Flower color is the most important trait in flowe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/00A01H5/00C12N15/82
CPCC12N15/825C12N15/82C12N5/14
Inventor 田中良和福井祐子户上纯一胜元幸久水谷正子
Owner SUNTORY HLDG LTD
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